炭疽菌CGMCC 3.17371株pyrecinine A的异源表达。

IF 1.6 3区化学 Q3 CHEMISTRY, APPLIED

Natural Product Research Pub Date : 2025-09-29 DOI:10.1080/14786419.2025.2568206

Chao Chen, Yuxin Shi, Haizhifei Ding, Lina Yu, Wei Lin, Jianbin Xiao, Huaidong Zhang, Qin Li, Mingliang Zhang, Li Li

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引用次数: 0

摘要

吡咯诺碱A是一种吡喃酮衍生物，具有抗病毒、抗菌和抗癌活性。通过基因组挖掘，我们成功鉴定了炭疽菌CGMCC 3.17371生物合成吡咯诺碱A的基因簇。通过异源表达分离得到6个化合物，阐明了芘甲素在体内的生物合成途径。该基因簇的鉴定可能为代谢工程方法优化吡咯诺辛a的生产提供平台。值得注意的是，生物学评价显示，吡诺酚A及其衍生物在体外对三种人类癌细胞系（DLD-1、U-87MG和U251）具有强大的细胞毒性。这些发现有力地表明它们作为新型抗癌药物的治疗潜力，特别是对于对常规治疗有耐药性的恶性肿瘤。

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Heterologous expression of Pyrenocine A from Colletotrichum gloeosporioides CGMCC 3.17371.

Pyrenocine A is a pyranone derivative known for its antiviral, antibacterial, and anti-cancer cell proliferation activities. Through genomic mining, we successfully identified the gene cluster responsible for the biosynthesis of pyrenocine A in Colletotrichum gloeosporioides CGMCC 3.17371. Six compounds were isolated through heterologous expression, which allowed us to elucidate the biosynthetic pathway of pyrenocine A in vivo. The identification of this gene cluster may provide a platform for metabolic engineering approaches to optimise pyrenocine A production. Notably, biological evaluations revealed that pyrenocine A and its derivative exhibited potent cytotoxicity against three human cancer cell lines (DLD-1, U-87MG, and U251) in vitro. These findings strongly suggest their therapeutic potential as novel anticancer agents, particularly for malignancies that demonstrate resistance to conventional treatments.

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来源期刊

Natural Product Research 化学-医药化学

CiteScore

5.10

自引率

9.10%

发文量

605

审稿时长

2.1 months

期刊介绍： The aim of Natural Product Research is to publish important contributions in the field of natural product chemistry. The journal covers all aspects of research in the chemistry and biochemistry of naturally occurring compounds. The communications include coverage of work on natural substances of land and sea and of plants, microbes and animals. Discussions of structure elucidation, synthesis and experimental biosynthesis of natural products as well as developments of methods in these areas are welcomed in the journal. Finally, research papers in fields on the chemistry-biology boundary, eg. fermentation chemistry, plant tissue culture investigations etc., are accepted into the journal. Natural Product Research issues will be subtitled either ""Part A - Synthesis and Structure"" or ""Part B - Bioactive Natural Products"". for details on this , see the forthcoming articles section. All manuscript submissions are subject to initial appraisal by the Editor, and, if found suitable for further consideration, to peer review by independent, anonymous expert referees. All peer review is single blind and submission is online via ScholarOne Manuscripts.