H3乙酰化触发SWR1募集，促进H2A。与染色质结合并刺激基因表达。

IF 6.9 1区生物学 Q1 PLANT SCIENCES

Plant Physiology Pub Date : 2025-09-30 DOI:10.1093/plphys/kiaf469

Han Jiang,Mingming Zhao,Min Liu,Wenshuo Zhang,Junjie Xu,Thierry Pélissier,Olivier Mathieu,Israel Ausin,Yafei Wang

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引用次数: 0

摘要

在DNA复制之后，真核生物中典型的H2A经常被组蛋白变体所取代，例如保守的H2A. z。染色质重塑因子如SWI/SNF-Related 1 （SWR1）复合体促进了这一过程，用H2A取代H2A组蛋白。Z以atp依赖的方式。然而，将SWR1招募到特定位点的确切机制仍然难以捉摸。在本研究中，我们探讨了H3乙酰化在介导H2A中的作用。与染色质结合促进基因表达。我们的研究结果表明，人工诱导的超乙酰化与较高水平的H2A有关。Z和H2A的减少。W （HTA6和HTA7）。在组蛋白去乙酰化酶6 （HDA6）和组蛋白去乙酰化酶9 （HDA9）缺陷的遗传背景中也观察到这种现象。此外,H2A。在hda6-1和hda9-1中观察到的表型需要Z。

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H3 acetylation triggers SWR1 recruitment to promote H2A.Z incorporation into chromatin and stimulate gene expression.

Following DNA replication, the canonical H2A in eukaryotes is frequently replaced by histone variants, such as the conserved H2A.Z. Chromatin remodelers like the SWI/SNF-Related 1 (SWR1) complex facilitate this process, substituting the H2A histone with H2A.Z in an ATP-dependent manner. However, the precise mechanisms that recruit SWR1 to specific loci remain elusive. In this study, we investigate the role of H3 acetylation in mediating H2A.Z incorporation into chromatin to promote gene expression. Our results show that artificially induced hyperacetylation is associated with higher levels of H2A.Z and a decrease in H2A.W (HTA6 and HTA7). This phenomenon is also observed in genetic backgrounds that are defective in the histone deacetylases HISTONE DEACETYLASE 6 (HDA6) and HISTONE DEACETYLASE 9 (HDA9). Moreover, H2A.Z is required for the phenotypes observed in hda6-1 and hda9-1.

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来源期刊

Plant Physiology 生物-植物科学

CiteScore

12.20

自引率

5.40%

发文量

535

审稿时长

2.3 months

期刊介绍： Plant Physiology® is a distinguished and highly respected journal with a rich history dating back to its establishment in 1926. It stands as a leading international publication in the field of plant biology, covering a comprehensive range of topics from the molecular and structural aspects of plant life to systems biology and ecophysiology. Recognized as the most highly cited journal in plant sciences, Plant Physiology® is a testament to its commitment to excellence and the dissemination of groundbreaking research. As the official publication of the American Society of Plant Biologists, Plant Physiology® upholds rigorous peer-review standards, ensuring that the scientific community receives the highest quality research. The journal releases 12 issues annually, providing a steady stream of new findings and insights to its readership.