巴西散装牛乳样品中细菌多样性的评估。

IF 1.9 4区 生物学 Q3 MICROBIOLOGY
Rafaela Martins Morasi, Filippo Biscarini, Chiara Gini, Fabrizio Ceciliani, Cristina Lecchi, Anelise Salina, André Thaler Neto, Hélio Langoni, Clarice Gebara, Bruna Lourenço Crippa, Nathália Cristina Cirone Silva
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引用次数: 0

摘要

本研究旨在利用16s rRNA基因测序分析和比较来自巴西5个地区的散装储罐牛奶(BTM)样品中的细菌多样性,并将这种多样性与体细胞计数(SCC)值相关联。通过下一代测序(NGS)对来自圣卡塔琳娜州、圣保罗州、米纳斯吉拉斯州、Goiás、马拉尼赫州、帕尔州和托坎廷斯州的57份样品进行测序。结果显示,细菌共有1界4门6纲14目28科43属,其中莫拉菌科(22.3%)、链球菌科(14.1%)、醋酸杆菌科(13.8%)、假单胞菌科(11.0%)和肠球菌科(9.0%)为主科,不动杆菌属(22.3%)、假单胞菌属(11.7%)和醋酸杆菌属(8.1%)为主科。与Goiás(作为比较区域间α多样性的基线)相比,maranh和para 的α多样性差异显著,Fisher、Chao1和ACE指数显示(p 0.05), β多样性确实表现出显著差异(p 0.05)
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Assessment of bacterial diversity in bovine bulk tank milk samples from Brazil.

This research aimed to analyze and compare bacterial diversity in bulk tank milk (BTM) samples from five Brazilian regions using 16 S rRNA gene sequencing, while correlating this diversity with Somatic Cell Count (SCC) values. A total of 57 samples from Santa Catarina, São Paulo, Minas Gerais, Goiás, Maranhão, Pará, and Tocantins were sequenced through Next Generation Sequencing (NGS). The analysis revealed one kingdom, four phyla, six classes, 14 orders, 28 families, and 43 genera, with Moraxellaceae (22.3%), Streptococcaceae (14.1%), Acetobacteraceae (13.8%), Pseudomonadaceae (11.0%), and Enterococcaceae (9.0%) as the main families, and Acinetobacter (22.3%), Pseudomonas (11.7%), and Acetobacter (8.1%) as the main genera. Alpha diversity differed significantly in Maranhão and Pará compared to Goiás (set as baseline for comparing alpha diversity across regions), as indicated by Fisher, Chao1, and ACE indices (p < 0.05). In terms of beta diversity, significant differences (p < 0.001) were found among the states, highlighting the heterogeneity of these communities inside the country. While high and low SCC groups showed no significant differences in alpha diversity (p > 0.05), beta diversity did exhibit significant variation (p < 0.001). Tracking the bacterial diversity and SCC found in BTM samples using advanced molecular technologies allowed us to understand microbial diversity for future strategy development, consequently improving the quality and safety of the milk produced on Brazilian farms.

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来源期刊
Brazilian Journal of Microbiology
Brazilian Journal of Microbiology 生物-微生物学
CiteScore
4.10
自引率
4.50%
发文量
216
审稿时长
1.0 months
期刊介绍: The Brazilian Journal of Microbiology is an international peer reviewed journal that covers a wide-range of research on fundamental and applied aspects of microbiology. The journal considers for publication original research articles, short communications, reviews, and letters to the editor, that may be submitted to the following sections: Biotechnology and Industrial Microbiology, Food Microbiology, Bacterial and Fungal Pathogenesis, Clinical Microbiology, Environmental Microbiology, Veterinary Microbiology, Fungal and Bacterial Physiology, Bacterial, Fungal and Virus Molecular Biology, Education in Microbiology. For more details on each section, please check out the instructions for authors. The journal is the official publication of the Brazilian Society of Microbiology and currently publishes 4 issues per year.
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