水稻直接播种适应相关快速均匀萌发性状的全基因组关联图谱

IF 1.9 3区 生物学 Q2 PLANT SCIENCES
Sasmita Behera, Soumya Priyadarsinee Mohanty, C. Anilkumar, Muhammed Azharudheen T.P., Debashis Moharana, B.C. Marndi, Kutubuddin Ali Molla, A. Anandan, Anjani kumar, Gowda G. Basana, R. Beena, S. Raghu, Lalan Kumar Singh, Rameswar Prasad Sah
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引用次数: 0

摘要

种子快速均匀萌发及其相关性状的遗传基础对提高种子活力和种子成苗以提高直接播种水稻(DSR)系统的生产力至关重要。本研究利用全基因组关联研究(GWAS)对163个水稻基因型的萌发性状的表型多样性和遗传结构进行了研究。一个由163种不同水稻基因型组成的协会小组,包括品种、种质和育种系,在2年(2022年和2023年)期间对种子萌发特性进行了评估。利用295个SSR标记,包括80个随机SSR标记和215个与种子性状和形态性状相关的候选基因SSR标记,对该群体进行基因分型。163个品系295个标记的基因分型显示了一定的遗传多样性,多态性信息含量值在0.04 ~ 0.93之间。种群结构分析显示存在2个类群和4个亚类群。GWAS在12条染色体上鉴定出80个显著标记-性状关联(mta) (P≤0.05),在P≤0.01的水平上进一步缩小到18个显著标记-性状关联。12个候选基因与多种性状相关,与种子大小调节、养分动员和植物生长等重要功能相关。候选基因SSR (cgSSR)标记M169 (OsMIK)、M57 (THIS1)、M66 (GW2)和M18 (OsBAK1)表现出快速均匀萌发(萌发指数、发芽率指数和平均萌发时间)等性状的多效性。新发现的候选基因标记与种子快速和均匀发芽性状相关,可以在标记辅助育种计划中利用,引入不同的等位基因,以提高种子活力和作物建立。与多个性状密切相关的标记对多个性状的同时改良具有显著的潜力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Genome-wide association mapping for rapid and uniform germination traits associated with direct-seeded adaptation in rice

The genetic basis of rapid and uniform seed germination and its associated traits is crucial for improving seed vigour and seedling establishment for higher productivity in direct-seeded rice (DSR) systems. This study investigates the phenotypic diversity and genetic architecture of germination traits in 163 rice genotypes, using a genome-wide association studies (GWAS). An association panel of 163 diverse rice genotypes, including varieties, germplasm and breeding lines, was evaluated for seed germination traits over 2 years (2022 and 2023). The panel was genotyped using 295 simple sequence repeat (SSR) markers, including 80 random SSRs and 215 candidate gene SSRs linked to seed traits and morphological attributes. The genotyping of 163 lines with 295 markers revealed a range of genetic diversity, with polymorphic information content values between 0.04 and 0.93. Population structure analysis indicated the presence of two groups and four sub-groups. GWAS identified 80 significant marker-trait associations (MTAs) across 12 chromosomes at P ≤ 0.05, which narrow down to 18 MTAs at P ≤ 0.01. Twelve candidate genes are identified which were related with multiple traits, linked to important functions, such as seed-size regulation, nutrient mobilization and plant growth. Candidate gene-based SSR (cgSSR) markers such as M169 (OsMIK), M57 (THIS1), M66 (GW2), and M18 (OsBAK1), displayed pleiotropy including rapid and uniform germination (germination index, germination rate index and mean germination time) traits. The newly identified candidate gene markers associated with seed rapid and uniform germination traits can be leveraged in marker-assisted breeding programs to introduce diverse alleles for enhanced seed vigour and crop establishment. Markers closely linked to multiple traits hold significant potential for the simultaneous improvement of several traits.

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来源期刊
Seed Science Research
Seed Science Research 生物-植物科学
CiteScore
3.60
自引率
4.80%
发文量
23
审稿时长
>12 weeks
期刊介绍: Seed Science Research, the official journal of the International Society for Seed Science, is a leading international journal featuring high-quality original papers and review articles on the fundamental aspects of seed science, reviewed by internationally distinguished editors. The emphasis is on the physiology, biochemistry, molecular biology and ecology of seeds.
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