黄褐斑病理特征的无创评估:双光子显微镜与反射共聚焦显微镜的对比

IF 2.6 3区 医学 Q2 CELL BIOLOGY
Xiaoli Ning, Jungang Yang, Hongfei Ouyang, Lingfan Jiang, Jiahui Han, Ziyuan Tian, Jingkai Xu, Yujun Sheng, Xianbo Zuo, Yong Cui
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引用次数: 0

摘要

黄褐斑是一种难治性色素沉着症,需要非侵入性工具来进行准确的病理评估。本研究比较了双光子显微镜(TPM)和反射共聚焦显微镜(RCM)在黄褐斑病理的体内表征。在本横断面研究中,对130例黄褐斑患者的TPM和RCM特征及成像清晰度进行了评估。将tpm量化的表皮黑色素、黑色素指数(melanin index, MI)和个体类型角(individual typogy angle, ITA)进行Spearman相关分析。还比较了活动性和稳定性疾病阶段的特征。TPM和RCM在检测表皮黑色素增加(κ = 0.651)、真皮-表皮交界处(DEJ)黑色素细胞活化(κ = 0.711)和网脊变平(κ = 0.691)(均p <; 0.001)方面表现出基本一致。TPM在显示细胞内黑色素分布、DEJ下的下垂黑色素细胞和太阳弹性变性方面表现出色,而RCM在DEJ处更好地识别活化的黑色素细胞。tpm量化表皮黑色素含量与MI呈正相关,与ITA呈负相关。rcm检测到的皮肤炎症细胞在活性黄褐斑中比在稳定黄褐斑中更普遍。总之,TPM和RCM协同捕获关键的黄褐斑特征,TPM通过表皮黑色素定量评估疾病严重程度,而RCM通过检测炎症细胞反映疾病活动性。这为临床医生提供了精确管理的量身定制的成像工具。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Noninvasive Assessment of Melasma Pathological Features: Side-By-Side Comparison of Two-Photon Microscopy and Reflectance Confocal Microscopy

Noninvasive Assessment of Melasma Pathological Features: Side-By-Side Comparison of Two-Photon Microscopy and Reflectance Confocal Microscopy

Melasma, a refractory hyperpigmentation disorder, requires noninvasive tools for accurate pathological assessment. This study compared two-photon microscopy (TPM) and reflectance confocal microscopy (RCM) for the in vivo characterization of melasma pathology. In this cross-sectional study, TPM and RCM features and imaging clarity were evaluated in 130 melasma patients. Spearman correlation analyses were performed between TPM-quantified epidermal melanin, the melanin index (MI), and the individual typology angle (ITA). Features were also compared between active and stable disease stages. TPM and RCM showed substantial agreement in detecting increased epidermal melanin (κ = 0.651), activated melanocytes at the dermal-epidermal junction (DEJ) (κ = 0.711), and flattened rete ridges (κ = 0.691) (all p < 0.001). TPM excelled in visualizing intracellular melanin distribution, pendulous melanocytes under the DEJ, and solar elastosis, while RCM better identified activated melanocytes at the DEJ. TPM-quantified epidermal melanin content correlated positively with MI and negatively with ITA. RCM-detected dermal inflammatory cells were more prevalent in active than in stable melasma. In conclusion, TPM and RCM synergistically capture critical melasma features, with TPM assessing disease severity via epidermal melanin quantification, whereas RCM reflects disease activity by detecting inflammatory cells. This provides clinicians with tailored imaging tools for precision management.

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来源期刊
Pigment Cell & Melanoma Research
Pigment Cell & Melanoma Research 医学-皮肤病学
CiteScore
8.90
自引率
2.30%
发文量
54
审稿时长
6-12 weeks
期刊介绍: Pigment Cell & Melanoma Researchpublishes manuscripts on all aspects of pigment cells including development, cell and molecular biology, genetics, diseases of pigment cells including melanoma. Papers that provide insights into the causes and progression of melanoma including the process of metastasis and invasion, proliferation, senescence, apoptosis or gene regulation are especially welcome, as are papers that use the melanocyte system to answer questions of general biological relevance. Papers that are purely descriptive or make only minor advances to our knowledge of pigment cells or melanoma in particular are not suitable for this journal. Keywords Pigment Cell & Melanoma Research, cell biology, melatonin, biochemistry, chemistry, comparative biology, dermatology, developmental biology, genetics, hormones, intracellular signalling, melanoma, molecular biology, ocular and extracutaneous melanin, pharmacology, photobiology, physics, pigmentary disorders
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