{"title":"利用超短回波时间MRI对T1DM兔胫骨孔隙度的纵向体内评估。","authors":"Kejun Wang, Weiyin Vivian Liu, Yajun Ma, Zhao Wei, Haoran Lei, Chuanyun Jiang, Minzhi Pei, Yunfei Zha","doi":"10.1002/jmri.70133","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Type 1 diabetes mellitus (T1DM) raises fracture risk. Longitudinal changes in cortical porosity during diabetes progression are unclear.</p><p><strong>Purpose: </strong>To assess, using ultra short echo time (UTE)-MRI for tibia cortical porosity and geometry changes in T1DM rabbit model, using micro-CT (μCT) and bone remodeling biomarkers as reference.</p><p><strong>Study type: </strong>Longitudinal, animal model.</p><p><strong>Animal model: </strong>Twenty-eight male Japanese white rabbits (age: 8-10 weeks, weight: 2.14 ± 0.24 kg) randomized to diabetic (n = 16) and control (n = 12) subgroups.</p><p><strong>Field strength/sequence: </strong>3 T; 3D dual-echo UTE and 3D inversion recovery (IR)-UTE sequences.</p><p><strong>Assessment: </strong>UTE-MRI were performed at baseline and multiple timepoints. Porosity index (PI), suppression ratio (SR), and cortical thickness were assessed accordingly via the signal intensity (SI) at echo time (TE) at 2.2 and 0.032 ms, SI of UTE to IR-UTE of TE at 0.032 ms, and based on geometric modeling. μCT-derived porosity, pore diameter, and cortical thickness were conducted at week 24. Immunohistochemistry and quantitative polymerase chain reaction (qPCR) analyzed bone remodeling markers.</p><p><strong>Statistical tests: </strong>Group comparisons were made using t tests/Mann-Whitney U tests and repeated measures ANOVA. Spearman correlation coefficients (r) assessed the strength of the associations between PI and bone markers. A p value < 0.05 was considered significant.</p><p><strong>Results: </strong>24-week T1DM rabbits had significantly UTE-MRI-measured higher cortical porosity (0.42 ± 0.05 vs. 0.32 ± 0.04) and lower cortical thickness (0.92 ± 0.11 vs. 1.40 ± 0.18) than the control group, consistent with μCT measurements. PI was significantly positively correlated with μCT-measured porosity and pore diameter (r = 0.91, 0.69, respectively), while SR showed no correlation (r = -0.33, p = 0.11; r = -0.25, p = 0.25). PI increased significantly in T1DM rabbits from week 12 compared to the controls (0.35 ± 0.05 vs. 0.31 ± 0.04). The lower osteoblast and osteoclast-related cell numbers and expression were negatively correlated with PI in diabetic rabbits (r = -0.871 to -0.625).</p><p><strong>Data conclusion: </strong>UTE-MRI has the potential to longitudinally monitor and identify early reversible cortical porosity and geometric changes.</p><p><strong>Evidence level: </strong>1.</p><p><strong>Technical efficacy: </strong>Stage 2.</p>","PeriodicalId":16140,"journal":{"name":"Journal of Magnetic Resonance Imaging","volume":" ","pages":""},"PeriodicalIF":3.5000,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Longitudinal In Vivo Assessment of Tibia Bone Porosity in T1DM Rabbits Using Ultrashort Echo Time MRI.\",\"authors\":\"Kejun Wang, Weiyin Vivian Liu, Yajun Ma, Zhao Wei, Haoran Lei, Chuanyun Jiang, Minzhi Pei, Yunfei Zha\",\"doi\":\"10.1002/jmri.70133\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>Type 1 diabetes mellitus (T1DM) raises fracture risk. Longitudinal changes in cortical porosity during diabetes progression are unclear.</p><p><strong>Purpose: </strong>To assess, using ultra short echo time (UTE)-MRI for tibia cortical porosity and geometry changes in T1DM rabbit model, using micro-CT (μCT) and bone remodeling biomarkers as reference.</p><p><strong>Study type: </strong>Longitudinal, animal model.</p><p><strong>Animal model: </strong>Twenty-eight male Japanese white rabbits (age: 8-10 weeks, weight: 2.14 ± 0.24 kg) randomized to diabetic (n = 16) and control (n = 12) subgroups.</p><p><strong>Field strength/sequence: </strong>3 T; 3D dual-echo UTE and 3D inversion recovery (IR)-UTE sequences.</p><p><strong>Assessment: </strong>UTE-MRI were performed at baseline and multiple timepoints. Porosity index (PI), suppression ratio (SR), and cortical thickness were assessed accordingly via the signal intensity (SI) at echo time (TE) at 2.2 and 0.032 ms, SI of UTE to IR-UTE of TE at 0.032 ms, and based on geometric modeling. μCT-derived porosity, pore diameter, and cortical thickness were conducted at week 24. Immunohistochemistry and quantitative polymerase chain reaction (qPCR) analyzed bone remodeling markers.