Birgit Marlies Govers, Bjorn Bakker, Magdalena J de Bruijne, Arie Oosterhof, Elly Versteeg, Anneke I den Hollander, Sander Keijser, B Jeroen Klevering, Willeke F Daamen, T H van Kuppevelt
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Disaccharide analysis was performed by high-pressure liquid chromatography (HPLC) to assess HA and CS concentrations in the vitreous and determine CS sulfation.</p><p><strong>Results: </strong>The vitreous of RRD patients showed significantly lower HA concentrations (Mann-Whitney U, p<0.001) and higher CS concentrations (Mann-Whitney U, p<0.001) compared to non-RRD patients. The higher total CS concentration in RRD patients was related to a significant increase in non-sulfated CS domains. The concentration of sulfated CS disaccharides with a 6S domain was significantly decreased in RRD patients compared to non-RRD patients: 4S6S (Mann-Whitney U; p=0.008), 2S6S (Mann-Whitney U; p=0.004), and 6S (Mann-Whitney U; p=0.013). The sulfated CS disaccharides 2S4S, 4S6S, 2S6S, 4S, 2S, and 6S, are significantly more abundant in non-RRD patients.</p><p><strong>Conclusions: </strong>The significant degree of non-sulfated CS domains and decreased HA concentrations in the vitreous of RRD patients could weaken structural organization of the vitreous. Reduced vitreous stability can cause pathological vitreoretinal traction, ultimately leading to rhegmatogenous retinal detachment.</p>","PeriodicalId":12177,"journal":{"name":"Experimental eye research","volume":" ","pages":"110672"},"PeriodicalIF":2.7000,"publicationDate":"2025-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Chondroitin sulfate and hyaluronan in the vitreous of patients with rhegmatogenous retinal detachment.\",\"authors\":\"Birgit Marlies Govers, Bjorn Bakker, Magdalena J de Bruijne, Arie Oosterhof, Elly Versteeg, Anneke I den Hollander, Sander Keijser, B Jeroen Klevering, Willeke F Daamen, T H van Kuppevelt\",\"doi\":\"10.1016/j.exer.2025.110672\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Purpose: </strong>To compare hyaluronan (HA) and chondroitin sulfate (CS) concentrations as well as the sulfation pattern of CS domains in the vitreous of rhegmatogenous retinal detachment (RRD) versus non-RRD patients.</p><p><strong>Methods: </strong>Vitreous samples from 56 patients with RRD and 41 patients with floaters, macular hole or epiretinal membranes were collected during pars plana vitrectomy. Disaccharide analysis was performed by high-pressure liquid chromatography (HPLC) to assess HA and CS concentrations in the vitreous and determine CS sulfation.</p><p><strong>Results: </strong>The vitreous of RRD patients showed significantly lower HA concentrations (Mann-Whitney U, p<0.001) and higher CS concentrations (Mann-Whitney U, p<0.001) compared to non-RRD patients. The higher total CS concentration in RRD patients was related to a significant increase in non-sulfated CS domains. The concentration of sulfated CS disaccharides with a 6S domain was significantly decreased in RRD patients compared to non-RRD patients: 4S6S (Mann-Whitney U; p=0.008), 2S6S (Mann-Whitney U; p=0.004), and 6S (Mann-Whitney U; p=0.013). The sulfated CS disaccharides 2S4S, 4S6S, 2S6S, 4S, 2S, and 6S, are significantly more abundant in non-RRD patients.</p><p><strong>Conclusions: </strong>The significant degree of non-sulfated CS domains and decreased HA concentrations in the vitreous of RRD patients could weaken structural organization of the vitreous. Reduced vitreous stability can cause pathological vitreoretinal traction, ultimately leading to rhegmatogenous retinal detachment.</p>\",\"PeriodicalId\":12177,\"journal\":{\"name\":\"Experimental eye research\",\"volume\":\" \",\"pages\":\"110672\"},\"PeriodicalIF\":2.7000,\"publicationDate\":\"2025-09-27\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Experimental eye research\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1016/j.exer.2025.110672\",\"RegionNum\":2,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"OPHTHALMOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Experimental eye research","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1016/j.exer.2025.110672","RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"OPHTHALMOLOGY","Score":null,"Total":0}
引用次数: 0
摘要
目的：比较孔源性视网膜脱离（RRD）和非RRD患者玻璃体中透明质酸（HA）和硫酸软骨素（CS）的浓度以及CS结构域的硫酸化模式。方法：对56例RRD患者和41例有飞蚊、黄斑孔或视网膜前膜的患者行玻璃体切除术，收集玻璃体标本。采用高压液相色谱法测定玻璃体中HA和CS的浓度，并测定CS的磺化程度。结果：RRD患者玻璃体中HA浓度明显降低（Mann-Whitney U, p）。结论：RRD患者玻璃体中非硫酸化CS结构域的显著程度和HA浓度的降低可削弱玻璃体的结构组织。玻璃体稳定性降低可引起病理性玻璃体视网膜牵拉，最终导致孔源性视网膜脱离。
Chondroitin sulfate and hyaluronan in the vitreous of patients with rhegmatogenous retinal detachment.
Purpose: To compare hyaluronan (HA) and chondroitin sulfate (CS) concentrations as well as the sulfation pattern of CS domains in the vitreous of rhegmatogenous retinal detachment (RRD) versus non-RRD patients.
Methods: Vitreous samples from 56 patients with RRD and 41 patients with floaters, macular hole or epiretinal membranes were collected during pars plana vitrectomy. Disaccharide analysis was performed by high-pressure liquid chromatography (HPLC) to assess HA and CS concentrations in the vitreous and determine CS sulfation.
Results: The vitreous of RRD patients showed significantly lower HA concentrations (Mann-Whitney U, p<0.001) and higher CS concentrations (Mann-Whitney U, p<0.001) compared to non-RRD patients. The higher total CS concentration in RRD patients was related to a significant increase in non-sulfated CS domains. The concentration of sulfated CS disaccharides with a 6S domain was significantly decreased in RRD patients compared to non-RRD patients: 4S6S (Mann-Whitney U; p=0.008), 2S6S (Mann-Whitney U; p=0.004), and 6S (Mann-Whitney U; p=0.013). The sulfated CS disaccharides 2S4S, 4S6S, 2S6S, 4S, 2S, and 6S, are significantly more abundant in non-RRD patients.
Conclusions: The significant degree of non-sulfated CS domains and decreased HA concentrations in the vitreous of RRD patients could weaken structural organization of the vitreous. Reduced vitreous stability can cause pathological vitreoretinal traction, ultimately leading to rhegmatogenous retinal detachment.
期刊介绍：
The primary goal of Experimental Eye Research is to publish original research papers on all aspects of experimental biology of the eye and ocular tissues that seek to define the mechanisms of normal function and/or disease. Studies of ocular tissues that encompass the disciplines of cell biology, developmental biology, genetics, molecular biology, physiology, biochemistry, biophysics, immunology or microbiology are most welcomed. Manuscripts that are purely clinical or in a surgical area of ophthalmology are not appropriate for submission to Experimental Eye Research and if received will be returned without review.
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