转录因子MYB通过SSBP2/ISL1轴调控糖酵解,促进胃癌转移和恶性进展。

IF 6 2区 医学 Q1 ONCOLOGY
Zhenni Sun, Mi Zhou, Yasai Yao, Xuehong Chen, Tao Qin, Yafei Han, Lu Yue, Ruyong Yao
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引用次数: 0

摘要

背景:转录因子(TF) MYB对许多生物过程至关重要。单链DNA结合蛋白2 (SSBP2)、胰岛素基因增强蛋白1 (ISL1)和糖酵解参与胃癌(GC)的发生发展。本研究旨在探讨MYB/SSBP2/ISL1轴在胃癌发生发展中的调控机制。方法:利用GC患者癌组织和癌旁组织样本数据集GSE65801筛选差异基因,鉴定GC相关关键tf和tf靶基因,进行表达与生存相关性分析。随后,通过RT-qPCR、免疫组化和Western blot检测GC组织和细胞的表达。CCK-8、克隆、Transwell、伤口愈合实验、流式细胞术和试剂盒检测对细胞的影响。采用EMSA、酵母单杂交、双荧光素酶、ChIP-seq分析和ChIP-PCR分析验证转录因子结合,采用pull-down实验、CO-IP和免疫荧光(IF)验证相互作用。裸鼠皮下肿瘤实验进一步评估MYB的作用。结果:MYB是GC中关键的差异表达TF, SSBP2是MYB的关键靶基因。胃癌患者胃癌组织中MYB表达较高,而SSBP2表达较低。SSBP2是GC细胞中MYB的直接靶点。SSBP2被MYB转录抑制。SSBP2进一步负调控ISL1的表达。MYB敲低可抑制GC细胞的糖酵解、增殖、侵袭和迁移能力,但也可被SSBP2敲低逆转。SSBP2过表达抑制GC细胞的糖酵解、细胞增殖、侵袭和迁移能力,但ISL1过表达可逆转。结论:MYB通过调节SSBP2/ISL1轴介导GC细胞的糖酵解和恶性进展。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Transcription factor MYB regulates glycolysis to promote the transfer and malignant progression of gastric cancer through the SSBP2/ISL1 axis.

Background: The transcription factor (TF) MYB is crucial to many biological processes. Single-stranded DNA binding protein 2 (SSBP2), insulin gene enhancer protein 1 (ISL1) and glycolysis participated in the development of gastric cancer (GC). This study aims to explore the regulatory mechanism of the MYB/SSBP2/ISL1 axis in the development of GC.

Methods: The dataset GSE65801 of cancer tissue and paracancerous tissue samples from patients with GC was used to screen differential genes, identify GC-related key TFs and the target gene of TFs, and perform expression and survival correlation analysis. Subsequently, the expression was verified in GC tissues and cells by RT-qPCR, IHC and Western blot. CCK-8, cloning, Transwell, wound healing assay, flow cytometry and Kits testing detected the effects on cells. EMSA, Yeast one-hybrid, dual-luciferase assays, ChIP-seq analysis and ChIP-PCR analysis were used to verify transcription factor binding, and pull-down assay, CO-IP, and immunofluorescence (IF) were employed to confirm interaction. MYB action was further assessed by subcutaneous tumour experiments in nude mice.

Results: MYB is the key differential expression TF in GC, and SSBP2 is a key target gene of MYB. MYB had a higher expression, but SSBP2 had a lower expression in GC patients' gastric cancer tissue. SSBP2 is a direct target of MYB in GC cells. SSBP2 was transcriptionally repressed by MYB. SSBP2 further negatively regulates ISL1 expression. MYB knockdown inhibited glycolysis, proliferation, invasion, and migration abilities in GC cells both in vivo and in vitro, but it was also reversed by SSBP2 knockdown. SSBP2 overexpression inhibits glycolysis, cell proliferation, invasion and migration abilities in GC cells, but it was reversed by ISL1 overexpression.

Conclusion: MYB mediates glycolysis and malignant progression in GC cells through regulation of the SSBP2/ISL1 axis.

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来源期刊
CiteScore
10.90
自引率
1.70%
发文量
360
审稿时长
1 months
期刊介绍: Cancer Cell International publishes articles on all aspects of cancer cell biology, originating largely from, but not limited to, work using cell culture techniques. The journal focuses on novel cancer studies reporting data from biological experiments performed on cells grown in vitro, in two- or three-dimensional systems, and/or in vivo (animal experiments). These types of experiments have provided crucial data in many fields, from cell proliferation and transformation, to epithelial-mesenchymal interaction, to apoptosis, and host immune response to tumors. Cancer Cell International also considers articles that focus on novel technologies or novel pathways in molecular analysis and on epidemiological studies that may affect patient care, as well as articles reporting translational cancer research studies where in vitro discoveries are bridged to the clinic. As such, the journal is interested in laboratory and animal studies reporting on novel biomarkers of tumor progression and response to therapy and on their applicability to human cancers.
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