{"title":"组织工程中HB-EGF固定的肝素功能化支架。","authors":"Bowu Peng, Huajian Chen, Chengyu Lu, Tianjiao Zeng, Man Wang, Toru Yoshitomi, Naoki Kawazoe, Yingnan Yang, Guoping Chen","doi":"10.1002/adhm.202502771","DOIUrl":null,"url":null,"abstract":"<p><p>The introduction of growth factors (GFs) into scaffolds to mimic the in vivo microenvironment is a promising approach for tissue engineering. In this study, a heparin-functionalized scaffold is designed to mimic the GFs reservoir function of extracellular matrix (ECM). Owing to its heparin-binding domain, heparin-binding epidermal growth factor-like growth factor (HB-EGF) is effectively and spatially captured by heparin-functionalized scaffold. Furthermore, the strong interaction between heparin and heparin-binding domain confers excellent stability of the immobilized HB-EGF in scaffold over a long period. The heparin-functionalized scaffold immobilized with HB-EGF facilitates cell adhesion and promotes proliferation of human mesenchymal stem cells (hMSCs), while not inducing their differentiation during proliferation. These results indicate that the immobilized HB-EGF has a promotive effect on proliferation of hMSCs without triggering spontaneous differentiation, and the system shows as a promising strategy to enhance stem cells proliferation in scaffolds.</p>","PeriodicalId":113,"journal":{"name":"Advanced Healthcare Materials","volume":" ","pages":"e02771"},"PeriodicalIF":9.6000,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"A Heparin-Functionalized Scaffold with HB-EGF Immobilization for Tissue Engineering.\",\"authors\":\"Bowu Peng, Huajian Chen, Chengyu Lu, Tianjiao Zeng, Man Wang, Toru Yoshitomi, Naoki Kawazoe, Yingnan Yang, Guoping Chen\",\"doi\":\"10.1002/adhm.202502771\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>The introduction of growth factors (GFs) into scaffolds to mimic the in vivo microenvironment is a promising approach for tissue engineering. In this study, a heparin-functionalized scaffold is designed to mimic the GFs reservoir function of extracellular matrix (ECM). Owing to its heparin-binding domain, heparin-binding epidermal growth factor-like growth factor (HB-EGF) is effectively and spatially captured by heparin-functionalized scaffold. Furthermore, the strong interaction between heparin and heparin-binding domain confers excellent stability of the immobilized HB-EGF in scaffold over a long period. The heparin-functionalized scaffold immobilized with HB-EGF facilitates cell adhesion and promotes proliferation of human mesenchymal stem cells (hMSCs), while not inducing their differentiation during proliferation. These results indicate that the immobilized HB-EGF has a promotive effect on proliferation of hMSCs without triggering spontaneous differentiation, and the system shows as a promising strategy to enhance stem cells proliferation in scaffolds.</p>\",\"PeriodicalId\":113,\"journal\":{\"name\":\"Advanced Healthcare Materials\",\"volume\":\" \",\"pages\":\"e02771\"},\"PeriodicalIF\":9.6000,\"publicationDate\":\"2025-10-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Advanced Healthcare Materials\",\"FirstCategoryId\":\"5\",\"ListUrlMain\":\"https://doi.org/10.1002/adhm.202502771\",\"RegionNum\":2,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"ENGINEERING, BIOMEDICAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Advanced Healthcare Materials","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.1002/adhm.202502771","RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"ENGINEERING, BIOMEDICAL","Score":null,"Total":0}
A Heparin-Functionalized Scaffold with HB-EGF Immobilization for Tissue Engineering.
The introduction of growth factors (GFs) into scaffolds to mimic the in vivo microenvironment is a promising approach for tissue engineering. In this study, a heparin-functionalized scaffold is designed to mimic the GFs reservoir function of extracellular matrix (ECM). Owing to its heparin-binding domain, heparin-binding epidermal growth factor-like growth factor (HB-EGF) is effectively and spatially captured by heparin-functionalized scaffold. Furthermore, the strong interaction between heparin and heparin-binding domain confers excellent stability of the immobilized HB-EGF in scaffold over a long period. The heparin-functionalized scaffold immobilized with HB-EGF facilitates cell adhesion and promotes proliferation of human mesenchymal stem cells (hMSCs), while not inducing their differentiation during proliferation. These results indicate that the immobilized HB-EGF has a promotive effect on proliferation of hMSCs without triggering spontaneous differentiation, and the system shows as a promising strategy to enhance stem cells proliferation in scaffolds.
期刊介绍:
Advanced Healthcare Materials, a distinguished member of the esteemed Advanced portfolio, has been dedicated to disseminating cutting-edge research on materials, devices, and technologies for enhancing human well-being for over ten years. As a comprehensive journal, it encompasses a wide range of disciplines such as biomaterials, biointerfaces, nanomedicine and nanotechnology, tissue engineering, and regenerative medicine.