使用含或不含生物活性玻璃的氟化物牙膏逆转牙根龋——一项体外研究。

IF 5.5 2区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of dentistry Pub Date : 2025-09-27 DOI:10.1016/j.jdent.2025.106139

Haoran Chen, Robert Hill, Aylin Baysan

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引用次数: 0

摘要

目的：评价含生物活性玻璃和不含生物活性玻璃的含氟牙膏对牙根龋的治疗效果。材料和方法：对94颗拔牙128颗牙根龋进行基线和3个月后的视觉-触觉评估和光致荧光评估仪（SoproLIFE）评估，以确定病变的严重程度。每个病变随机分为四组：组1：含540ppm氟化物的生物活性玻璃（BG），组2：含5000ppm氟化物，组3：含1450ppm氟化物，组4：去离子水。每天进行两次标准化刷牙。样品保存在再矿化溶液（pH7）中，温度37℃。3个月时进行拉曼光谱和扫描电镜检查。结果：治疗组大鼠牙根龋严重程度指数均显著降低（p=0.03）。第2组44%的龋变硬（SI0），第1组41%，第3组38%，第4组19%的龋变硬（SI0）。SoproLIFE的结果表明，在含氟540ppm的BG组中，25%的病变变硬，在含氟5000ppm组中，19%的病变变硬，在含氟1450ppm组中，16%的病变变硬，在去离子水组中，28%的病变变硬。仅去离子水组病变加重3%。拉曼光谱显示，磷酸盐含量在5000ppm氟中最高，其次是BG，含540ppm氟bbb、1450ppm氟>去离子水基团。扫描电镜分析显示，所有牙膏组的牙本质小管都是部分或完全封闭的，而去离子水组的牙本质小管是暴露的。结论：在本研究的限制范围内，所有牙膏都可能有效地硬化根龋病变和再矿化。5000ppm氟化物在促进再矿化方面有较好的效果。临床意义：所有研究表明牙膏在阻止和逆转牙根龋方面都有潜在的益处。这些发现可以提供进一步的证据，支持制定更新的牙根龋指南。

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Reversal of root caries using fluoride toothpastes with or without bioactive glass - an in vitro study

Objectives

To evaluate the effect of fluoridated toothpastes with or without bioactive glass on root caries.

Materials and Methods

A total of 94 extracted teeth with 128 root caries were assessed using visual-tactile assessments and light-induced fluorescence-evaluator (SoproLIFE) at baseline and after three months to determine the severity of lesions. Each lesion was randomly allocated into four groups: Group-1: Bioactive glass (BG) with 540 ppm fluoride, Group-2: 5,000 ppm fluoride, Group-3: 1,450 ppm fluoride, Group-4: Deionised water. Standardised tooth-brushing was performed twice a day. Samples were stored in remineralising solution (pH7) at 37°C. Raman spectroscopy and scanning electron microscopy (SEM) were conducted at three months.

Results

There was a significant decrease in severity index of root caries in treatment groups (p=0.03). 44% of lesions in Group-2 reversed and became hard (SI0), whilst 41% in Group-1, 38% and 19% of root caries in Groups-3 and 4 respectively reversed to SI0. The SoproLIFE results demonstrated that 25% of lesions in BG with 540 ppm fluoride, 19% in 5,000 ppm fluoride, whilst 16% in 1,450 ppm fluoride, 28% in deionised water group became hard. 3% of lesions became worse in deionised water group only. Raman spectroscopy indicated that phosphate content was highest in 5,000 ppm fluoride, followed by BG with 540 ppm fluoride>1,450 ppm fluoride>deionised water groups. SEM analyses showed partially/completely closed dentinal tubules in all toothpaste groups, however, tubules were exposed in deionised water group.

Conclusion

Within the limitations of this study, all toothpastes were potentially effective in hardening root carious lesions and remineralisation. 5,000 ppm fluoride had a superior effect in promoting remineralisation.

Clinical significance

All study toothpastes have potential benefits in arresting and reversing root caries. These findings can provide further evidence to support the development of updated guidelines for root caries.

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来源期刊

Journal of dentistry 医学-牙科与口腔外科

CiteScore

7.30

自引率

11.40%

发文量

349

审稿时长

35 days

期刊介绍： The Journal of Dentistry has an open access mirror journal The Journal of Dentistry: X, sharing the same aims and scope, editorial team, submission system and rigorous peer review. The Journal of Dentistry is the leading international dental journal within the field of Restorative Dentistry. Placing an emphasis on publishing novel and high-quality research papers, the Journal aims to influence the practice of dentistry at clinician, research, industry and policy-maker level on an international basis. Topics covered include the management of dental disease, periodontology, endodontology, operative dentistry, fixed and removable prosthodontics, dental biomaterials science, long-term clinical trials including epidemiology and oral health, technology transfer of new scientific instrumentation or procedures, as well as clinically relevant oral biology and translational research. The Journal of Dentistry will publish original scientific research papers including short communications. It is also interested in publishing review articles and leaders in themed areas which will be linked to new scientific research. Conference proceedings are also welcome and expressions of interest should be communicated to the Editor.