Muhamad Ali K Shakhatreh, Hana Hamad Rabaiah, Omar F Khabour, Jacob H Jacob, Karem H Alzoubi
{"title":"粪肠球菌临床菌株毒力因子及药敏检测。","authors":"Muhamad Ali K Shakhatreh, Hana Hamad Rabaiah, Omar F Khabour, Jacob H Jacob, Karem H Alzoubi","doi":"10.2174/0113892010388498250818114856","DOIUrl":null,"url":null,"abstract":"<p><strong>Introduction: </strong>Enterococci are common commensals in the gastrointestinal tract and are opportunistic organisms that can cause urinary tract infections, bacteremia, endocarditis, and pelvic infections. The study aimed to investigate antibiotic resistance, biofilm formation, and the presence and expression of virulence factors in clinical isolates of E. faecalis from Jordan.</p><p><strong>Methods: </strong>Clinical isolates (n=89) of E. faecalis isolated from patients in Jordan were collected. Antibiotic resistance, biofilm formation, and the presence/expression of virulence genes asa1, gelE, esp, cylA, and efaA were examined.</p><p><strong>Results: </strong>High resistance of E. faecalis was detected for ampicillin (98.9%), followed by quinupristin-dalfopristin (96.6%), tetracycline (83.1%), erythromycin (78.6%), and rifampin (68.5%). Most isolates (93.3%) were found to be biofilm producers. The prevalence of virulence genes was efaA (77.5%), asa1 (77.5%), gelE (69.7%), esp (50.6%), and cylA (30%). About 25.8% of the isolates were found to be gelatinase producers. In addition, hemolysin production was observed in 37.1% of the isolates.</p><p><strong>Discussion: </strong>The esp gene was associated with tetracycline resistance. The asa1 gene was associated with susceptibility to vancomycin. CylA was associated with resistance to tetracycline and erythromycin, as well as susceptibility to ciprofloxacin and gentamicin. The presence of gelE was associated with susceptibility to chloramphenicol, tetracycline, levofloxacin, and erythromycin. The cylA gene was associated with esp and asa1 genes, while the efaA was found to be associated with gelE and asa1 genes (P<0.05). Finally, biofilm formation was not associated with antimicrobial resistance (P > 0.05).</p><p><strong>Conclusions: </strong>The antibiotic resistance profiles and associated genes of E. faecalis isolates from Jordanian patients were reported. The efaA, asa1, and gelE virulence genes were highly prevalent among the isolates. The present findings can be used in the management of E. faecalis infection in Jordan.</p>","PeriodicalId":10881,"journal":{"name":"Current pharmaceutical biotechnology","volume":" ","pages":""},"PeriodicalIF":2.6000,"publicationDate":"2025-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Detection of Virulence Factors and Antimicrobial Susceptibility among Clinical Strains of Enterococcus faecalis.\",\"authors\":\"Muhamad Ali K Shakhatreh, Hana Hamad Rabaiah, Omar F Khabour, Jacob H Jacob, Karem H Alzoubi\",\"doi\":\"10.2174/0113892010388498250818114856\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Introduction: </strong>Enterococci are common commensals in the gastrointestinal tract and are opportunistic organisms that can cause urinary tract infections, bacteremia, endocarditis, and pelvic infections. The study aimed to investigate antibiotic resistance, biofilm formation, and the presence and expression of virulence factors in clinical isolates of E. faecalis from Jordan.</p><p><strong>Methods: </strong>Clinical isolates (n=89) of E. faecalis isolated from patients in Jordan were collected. Antibiotic resistance, biofilm formation, and the presence/expression of virulence genes asa1, gelE, esp, cylA, and efaA were examined.</p><p><strong>Results: </strong>High resistance of E. faecalis was detected for ampicillin (98.9%), followed by quinupristin-dalfopristin (96.6%), tetracycline (83.1%), erythromycin (78.6%), and rifampin (68.5%). Most isolates (93.3%) were found to be biofilm producers. The prevalence of virulence genes was efaA (77.5%), asa1 (77.5%), gelE (69.7%), esp (50.6%), and cylA (30%). About 25.8% of the isolates were found to be gelatinase producers. In addition, hemolysin production was observed in 37.1% of the isolates.