</p><p><strong>Statistical tests: </strong>Group comparisons were made using t tests/Mann-Whitney U tests and repeated measures ANOVA. Spearman correlation coefficients (r) assessed the strength of the associations between PI and bone markers. A p value < 0.05 was considered significant.</p><p><strong>Results: </strong>24-week T1DM rabbits had significantly UTE-MRI-measured higher cortical porosity (0.42 ± 0.05 vs. 0.32 ± 0.04) and lower cortical thickness (0.92 ± 0.11 vs. 1.40 ± 0.18) than the control group, consistent with μCT measurements. PI was significantly positively correlated with μCT-measured porosity and pore diameter (r = 0.91, 0.69, respectively), while SR showed no correlation (r = -0.33, p = 0.11; r = -0.25, p = 0.25). PI increased significantly in T1DM rabbits from week 12 compared to the controls (0.35 ± 0.05 vs. 0.31 ± 0.04). The lower osteoblast and osteoclast-related cell numbers and expression were negatively correlated with PI in diabetic rabbits (r = -0.871 to -0.625).</p><p><strong>Data conclusion: </strong>UTE-MRI has the potential to longitudinally monitor and identify early reversible cortical porosity and geometric changes.</p><p><strong>Evidence level: </strong>1.</p><p><strong>Technical efficacy: </strong>Stage 2.</p>\",\"PeriodicalId\":16140,\"journal\":{\"name\":\"Journal of Magnetic Resonance Imaging\",\"volume\":\" \",\"pages\":\"\"},\"PeriodicalIF\":3.5000,\"publicationDate\":\"2025-09-30\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Magnetic Resonance Imaging\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1002/jmri.70133\",\"RegionNum\":2,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"RADIOLOGY, NUCLEAR MEDICINE & MEDICAL IMAGING\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Magnetic Resonance Imaging","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1002/jmri.70133","RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"RADIOLOGY, NUCLEAR MEDICINE & MEDICAL IMAGING","Score":null,"Total":0}
Longitudinal In Vivo Assessment of Tibia Bone Porosity in T1DM Rabbits Using Ultrashort Echo Time MRI.
Background: Type 1 diabetes mellitus (T1DM) raises fracture risk. Longitudinal changes in cortical porosity during diabetes progression are unclear.
Purpose: To assess, using ultra short echo time (UTE)-MRI for tibia cortical porosity and geometry changes in T1DM rabbit model, using micro-CT (μCT) and bone remodeling biomarkers as reference.
Study type: Longitudinal, animal model.
Animal model: Twenty-eight male Japanese white rabbits (age: 8-10 weeks, weight: 2.14 ± 0.24 kg) randomized to diabetic (n = 16) and control (n = 12) subgroups.
Field strength/sequence: 3 T; 3D dual-echo UTE and 3D inversion recovery (IR)-UTE sequences.
Assessment: UTE-MRI were performed at baseline and multiple timepoints. Porosity index (PI), suppression ratio (SR), and cortical thickness were assessed accordingly via the signal intensity (SI) at echo time (TE) at 2.2 and 0.032 ms, SI of UTE to IR-UTE of TE at 0.032 ms, and based on geometric modeling. μCT-derived porosity, pore diameter, and cortical thickness were conducted at week 24. Immunohistochemistry and quantitative polymerase chain reaction (qPCR) analyzed bone remodeling markers.
Statistical tests: Group comparisons were made using t tests/Mann-Whitney U tests and repeated measures ANOVA. Spearman correlation coefficients (r) assessed the strength of the associations between PI and bone markers. A p value < 0.05 was considered significant.
Results: 24-week T1DM rabbits had significantly UTE-MRI-measured higher cortical porosity (0.42 ± 0.05 vs. 0.32 ± 0.04) and lower cortical thickness (0.92 ± 0.11 vs. 1.40 ± 0.18) than the control group, consistent with μCT measurements. PI was significantly positively correlated with μCT-measured porosity and pore diameter (r = 0.91, 0.69, respectively), while SR showed no correlation (r = -0.33, p = 0.11; r = -0.25, p = 0.25). PI increased significantly in T1DM rabbits from week 12 compared to the controls (0.35 ± 0.05 vs. 0.31 ± 0.04). The lower osteoblast and osteoclast-related cell numbers and expression were negatively correlated with PI in diabetic rabbits (r = -0.871 to -0.625).
Data conclusion: UTE-MRI has the potential to longitudinally monitor and identify early reversible cortical porosity and geometric changes.
期刊介绍：
The Journal of Magnetic Resonance Imaging (JMRI) is an international journal devoted to the timely publication of basic and clinical research, educational and review articles, and other information related to the diagnostic applications of magnetic resonance.
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