Shutian Jiang, Jing Zhang, Xueyi Jiang, Yu Tao, Jing Yang, Kanru Zhao, Nannan Li, Luping Feng, Haoran Shen, Yao Wang
{"title":"左卡尼汀预孵育可减轻小鼠卵母细胞玻璃化和解冻损伤。","authors":"Shutian Jiang, Jing Zhang, Xueyi Jiang, Yu Tao, Jing Yang, Kanru Zhao, Nannan Li, Luping Feng, Haoran Shen, Yao Wang","doi":"10.1007/s10815-025-03681-5","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Vitrification of mammalian oocytes is an important means of preserving female reproductive capacity. However, existing technical solutions cannot guarantee recovery rate, fertilization, and developmental potential of vitrified oocytes. Recognized consequences include energy consumption during vitrification and thawing processes, and damage to organelles caused by the formation of ice crystals. In this study, we explore whether incubating with levocarnitine (LC) before vitrification can improve its warming outcomes.</p><p><strong>Results: </strong>Supplementing 2 mg/mL LC can significantly improved recovery rate of warmed oocytes (p < 0.01) and shortened morphological recovery time (p < 0.001). Gene difference analysis showed that supplementing LC altered gene expression related to energy metabolism, autophagy, and cytoskeleton. Further experimental testing showed that supplementing LC increased adenosine triphosphate (ATP) levels (p < 0.05), decreased reactive oxygen species (ROS) levels (p < 0.001), and maintained the stability of the spindle chromosome structure. On this basis, both lipid oxidation (p < 0.01) and autophagy levels (p < 0.0001) in oocytes decreased, and the quality of embryos developed from warmed oocytes was improved (p < 0.05).</p><p><strong>Conclusion: </strong>Supplementation of LC to mouse oocytes before vitrification can improve their warming outcomes, which is related to the changes in lipid droplet distribution in oocytes caused by LC by improving lipid metabolism.</p>","PeriodicalId":15246,"journal":{"name":"Journal of Assisted Reproduction and Genetics","volume":" ","pages":""},"PeriodicalIF":2.7000,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Pre-incubation with levocarnitine alleviates vitrification and thawing damage in mouse oocytes.\",\"authors\":\"Shutian Jiang, Jing Zhang, Xueyi Jiang, Yu Tao, Jing Yang, Kanru Zhao, Nannan Li, Luping Feng, Haoran Shen, Yao Wang\",\"doi\":\"10.1007/s10815-025-03681-5\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>Vitrification of mammalian oocytes is an important means of preserving female reproductive capacity. However, existing technical solutions cannot guarantee recovery rate, fertilization, and developmental potential of vitrified oocytes. Recognized consequences include energy consumption during vitrification and thawing processes, and damage to organelles caused by the formation of ice crystals. In this study, we explore whether incubating with levocarnitine (LC) before vitrification can improve its warming outcomes.</p><p><strong>Results: </strong>Supplementing 2 mg/mL LC can significantly improved recovery rate of warmed oocytes (p < 0.01) and shortened morphological recovery time (p < 0.001). Gene difference analysis showed that supplementing LC altered gene expression related to energy metabolism, autophagy, and cytoskeleton. Further experimental testing showed that supplementing LC increased adenosine triphosphate (ATP) levels (p < 0.05), decreased reactive oxygen species (ROS) levels (p < 0.001), and maintained the stability of the spindle chromosome structure. On this basis, both lipid oxidation (p < 0.01) and autophagy levels (p < 0.0001) in oocytes decreased, and the quality of embryos developed from warmed oocytes was improved (p < 0.05).</p><p><strong>Conclusion: </strong>Supplementation of LC to mouse oocytes before vitrification can improve their warming outcomes, which is related to the changes in lipid droplet distribution in oocytes caused by LC by improving lipid metabolism.</p>\",\"PeriodicalId\":15246,\"journal\":{\"name\":\"Journal of Assisted Reproduction and Genetics\",\"volume\":\" \",\"pages\":\"\"},\"PeriodicalIF\":2.7000,\"publicationDate\":\"2025-09-30\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Assisted Reproduction and Genetics\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1007/s10815-025-03681-5\",\"RegionNum\":3,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"GENETICS & HEREDITY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Assisted Reproduction and Genetics","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1007/s10815-025-03681-5","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"GENETICS & HEREDITY","Score":null,"Total":0}
Pre-incubation with levocarnitine alleviates vitrification and thawing damage in mouse oocytes.
Background: Vitrification of mammalian oocytes is an important means of preserving female reproductive capacity. However, existing technical solutions cannot guarantee recovery rate, fertilization, and developmental potential of vitrified oocytes. Recognized consequences include energy consumption during vitrification and thawing processes, and damage to organelles caused by the formation of ice crystals. In this study, we explore whether incubating with levocarnitine (LC) before vitrification can improve its warming outcomes.
Results: Supplementing 2 mg/mL LC can significantly improved recovery rate of warmed oocytes (p < 0.01) and shortened morphological recovery time (p < 0.001). Gene difference analysis showed that supplementing LC altered gene expression related to energy metabolism, autophagy, and cytoskeleton. Further experimental testing showed that supplementing LC increased adenosine triphosphate (ATP) levels (p < 0.05), decreased reactive oxygen species (ROS) levels (p < 0.001), and maintained the stability of the spindle chromosome structure. On this basis, both lipid oxidation (p < 0.01) and autophagy levels (p < 0.0001) in oocytes decreased, and the quality of embryos developed from warmed oocytes was improved (p < 0.05).
Conclusion: Supplementation of LC to mouse oocytes before vitrification can improve their warming outcomes, which is related to the changes in lipid droplet distribution in oocytes caused by LC by improving lipid metabolism.
期刊介绍：
The Journal of Assisted Reproduction and Genetics publishes cellular, molecular, genetic, and epigenetic discoveries advancing our understanding of the biology and underlying mechanisms from gametogenesis to offspring health. Special emphasis is placed on the practice and evolution of assisted reproduction technologies (ARTs) with reference to the diagnosis and management of diseases affecting fertility. Our goal is to educate our readership in the translation of basic and clinical discoveries made from human or relevant animal models to the safe and efficacious practice of human ARTs. The scientific rigor and ethical standards embraced by the JARG editorial team ensures a broad international base of expertise guiding the marriage of contemporary clinical research paradigms with basic science discovery. JARG publishes original papers, minireviews, case reports, and opinion pieces often combined into special topic issues that will educate clinicians and scientists with interests in the mechanisms of human development that bear on the treatment of infertility and emerging innovations in human ARTs. The guiding principles of male and female reproductive health impacting pre- and post-conceptional viability and developmental potential are emphasized within the purview of human reproductive health in current and future generations of our species.
The journal is published in cooperation with the American Society for Reproductive Medicine, an organization of more than 8,000 physicians, researchers, nurses, technicians and other professionals dedicated to advancing knowledge and expertise in reproductive biology.
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