</p><p><strong>Discussion: </strong>The esp gene was associated with tetracycline resistance. The asa1 gene was associated with susceptibility to vancomycin. CylA was associated with resistance to tetracycline and erythromycin, as well as susceptibility to ciprofloxacin and gentamicin. The presence of gelE was associated with susceptibility to chloramphenicol, tetracycline, levofloxacin, and erythromycin. The cylA gene was associated with esp and asa1 genes, while the efaA was found to be associated with gelE and asa1 genes (P<0.05). Finally, biofilm formation was not associated with antimicrobial resistance (P > 0.05).</p><p><strong>Conclusions: </strong>The antibiotic resistance profiles and associated genes of E. faecalis isolates from Jordanian patients were reported. The efaA, asa1, and gelE virulence genes were highly prevalent among the isolates. 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Detection of Virulence Factors and Antimicrobial Susceptibility among Clinical Strains of Enterococcus faecalis.
Introduction: Enterococci are common commensals in the gastrointestinal tract and are opportunistic organisms that can cause urinary tract infections, bacteremia, endocarditis, and pelvic infections. The study aimed to investigate antibiotic resistance, biofilm formation, and the presence and expression of virulence factors in clinical isolates of E. faecalis from Jordan.
Methods: Clinical isolates (n=89) of E. faecalis isolated from patients in Jordan were collected. Antibiotic resistance, biofilm formation, and the presence/expression of virulence genes asa1, gelE, esp, cylA, and efaA were examined.
Results: High resistance of E. faecalis was detected for ampicillin (98.9%), followed by quinupristin-dalfopristin (96.6%), tetracycline (83.1%), erythromycin (78.6%), and rifampin (68.5%). Most isolates (93.3%) were found to be biofilm producers. The prevalence of virulence genes was efaA (77.5%), asa1 (77.5%), gelE (69.7%), esp (50.6%), and cylA (30%). About 25.8% of the isolates were found to be gelatinase producers. In addition, hemolysin production was observed in 37.1% of the isolates.
Discussion: The esp gene was associated with tetracycline resistance. The asa1 gene was associated with susceptibility to vancomycin. CylA was associated with resistance to tetracycline and erythromycin, as well as susceptibility to ciprofloxacin and gentamicin. The presence of gelE was associated with susceptibility to chloramphenicol, tetracycline, levofloxacin, and erythromycin. The cylA gene was associated with esp and asa1 genes, while the efaA was found to be associated with gelE and asa1 genes (P<0.05). Finally, biofilm formation was not associated with antimicrobial resistance (P > 0.05).
Conclusions: The antibiotic resistance profiles and associated genes of E. faecalis isolates from Jordanian patients were reported. The efaA, asa1, and gelE virulence genes were highly prevalent among the isolates. The present findings can be used in the management of E. faecalis infection in Jordan.
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Current Pharmaceutical Biotechnology aims to cover all the latest and outstanding developments in Pharmaceutical Biotechnology. Each issue of the journal includes timely in-depth reviews, original research articles and letters written by leaders in the field, covering a range of current topics in scientific areas of Pharmaceutical Biotechnology. Invited and unsolicited review articles are welcome. The journal encourages contributions describing research at the interface of drug discovery and pharmacological applications, involving in vitro investigations and pre-clinical or clinical studies. Scientific areas within the scope of the journal include pharmaceutical chemistry, biochemistry and genetics, molecular and cellular biology, and polymer and materials sciences as they relate to pharmaceutical science and biotechnology. In addition, the journal also considers comprehensive studies and research advances pertaining food chemistry with pharmaceutical implication. Areas of interest include:
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