{"title":"2006-2021年日本健康食品处理者肠出血性大肠杆菌O157分离株抗微生物药物耐药性、毒力和质粒介导的可转移性的遗传特征","authors":"Natsuki Ohata, Mamoru Noda, Kenji Ohta, Moritaka Hatta, Tatsuya Nakayama","doi":"10.1093/jambio/lxaf245","DOIUrl":null,"url":null,"abstract":"<p><strong>Aims: </strong>The increasing prevalence of antibiotic-resistant foodborne pathogens is a serious public health concern worldwide. Enterohaemorrhagic Escherichia coli O157 (EHEC-O157) poses a high food contamination risk. This study aimed to investigate changes in the prevalence of EHEC-O157 among food handlers in Japan in 2006-2021.</p><p><strong>Methods and results: </strong>A total of 279 randomly selected isolates from 2006 to 2009, 2019, and 2021 were analysed for their antimicrobial resistance patterns and virulence genes. EHEC-O157 isolation rates in 2012-2022 were 0.0011%-0.0037%, with an average isolation rate of 0.0024% (659/27 848 713). Antimicrobial susceptibility testing showed that EHEC-O157 isolates from all years were most resistant to streptomycin (>34%) and ampicillin (>7.7%), followed by tetracycline (<5.1%). EHEC-O157 isolates resistant to chloramphenicol (>9.4%) and kanamycin (>2.6%) have been isolated since 2019. Furthermore, the detection of Shiga toxin-related genes revealed that stx2 (57.4%) was more prevalent than stx1 (6%) in all sampling years. A whole-genome analysis revealed the presence of aph(6)-ld, aph(3'')-ld, and blaTEM-1B in the multidrug-resistant isolates, suggesting that these genes contribute to streptomycin and ampicillin resistance. Furthermore, one strain was an extended-spectrum β-lactamase producer harbouring the IncFII plasmid that encodes blaCTX-M-15. Moreover, EHEC acquired a plasmid encoding blaNDM-1 from carbapenem-resistant Citrobacter freundii.</p><p><strong>Conclusions: </strong>We characterized the antimicrobial resistance and virulence factors of EHEC-O157 from food handlers. EHEC strains can easily acquire and spread antibiotic resistance. Notably, EHEC-O157 isolates from healthy individuals exhibited uninterrupted streptomycin and ampicillin resistance over the past decade. In recent years, there has been a discernible trend towards multidrug resistance of EHEC-O157.</p>","PeriodicalId":15036,"journal":{"name":"Journal of Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":3.2000,"publicationDate":"2025-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Genetic characterization of antimicrobial resistance, virulence, and plasmid-mediated transferability in Enterohaemorrhagic E. coli O157 isolates from healthy food handlers in Japan, 2006-2021.\",\"authors\":\"Natsuki Ohata, Mamoru Noda, Kenji Ohta, Moritaka Hatta, Tatsuya Nakayama\",\"doi\":\"10.1093/jambio/lxaf245\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Aims: </strong>The increasing prevalence of antibiotic-resistant foodborne pathogens is a serious public health concern worldwide. Enterohaemorrhagic Escherichia coli O157 (EHEC-O157) poses a high food contamination risk. This study aimed to investigate changes in the prevalence of EHEC-O157 among food handlers in Japan in 2006-2021.</p><p><strong>Methods and results: </strong>A total of 279 randomly selected isolates from 2006 to 2009, 2019, and 2021 were analysed for their antimicrobial resistance patterns and virulence genes. EHEC-O157 isolation rates in 2012-2022 were 0.0011%-0.0037%, with an average isolation rate of 0.0024% (659/27 848 713). Antimicrobial susceptibility testing showed that EHEC-O157 isolates from all years were most resistant to streptomycin (>34%) and ampicillin (>7.7%), followed by tetracycline (<5.1%). EHEC-O157 isolates resistant to chloramphenicol (>9.4%) and kanamycin (>2.6%) have been isolated since 2019. Furthermore, the detection of Shiga toxin-related genes revealed that stx2 (57.4%) was more prevalent than stx1 (6%) in all sampling years. A whole-genome analysis revealed the presence of aph(6)-ld, aph(3'')-ld, and blaTEM-1B in the multidrug-resistant isolates, suggesting that these genes contribute to streptomycin and ampicillin resistance. Furthermore, one strain was an extended-spectrum β-lactamase producer harbouring the IncFII plasmid that encodes blaCTX-M-15. Moreover, EHEC acquired a plasmid encoding blaNDM-1 from carbapenem-resistant Citrobacter freundii.