用于鉴别混杂酰胺水解酶的活性探针文库

IF 4.2 2区化学 Q2 CHEMISTRY, MULTIDISCIPLINARY

Chemical Communications Pub Date : 2025-10-01 DOI:10.1039/d5cc04162g

Chathuri Kombala, Jared Kroll, Lucas Webber, Stephen J Callister, Natalie C Sadler, Erin Bredeweg, Irlanda Medrano, Kristoffer R Brandvold, Sankarganesh Krishnamoorthy

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引用次数: 0

摘要

建立了一个荧光底物文库，用于检测土壤来源的几丁质降解细菌的酰胺水解酶活性。Hit化合物被转化为下拉探针，用于化学蛋白质组学富集，鉴定以前未注释的蛋白质，现在与酰胺水解相关。这种方法可以发现新的酰胺水解酶，在环境，生物制造和医学方面具有潜在的应用。

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An activity-based probe library for identifying promiscuous amide hydrolases

A fluorogenic substrate library was developed to detect amide hydrolase activity in soil-derived chitin-degrading bacteria. Hit compounds were converted into pull-down probes for chemoproteomic enrichment, identifying previously unannotated proteins now linked to amide hydrolysis. This approach enables discovery of novel amide hydrolases with potential applications in environment, biomanufacturing, and medicine.

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来源期刊

Chemical Communications 化学-化学综合

CiteScore

8.60

自引率

4.10%

发文量

2705

审稿时长

1.4 months

期刊介绍： ChemComm (Chemical Communications) is renowned as the fastest publisher of articles providing information on new avenues of research, drawn from all the world''s major areas of chemical research.