牛磺酸去氧胆酸(TCDCA)通过LPAR1调控Buga综合征的保护机制

IF 3.4 3区 医学 Q2 MEDICINE, RESEARCH & EXPERIMENTAL
Zeyu Guan, XiaoGao Wang, Ran Lu, Chao Xu, Erchang Zhu, Shiyuan Chen
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引用次数: 0

摘要

Budd-Chiari综合征(BCS)是一种罕见但临床意义重大的疾病,其病因尚不清楚。因此,本研究探讨牛磺酸去氧胆酸(TCDCA)对BCS中人脐静脉内皮细胞(HUVECs)的影响。经TCDCA治疗后,检测HUVECs的增殖、凋亡、迁移、炎症反应和血管生成,同时检测cAMP水平。进一步进行转录组测序。在HUVECs中沉默LPAR1以探索其功能作用。结果显示,与对照组相比,huvec中的LPAR1水平显著升高。沉默LPAR1抑制细胞增殖、迁移、炎症和血管生成,同时促进细胞凋亡和增加cAMP水平。来自BCS患者的临床样本显示LPAR1显著上调,支持我们的体外研究结果。我们的研究结果表明,TCDCA通过调节LPAR1在BCS中起关键作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
The protective mechanism of taurine deoxycholic acid (TCDCA) through LPAR1 regulation in Buga syndrome.

The etiology of Budd-Chiari syndrome (BCS), an uncommon but clinically significant condition, remains unclear. Therefore, this study investigates how taurodeoxycholic acid (TCDCA) influences human umbilical vein endothelial cells (HUVECs) in BCS. Following TCDCA treatment, the proliferation, apoptosis, migration, inflammatory response, and angiogenesis of HUVECs were examined, along with the levels of cAMP. Transcriptomic sequencing was further performed. Silencing LPAR1 in HUVECs was performed to explore its functional role. The results revealed significantly higher LPAR1 levels in HUVECs compared to controls. Silencing LPAR1 inhibited cell proliferation, migration, inflammation, and angiogenesis, while promoting apoptosis and increasing cAMP levels. Clinical samples from BCS patients showed significant upregulation of LPAR1, supporting our in vitro findings. Our findings suggest that TCDCA plays a pivotal role in BCS by regulating LPAR1.

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来源期刊
European Journal of Medical Research
European Journal of Medical Research 医学-医学:研究与实验
CiteScore
3.20
自引率
0.00%
发文量
247
审稿时长
>12 weeks
期刊介绍: European Journal of Medical Research publishes translational and clinical research of international interest across all medical disciplines, enabling clinicians and other researchers to learn about developments and innovations within these disciplines and across the boundaries between disciplines. The journal publishes high quality research and reviews and aims to ensure that the results of all well-conducted research are published, regardless of their outcome.
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