CCDC137/DGCR8轴通过激活AKT/mTOR信号通路促进肝细胞癌的有氧糖酵解。

IF 3.4 3区 医学 Q2 MEDICINE, RESEARCH & EXPERIMENTAL
Zhiying Xu, Wei Shi, Jialun Ren, Qifei Zou, Mingming Fan, Yiran Li, Dong Jiang
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引用次数: 0

摘要

肝细胞癌(HCC)是全球癌症死亡的主要原因。了解其分子机制对于开发有效的治疗方法至关重要。方法:使用r中的“limma”工具分析肝癌基因组图谱(TCGA)-肝细胞癌(LIHC)和GSE101685数据集中的差异表达基因(DEGs)。加权基因共表达网络分析(WGCNA)确定了关键的绿宝石模块。生物信息学分析CCDC137的预后意义及表达。功能分析评估了CCDC137对细胞行为和肿瘤生长的影响。我们还研究了CCDC137和DGCR8之间的联系及其对AKT/mTOR信号通路和糖酵解的影响。结果:共鉴定出670个重叠的deg,发现位于绿松石模块内的CCDC137与HCC显著相关。CCDC137在HCC中表达上调,与较差的预后结果相关。实验验证表明,CCDC137敲低可显著降低HCC细胞的增殖、迁移、侵袭和肿瘤生长。在机制上,CCDC137可能通过调节AKT/mTOR信号通路促进有氧糖酵解,可能通过其与DGCR8的相互作用介导。结论:这些研究结果表明,CCDC137/DGCR8轴可能通过AKT/mTOR途径调节细胞代谢,从而促进HCC的进展。靶向这一调控网络可能为未来HCC治疗探索提供有希望的方向。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
CCDC137/DGCR8 axis promotes aerobic glycolysis in hepatocellular carcinoma via activation of the AKT/mTOR signaling pathway.

Introduction: Hepatocellular carcinoma (HCC) is a leading cause of global cancer fatality. Understanding its molecular mechanisms is crucial for developing effective treatments.

Methods: Differentially expressed genes (DEGs) in the cancer genome atlas (TCGA)-liver hepatocellular carcinoma (LIHC) and GSE101685 data sets were analyzed using the "limma" tool in R. Weighted Gene Co-expression Network Analysis (WGCNA) identified the key turquoise module. Bioinformatics analyzed the prognostic significance and expression of CCDC137. Functional analyses assessed the effects of CCDC137 on cell behavior and tumor growth. The connection between CCDC137 and DGCR8 and their impact on the AKT/mTOR signaling pathway and glycolysis were also examined.

Results: A total of 670 overlapping DEGs were identified, and CCDC137, located within the turquoise module, was found to be significantly associated with HCC. CCDC137 was upregulated in HCC, correlating with worse prognostic outcomes. Experimental validation demonstrated that CCDC137 knockdown significantly reduced HCC cell proliferation, migration, invasion, and tumor growth. Mechanistically, CCDC137 may promote aerobic glycolysis through modulation of the AKT/mTOR signaling pathway, potentially mediated via its interaction with DGCR8.

Conclusions: These findings suggest that the CCDC137/DGCR8 axis may contribute to HCC progression by regulating cellular metabolism through the AKT/mTOR pathway. Targeting this regulatory network may offer a promising direction for future therapeutic exploration in HCC.

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来源期刊
European Journal of Medical Research
European Journal of Medical Research 医学-医学:研究与实验
CiteScore
3.20
自引率
0.00%
发文量
247
审稿时长
>12 weeks
期刊介绍: European Journal of Medical Research publishes translational and clinical research of international interest across all medical disciplines, enabling clinicians and other researchers to learn about developments and innovations within these disciplines and across the boundaries between disciplines. The journal publishes high quality research and reviews and aims to ensure that the results of all well-conducted research are published, regardless of their outcome.
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