</p><p><strong>Conclusions: </strong>We characterized the antimicrobial resistance and virulence factors of EHEC-O157 from food handlers. EHEC strains can easily acquire and spread antibiotic resistance. Notably, EHEC-O157 isolates from healthy individuals exhibited uninterrupted streptomycin and ampicillin resistance over the past decade. In recent years, there has been a discernible trend towards multidrug resistance of EHEC-O157.</p>\",\"PeriodicalId\":15036,\"journal\":{\"name\":\"Journal of Applied Microbiology\",\"volume\":\" \",\"pages\":\"\"},\"PeriodicalIF\":3.2000,\"publicationDate\":\"2025-10-06\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Applied Microbiology\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1093/jambio/lxaf245\",\"RegionNum\":3,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"BIOTECHNOLOGY & APPLIED MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Applied Microbiology","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1093/jambio/lxaf245","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
Genetic characterization of antimicrobial resistance, virulence, and plasmid-mediated transferability in Enterohaemorrhagic E. coli O157 isolates from healthy food handlers in Japan, 2006-2021.
Aims: The increasing prevalence of antibiotic-resistant foodborne pathogens is a serious public health concern worldwide. Enterohaemorrhagic Escherichia coli O157 (EHEC-O157) poses a high food contamination risk. This study aimed to investigate changes in the prevalence of EHEC-O157 among food handlers in Japan in 2006-2021.
Methods and results: A total of 279 randomly selected isolates from 2006 to 2009, 2019, and 2021 were analysed for their antimicrobial resistance patterns and virulence genes. EHEC-O157 isolation rates in 2012-2022 were 0.0011%-0.0037%, with an average isolation rate of 0.0024% (659/27 848 713). Antimicrobial susceptibility testing showed that EHEC-O157 isolates from all years were most resistant to streptomycin (>34%) and ampicillin (>7.7%), followed by tetracycline (<5.1%). EHEC-O157 isolates resistant to chloramphenicol (>9.4%) and kanamycin (>2.6%) have been isolated since 2019. Furthermore, the detection of Shiga toxin-related genes revealed that stx2 (57.4%) was more prevalent than stx1 (6%) in all sampling years. A whole-genome analysis revealed the presence of aph(6)-ld, aph(3'')-ld, and blaTEM-1B in the multidrug-resistant isolates, suggesting that these genes contribute to streptomycin and ampicillin resistance. Furthermore, one strain was an extended-spectrum β-lactamase producer harbouring the IncFII plasmid that encodes blaCTX-M-15. Moreover, EHEC acquired a plasmid encoding blaNDM-1 from carbapenem-resistant Citrobacter freundii.
Conclusions: We characterized the antimicrobial resistance and virulence factors of EHEC-O157 from food handlers. EHEC strains can easily acquire and spread antibiotic resistance. Notably, EHEC-O157 isolates from healthy individuals exhibited uninterrupted streptomycin and ampicillin resistance over the past decade. In recent years, there has been a discernible trend towards multidrug resistance of EHEC-O157.
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Journal of & Letters in Applied Microbiology are two of the flagship research journals of the Society for Applied Microbiology (SfAM). For more than 75 years they have been publishing top quality research and reviews in the broad field of applied microbiology. The journals are provided to all SfAM members as well as having a global online readership totalling more than 500,000 downloads per year in more than 200 countries. Submitting authors can expect fast decision and publication times, averaging 33 days to first decision and 34 days from acceptance to online publication. There are no page charges.
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