{"title":"蜜蜂营养转录组学揭示了精确营养的关键见解","authors":"Alexander McMenamin, Vincent Ricigliano","doi":"10.1016/j.jare.2025.09.050","DOIUrl":null,"url":null,"abstract":"<h3>Introduction</h3>Honey bees are essential managed pollinators faced by nutritional deficiencies which contributes to worldwide colony losses. The integration of nutrition science with genomics offers a powerful approach to optimize honey bee health by augmenting immune function and resilience to stressors through precision diet interventions.<h3>Objectives</h3>To uncover the impact of honey bee genetics on core physiological responses to natural and artificial diets.<h3>Methods</h3>Two honey bee genetic backgrounds (Russian and Pol-line) were fed diets consisting of sugar, pollen, and microalgae biomass from either <em>Chlorella vulgaris</em> or spirulina. Nutrigenetic variation was assessed by body weight measurements followed by transcriptome sequencing. Standard differential gene expression analysis and supervised machine learning algorithms were used to measure genotype-specific transcriptional responses to the diets.<h3>Results</h3>Known (i.e., <em>vitellogenin</em> and <em>Jhe, hex70a</em>) and novel (i.e., <em>Glob1, slif</em> and <em>sad</em>) biomarkers of nutritional health exhibited a significant genetics-by-diet interaction. Furthermore, Russian bees featured a contracted transcriptional response, and a transcriptomic signature of delayed behavioral maturation as compared to Pol-line bees, suggesting alternative nutrient assimilation strategies between the two genetic backgrounds. Regardless of diet, Russian bees had significantly lower abundance of a major viral pathogen (DWV). Lastly, higher expression of humoral and cellular immune genes was detected in microalgae-fed bees, though spirulina was significantly more immunogenic than <em>Chlorella</em>.<h3>Conclusion</h3>Genetic background plays a key role in shaping honey bee responses to nutrition. By identifying novel biomarkers of nutritional status, our results support the unique benefits of two microalgae species as functional feed additives. Furthermore, these results reveal the importance of genetics as a consideration when optimizing supplemental feed for an agricultural pollinator. These findings contribute towards the development of tailored nutrition strategies and artificial diets to improve honey bee health.","PeriodicalId":14952,"journal":{"name":"Journal of Advanced Research","volume":"65 1","pages":""},"PeriodicalIF":13.0000,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Honey bee nutritranscriptomics reveals key insights towards precision nutrition\",\"authors\":\"Alexander McMenamin, Vincent Ricigliano\",\"doi\":\"10.1016/j.jare.2025.09.050\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<h3>Introduction</h3>Honey bees are essential managed pollinators faced by nutritional deficiencies which contributes to worldwide colony losses. The integration of nutrition science with genomics offers a powerful approach to optimize honey bee health by augmenting immune function and resilience to stressors through precision diet interventions.<h3>Objectives</h3>To uncover the impact of honey bee genetics on core physiological responses to natural and artificial diets.<h3>Methods</h3>Two honey bee genetic backgrounds (Russian and Pol-line) were fed diets consisting of sugar, pollen, and microalgae biomass from either <em>Chlorella vulgaris</em> or spirulina. Nutrigenetic variation was assessed by body weight measurements followed by transcriptome sequencing. Standard differential gene expression analysis and supervised machine learning algorithms were used to measure genotype-specific transcriptional responses to the diets.<h3>Results</h3>Known (i.e., <em>vitellogenin</em> and <em>Jhe, hex70a</em>) and novel (i.e., <em>Glob1, slif</em> and <em>sad</em>) biomarkers of nutritional health exhibited a significant genetics-by-diet interaction. Furthermore, Russian bees featured a contracted transcriptional response, and a transcriptomic signature of delayed behavioral maturation as compared to Pol-line bees, suggesting alternative nutrient assimilation strategies between the two genetic backgrounds. Regardless of diet, Russian bees had significantly lower abundance of a major viral pathogen (DWV). Lastly, higher expression of humoral and cellular immune genes was detected in microalgae-fed bees, though spirulina was significantly more immunogenic than <em>Chlorella</em>.