Pengcheng Yin, Zhiyang An, Jie Han, Rubo Zhang, Jing Jing, Xiaoling Zhang
{"title":"线粒体靶向I/II型自我报告光敏剂,具有黏度敏感特性,用于肿瘤识别和细胞凋亡诱导","authors":"Pengcheng Yin, Zhiyang An, Jie Han, Rubo Zhang, Jing Jing, Xiaoling Zhang","doi":"10.1016/j.snb.2025.138846","DOIUrl":null,"url":null,"abstract":"Establishing a dynamic correlation between photodynamic therapy (PDT) processes and changes in mitochondrial viscosity is of significant research value for advancing the development of theranostics technologies for tumors. Nevertheless, research on constructing mitochondrial viscosity-sensitive probes at the single-molecule scale that simultaneously possess high type I/II PDT efficacy for tumors and the ability to visualize therapeutic effects remains lacking. In this work, we developed a novel D-π-A type organic small molecule photosensitizer (<strong>TKC)</strong>, constructed by substituting carbazole with tetraphenylethylene (TPE) as the electron-donating moiety (D) and employing indole iodide salt as the electron-accepting component (A). <strong>TKC</strong> is capable of performing highly sensitive and specific fluorescence imaging over a wide viscosity range (η, 1.005-1410<!-- --> <!-- -->cP), enabling real-time in situ imaging of intracellular mitochondrial viscosity as well as accurate differentiation between normal and cancer cells. Furthermore, <strong>TKC</strong> exhibits efficient type I/II ROS generation capabilities, with a <sup>1</sup>O<sub>2</sub> quantum yield of 3.56, while achieving efficient phototoxicity to tumor cells and inhibition of tumor growth in tumor-bearing mice. More importantly, <strong>TKC</strong> can monitor the mitochondrial depolarization process and the dynamic changes of mitochondrial viscosity during the process of apoptosis, enabling real-time tracking and self-reporting of treatment effects. Therefore, the fluorescent probe developed in this work, which integrates precise tumor diagnosis, mitochondrial-targeted therapy and dynamic efficacy evaluation, offers an effective strategy for the subsequent advancement of integrated diagnostic and therapeutic fluorescent tools.","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"113 1","pages":""},"PeriodicalIF":3.7000,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Mitochondrial-targeted type I/II self-reporting photosensitizer with viscosity-sensitive properties for tumor recognition and apoptosis induction\",\"authors\":\"Pengcheng Yin, Zhiyang An, Jie Han, Rubo Zhang, Jing Jing, Xiaoling Zhang\",\"doi\":\"10.1016/j.snb.2025.138846\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Establishing a dynamic correlation between photodynamic therapy (PDT) processes and changes in mitochondrial viscosity is of significant research value for advancing the development of theranostics technologies for tumors. Nevertheless, research on constructing mitochondrial viscosity-sensitive probes at the single-molecule scale that simultaneously possess high type I/II PDT efficacy for tumors and the ability to visualize therapeutic effects remains lacking. In this work, we developed a novel D-π-A type organic small molecule photosensitizer (<strong>TKC)</strong>, constructed by substituting carbazole with tetraphenylethylene (TPE) as the electron-donating moiety (D) and employing indole iodide salt as the electron-accepting component (A). <strong>TKC</strong> is capable of performing highly sensitive and specific fluorescence imaging over a wide viscosity range (η, 1.005-1410<!-- --> <!-- -->cP), enabling real-time in situ imaging of intracellular mitochondrial viscosity as well as accurate differentiation between normal and cancer cells. Furthermore, <strong>TKC</strong> exhibits efficient type I/II ROS generation capabilities, with a <sup>1</sup>O<sub>2</sub> quantum yield of 3.56, while achieving efficient phototoxicity to tumor cells and inhibition of tumor growth in tumor-bearing mice. More importantly, <strong>TKC</strong> can monitor the mitochondrial depolarization process and the dynamic changes of mitochondrial viscosity during the process of apoptosis, enabling real-time tracking and self-reporting of treatment effects. Therefore, the fluorescent probe developed in this work, which integrates precise tumor diagnosis, mitochondrial-targeted therapy and dynamic efficacy evaluation, offers an effective strategy for the subsequent advancement of integrated diagnostic and therapeutic fluorescent tools.\",\"PeriodicalId\":425,\"journal\":{\"name\":\"Sensors and Actuators B: Chemical\",\"volume\":\"113 1\",\"pages\":\"\"},\"PeriodicalIF\":3.7000,\"publicationDate\":\"2025-09-30\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Sensors and Actuators B: Chemical\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://doi.org/10.1016/j.snb.2025.138846\",\"RegionNum\":1,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"CHEMISTRY, ANALYTICAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Sensors and Actuators B: Chemical","FirstCategoryId":"92","ListUrlMain":"https://doi.org/10.1016/j.snb.2025.138846","RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}
Mitochondrial-targeted type I/II self-reporting photosensitizer with viscosity-sensitive properties for tumor recognition and apoptosis induction
Establishing a dynamic correlation between photodynamic therapy (PDT) processes and changes in mitochondrial viscosity is of significant research value for advancing the development of theranostics technologies for tumors. Nevertheless, research on constructing mitochondrial viscosity-sensitive probes at the single-molecule scale that simultaneously possess high type I/II PDT efficacy for tumors and the ability to visualize therapeutic effects remains lacking. In this work, we developed a novel D-π-A type organic small molecule photosensitizer (TKC), constructed by substituting carbazole with tetraphenylethylene (TPE) as the electron-donating moiety (D) and employing indole iodide salt as the electron-accepting component (A). TKC is capable of performing highly sensitive and specific fluorescence imaging over a wide viscosity range (η, 1.005-1410 cP), enabling real-time in situ imaging of intracellular mitochondrial viscosity as well as accurate differentiation between normal and cancer cells. Furthermore, TKC exhibits efficient type I/II ROS generation capabilities, with a 1O2 quantum yield of 3.56, while achieving efficient phototoxicity to tumor cells and inhibition of tumor growth in tumor-bearing mice. More importantly, TKC can monitor the mitochondrial depolarization process and the dynamic changes of mitochondrial viscosity during the process of apoptosis, enabling real-time tracking and self-reporting of treatment effects. Therefore, the fluorescent probe developed in this work, which integrates precise tumor diagnosis, mitochondrial-targeted therapy and dynamic efficacy evaluation, offers an effective strategy for the subsequent advancement of integrated diagnostic and therapeutic fluorescent tools.
期刊介绍:
Sensors & Actuators, B: Chemical is an international journal focused on the research and development of chemical transducers. It covers chemical sensors and biosensors, chemical actuators, and analytical microsystems. The journal is interdisciplinary, aiming to publish original works showcasing substantial advancements beyond the current state of the art in these fields, with practical applicability to solving meaningful analytical problems. Review articles are accepted by invitation from an Editor of the journal.