组织工程中HB-EGF固定的肝素功能化支架。

IF 9.6 2区 医学 Q1 ENGINEERING, BIOMEDICAL
Bowu Peng, Huajian Chen, Chengyu Lu, Tianjiao Zeng, Man Wang, Toru Yoshitomi, Naoki Kawazoe, Yingnan Yang, Guoping Chen
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引用次数: 0

摘要

在支架中引入生长因子(GFs)来模拟体内微环境是一种很有前途的组织工程方法。在本研究中,设计了一种肝素功能化支架来模拟细胞外基质(ECM)的GFs储库功能。肝素结合的表皮生长因子样生长因子(HB-EGF)由于其具有肝素结合结构域,可被肝素功能化支架有效地捕获。此外,肝素与肝素结合结构域之间的强相互作用使固定化HB-EGF在支架中具有良好的长时间稳定性。用HB-EGF固定的肝素功能化支架可促进细胞粘附并促进人间充质干细胞(hMSCs)的增殖,但在增殖过程中不会诱导其分化。这些结果表明,固定化HB-EGF在不触发自发分化的情况下对hMSCs的增殖有促进作用,该系统是一种很有前景的促进支架中干细胞增殖的策略。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
A Heparin-Functionalized Scaffold with HB-EGF Immobilization for Tissue Engineering.

The introduction of growth factors (GFs) into scaffolds to mimic the in vivo microenvironment is a promising approach for tissue engineering. In this study, a heparin-functionalized scaffold is designed to mimic the GFs reservoir function of extracellular matrix (ECM). Owing to its heparin-binding domain, heparin-binding epidermal growth factor-like growth factor (HB-EGF) is effectively and spatially captured by heparin-functionalized scaffold. Furthermore, the strong interaction between heparin and heparin-binding domain confers excellent stability of the immobilized HB-EGF in scaffold over a long period. The heparin-functionalized scaffold immobilized with HB-EGF facilitates cell adhesion and promotes proliferation of human mesenchymal stem cells (hMSCs), while not inducing their differentiation during proliferation. These results indicate that the immobilized HB-EGF has a promotive effect on proliferation of hMSCs without triggering spontaneous differentiation, and the system shows as a promising strategy to enhance stem cells proliferation in scaffolds.

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来源期刊
Advanced Healthcare Materials
Advanced Healthcare Materials 工程技术-生物材料
CiteScore
14.40
自引率
3.00%
发文量
600
审稿时长
1.8 months
期刊介绍: Advanced Healthcare Materials, a distinguished member of the esteemed Advanced portfolio, has been dedicated to disseminating cutting-edge research on materials, devices, and technologies for enhancing human well-being for over ten years. As a comprehensive journal, it encompasses a wide range of disciplines such as biomaterials, biointerfaces, nanomedicine and nanotechnology, tissue engineering, and regenerative medicine.
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