<h3>Conclusion</h3>Genetic background plays a key role in shaping honey bee responses to nutrition. By identifying novel biomarkers of nutritional status, our results support the unique benefits of two microalgae species as functional feed additives. Furthermore, these results reveal the importance of genetics as a consideration when optimizing supplemental feed for an agricultural pollinator. These findings contribute towards the development of tailored nutrition strategies and artificial diets to improve honey bee health.\",\"PeriodicalId\":14952,\"journal\":{\"name\":\"Journal of Advanced Research\",\"volume\":\"65 1\",\"pages\":\"\"},\"PeriodicalIF\":13.0000,\"publicationDate\":\"2025-09-30\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Advanced Research\",\"FirstCategoryId\":\"103\",\"ListUrlMain\":\"https://doi.org/10.1016/j.jare.2025.09.050\",\"RegionNum\":1,\"RegionCategory\":\"综合性期刊\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"MULTIDISCIPLINARY SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Advanced Research","FirstCategoryId":"103","ListUrlMain":"https://doi.org/10.1016/j.jare.2025.09.050","RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"MULTIDISCIPLINARY SCIENCES","Score":null,"Total":0}
Honey bee nutritranscriptomics reveals key insights towards precision nutrition
Introduction
Honey bees are essential managed pollinators faced by nutritional deficiencies which contributes to worldwide colony losses. The integration of nutrition science with genomics offers a powerful approach to optimize honey bee health by augmenting immune function and resilience to stressors through precision diet interventions.
Objectives
To uncover the impact of honey bee genetics on core physiological responses to natural and artificial diets.
Methods
Two honey bee genetic backgrounds (Russian and Pol-line) were fed diets consisting of sugar, pollen, and microalgae biomass from either Chlorella vulgaris or spirulina. Nutrigenetic variation was assessed by body weight measurements followed by transcriptome sequencing. Standard differential gene expression analysis and supervised machine learning algorithms were used to measure genotype-specific transcriptional responses to the diets.
Results
Known (i.e., vitellogenin and Jhe, hex70a) and novel (i.e., Glob1, slif and sad) biomarkers of nutritional health exhibited a significant genetics-by-diet interaction. Furthermore, Russian bees featured a contracted transcriptional response, and a transcriptomic signature of delayed behavioral maturation as compared to Pol-line bees, suggesting alternative nutrient assimilation strategies between the two genetic backgrounds. Regardless of diet, Russian bees had significantly lower abundance of a major viral pathogen (DWV). Lastly, higher expression of humoral and cellular immune genes was detected in microalgae-fed bees, though spirulina was significantly more immunogenic than Chlorella.
Conclusion
Genetic background plays a key role in shaping honey bee responses to nutrition. By identifying novel biomarkers of nutritional status, our results support the unique benefits of two microalgae species as functional feed additives. Furthermore, these results reveal the importance of genetics as a consideration when optimizing supplemental feed for an agricultural pollinator. These findings contribute towards the development of tailored nutrition strategies and artificial diets to improve honey bee health.
期刊介绍:
Journal of Advanced Research (J. Adv. Res.) is an applied/natural sciences, peer-reviewed journal that focuses on interdisciplinary research. The journal aims to contribute to applied research and knowledge worldwide through the publication of original and high-quality research articles in the fields of Medicine, Pharmaceutical Sciences, Dentistry, Physical Therapy, Veterinary Medicine, and Basic and Biological Sciences.
The following abstracting and indexing services cover the Journal of Advanced Research: PubMed/Medline, Essential Science Indicators, Web of Science, Scopus, PubMed Central, PubMed, Science Citation Index Expanded, Directory of Open Access Journals (DOAJ), and INSPEC.