Sasmita Behera, Soumya Priyadarsinee Mohanty, C. Anilkumar, Muhammed Azharudheen T.P., Debashis Moharana, B.C. Marndi, Kutubuddin Ali Molla, A. Anandan, Anjani kumar, Gowda G. Basana, R. Beena, S. Raghu, Lalan Kumar Singh, Rameswar Prasad Sah
{"title":"水稻直接播种适应相关快速均匀萌发性状的全基因组关联图谱","authors":"Sasmita Behera, Soumya Priyadarsinee Mohanty, C. Anilkumar, Muhammed Azharudheen T.P., Debashis Moharana, B.C. Marndi, Kutubuddin Ali Molla, A. Anandan, Anjani kumar, Gowda G. Basana, R. Beena, S. Raghu, Lalan Kumar Singh, Rameswar Prasad Sah","doi":"10.1017/s0960258525100032","DOIUrl":null,"url":null,"abstract":"<p>The genetic basis of rapid and uniform seed germination and its associated traits is crucial for improving seed vigour and seedling establishment for higher productivity in direct-seeded rice (DSR) systems. This study investigates the phenotypic diversity and genetic architecture of germination traits in 163 rice genotypes, using a genome-wide association studies (GWAS). An association panel of 163 diverse rice genotypes, including varieties, germplasm and breeding lines, was evaluated for seed germination traits over 2 years (2022 and 2023). The panel was genotyped using 295 simple sequence repeat (SSR) markers, including 80 random SSRs and 215 candidate gene SSRs linked to seed traits and morphological attributes. The genotyping of 163 lines with 295 markers revealed a range of genetic diversity, with polymorphic information content values between 0.04 and 0.93. Population structure analysis indicated the presence of two groups and four sub-groups. GWAS identified 80 significant marker-trait associations (MTAs) across 12 chromosomes at <span>P</span> ≤ 0.05, which narrow down to 18 MTAs at <span>P</span> ≤ 0.01. Twelve candidate genes are identified which were related with multiple traits, linked to important functions, such as seed-size regulation, nutrient mobilization and plant growth. Candidate gene-based SSR (cgSSR) markers such as M169 (OsMIK), M57 (THIS1), M66 (GW2), and M18 (OsBAK1), displayed pleiotropy including rapid and uniform germination (germination index, germination rate index and mean germination time) traits. The newly identified candidate gene markers associated with seed rapid and uniform germination traits can be leveraged in marker-assisted breeding programs to introduce diverse alleles for enhanced seed vigour and crop establishment. Markers closely linked to multiple traits hold significant potential for the simultaneous improvement of several traits.</p>","PeriodicalId":21711,"journal":{"name":"Seed Science Research","volume":"72 1","pages":""},"PeriodicalIF":1.9000,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Genome-wide association mapping for rapid and uniform germination traits associated with direct-seeded adaptation in rice\",\"authors\":\"Sasmita Behera, Soumya Priyadarsinee Mohanty, C. Anilkumar, Muhammed Azharudheen T.P., Debashis Moharana, B.C. Marndi, Kutubuddin Ali Molla, A. Anandan, Anjani kumar, Gowda G. Basana, R. Beena, S. Raghu, Lalan Kumar Singh, Rameswar Prasad Sah\",\"doi\":\"10.1017/s0960258525100032\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>The genetic basis of rapid and uniform seed germination and its associated traits is crucial for improving seed vigour and seedling establishment for higher productivity in direct-seeded rice (DSR) systems. This study investigates the phenotypic diversity and genetic architecture of germination traits in 163 rice genotypes, using a genome-wide association studies (GWAS). An association panel of 163 diverse rice genotypes, including varieties, germplasm and breeding lines, was evaluated for seed germination traits over 2 years (2022 and 2023). The panel was genotyped using 295 simple sequence repeat (SSR) markers, including 80 random SSRs and 215 candidate gene SSRs linked to seed traits and morphological attributes. The genotyping of 163 lines with 295 markers revealed a range of genetic diversity, with polymorphic information content values between 0.04 and 0.93. Population structure analysis indicated the presence of two groups and four sub-groups. GWAS identified 80 significant marker-trait associations (MTAs) across 12 chromosomes at <span>P</span> ≤ 0.05, which narrow down to 18 MTAs at <span>P</span> ≤ 0.01. Twelve candidate genes are identified which were related with multiple traits, linked to important functions, such as seed-size regulation, nutrient mobilization and plant growth. Candidate gene-based SSR (cgSSR) markers such as M169 (OsMIK), M57 (THIS1), M66 (GW2), and M18 (OsBAK1), displayed pleiotropy including rapid and uniform germination (germination index, germination rate index and mean germination time) traits. The newly identified candidate gene markers associated with seed rapid and uniform germination traits can be leveraged in marker-assisted breeding programs to introduce diverse alleles for enhanced seed vigour and crop establishment. 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Genome-wide association mapping for rapid and uniform germination traits associated with direct-seeded adaptation in rice
The genetic basis of rapid and uniform seed germination and its associated traits is crucial for improving seed vigour and seedling establishment for higher productivity in direct-seeded rice (DSR) systems. This study investigates the phenotypic diversity and genetic architecture of germination traits in 163 rice genotypes, using a genome-wide association studies (GWAS). An association panel of 163 diverse rice genotypes, including varieties, germplasm and breeding lines, was evaluated for seed germination traits over 2 years (2022 and 2023). The panel was genotyped using 295 simple sequence repeat (SSR) markers, including 80 random SSRs and 215 candidate gene SSRs linked to seed traits and morphological attributes. The genotyping of 163 lines with 295 markers revealed a range of genetic diversity, with polymorphic information content values between 0.04 and 0.93. Population structure analysis indicated the presence of two groups and four sub-groups. GWAS identified 80 significant marker-trait associations (MTAs) across 12 chromosomes at P ≤ 0.05, which narrow down to 18 MTAs at P ≤ 0.01. Twelve candidate genes are identified which were related with multiple traits, linked to important functions, such as seed-size regulation, nutrient mobilization and plant growth. Candidate gene-based SSR (cgSSR) markers such as M169 (OsMIK), M57 (THIS1), M66 (GW2), and M18 (OsBAK1), displayed pleiotropy including rapid and uniform germination (germination index, germination rate index and mean germination time) traits. The newly identified candidate gene markers associated with seed rapid and uniform germination traits can be leveraged in marker-assisted breeding programs to introduce diverse alleles for enhanced seed vigour and crop establishment. Markers closely linked to multiple traits hold significant potential for the simultaneous improvement of several traits.
期刊介绍:
Seed Science Research, the official journal of the International Society for Seed Science, is a leading international journal featuring high-quality original papers and review articles on the fundamental aspects of seed science, reviewed by internationally distinguished editors. The emphasis is on the physiology, biochemistry, molecular biology and ecology of seeds.