从黄海绿潮中占主导地位的浒苔藻中分离出的藻异单胞菌和藻异单胞菌。

IF 2 3区 生物学 Q4 MICROBIOLOGY
Xiaojing Lv, Jin Zhao, Peng Jiang
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引用次数: 0

摘要

菌株A079T和A081T分别从黄海最大绿潮的优势种增殖Ulva海藻中分离得到。两株菌株均为革兰氏阴性,需氧且通过单极鞭毛运动。两菌株的主要脂肪酸为C16:0、C17:1 ω8c、C18:1 ω7c和特征3 (C16:1 ω7c和/或C16:1 ω6c)。菌株A079T细胞呈椭圆形或短杆状。菌株A081T细胞呈棒状。A079T在10-37℃、pH 6.0-10.0和1-12% (w/v) NaCl条件下生长。A081T在10-40℃、pH 6.0-11.0和1-12% (w/v) NaCl条件下生长。基于16S rRNA基因和全基因组序列的系统发育分析表明,菌株A079T和A081T属于Alteromonas属。菌株A079T、A081T与相关异单胞菌的核苷酸同源性平均值为68.98 ~ 88.75%,数字DNA-DNA杂交值为20.40 ~ 36.40%。比较基因组分析发现A079T具有独特的RiPP-like (TP-1161)簇。菌株A089T显示了利用ulvan的潜力,并在该菌株中预测了一个酰基-高丝氨酸内酯合成基因簇。这些发现揭示了藻圈适应的可能机制。表型、化学分类、系统发育和基因组学证据表明,菌株A079T和A081T代表了交替单胞菌属的两个新种,分别命名为Alteromonas algae sp. nov(型菌株A079T=MCCC 1K09912T=KCTC 18157T)和Alteromonas ulvae sp. nov(型菌株A081T=MCCC 1K09913T=KCTC 18158T)。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Alteromonas algae sp. nov. and Alteromonas ulvae sp. nov., isolated from the seaweed Ulva prolifera dominating green tides in the Yellow Sea.

Strains A079T and A081T were isolated from the floating seaweed Ulva prolifera, which is the dominant species of the world's largest green tides in the Yellow Sea. Both strains were Gram-stain-negative, aerobic and motile via a single polar flagellum. The major fatty acids of the two strains were C16:0, C17:1 ω8c, C18:1 ω7c and summed feature 3 (C16:1 ω7c and/or C16:1 ω6c). Cells of the strain A079T were oval or short rod-shaped. Cells of the strain A081T were rod-shaped. A079T grew at 10-37 °C, pH 6.0-10.0 and 1-12% (w/v) NaCl. A081T grew at 10-40 °C, pH 6.0-11.0 and 1-12% (w/v) NaCl. Phylogenetic analyses based on 16S rRNA genes and whole-genome sequences revealed that strains A079T and A081T belong to the genus Alteromonas. The average nucleotide identity and digital DNA-DNA hybridization values between strains A079T, A081T and related Alteromonas species ranged from 68.98 to 88.75% and 20.40 to 36.40%, respectively. Comparative genomic analysis discovered that A079T possessed a unique RiPP-like (TP-1161) cluster. Strain A089T exhibited the potential to utilize ulvan, and an acyl-homoserine lactone synthesis gene cluster was predicted in the strain. These findings reveal possible mechanisms for phycosphere adaptation. Phenotypic, chemotaxonomic, phylogenetic and genomic evidence support that strains A079T and A081T represent two novel species of the genus Alteromonas, for which the names Alteromonas algae sp. nov. (type strain A079T=MCCC 1K09912T=KCTC 18157T) and Alteromonas ulvae sp. nov. (type strain A081T=MCCC 1K09913T=KCTC 18158T) are proposed.

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来源期刊
CiteScore
5.20
自引率
21.40%
发文量
426
审稿时长
1 months
期刊介绍: Published by the Microbiology Society and owned by the International Committee on Systematics of Prokaryotes (ICSP), a committee of the Bacteriology and Applied Microbiology Division of the International Union of Microbiological Societies, International Journal of Systematic and Evolutionary Microbiology is the leading forum for the publication of novel microbial taxa and the ICSP’s official journal of record for prokaryotic names. The journal welcomes high-quality research on all aspects of microbial evolution, phylogenetics and systematics, encouraging submissions on all prokaryotes, yeasts, microfungi, protozoa and microalgae across the full breadth of systematics including: Identification, characterisation and culture preservation Microbial evolution and biodiversity Molecular environmental work with strong taxonomic or evolutionary content Nomenclature Taxonomy and phylogenetics.
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