Jiaheng Xie, Songyun Zhao, Dan Wu, Yeqi Feng, Chenfeng Ma, Wei Yan, Ming Wang
{"title":"瘢痕疙瘩免疫微环境中的单细胞三角凋亡调控模式。","authors":"Jiaheng Xie, Songyun Zhao, Dan Wu, Yeqi Feng, Chenfeng Ma, Wei Yan, Ming Wang","doi":"10.2147/CCID.S536776","DOIUrl":null,"url":null,"abstract":"<p><strong>Introduction: </strong>Keloids are a complex pathological condition of the skin characterized by abnormal proliferation of fibrous tissue and excessive accumulation of extracellular matrix, typically following inflammation after skin injury. Understanding the regulatory mechanisms of immune cells involved in keloid formation is essential for the development of effective treatments.</p><p><strong>Methods: </strong>This study integrated publicly available single-cell RNA sequencing (scRNA-seq) data with our own keloid scRNA-seq samples to investigate the role of triaptosis in shaping the immune microenvironment of keloids. We analyzed the composition and functional status of fibroblast and immune cell subpopulations.</p><p><strong>Results: </strong>Immune cells in keloids, especially CD8<sup>+</sup> T cells and macrophages, showed significant heterogeneity under the influence of triaptosis regulatory patterns. These triaptosis-associated immune cell clusters exhibited distinct signaling interference compared to mesenchymal fibroblasts and contributed to keloid development. Furthermore, ELMO2 was identified as a key gene with a potential causal relationship to keloids using Summary-data-based Mendelian Randomization and validated through immunofluorescence staining.</p><p><strong>Conclusion: </strong>Our findings reveal the complexity of cell-cell interactions in the keloid immune microenvironment and highlight triaptosis as a potential regulatory mechanism in keloid pathogenesis. The identification of ELMO2 as a key factor offers a promising therapeutic target. This study lays a foundation for developing novel therapeutic strategies and encourages future investigations into the clinical application of triaptosis-related interventions for keloid treatment.</p>","PeriodicalId":10447,"journal":{"name":"Clinical, Cosmetic and Investigational Dermatology","volume":"18 ","pages":"2423-2437"},"PeriodicalIF":2.2000,"publicationDate":"2025-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12476853/pdf/","citationCount":"0","resultStr":"{\"title\":\"The Single-Cell Triaptosis Regulatory Pattern in the Immune Microenvironment of Keloids.\",\"authors\":\"Jiaheng Xie, Songyun Zhao, Dan Wu, Yeqi Feng, Chenfeng Ma, Wei Yan, Ming Wang\",\"doi\":\"10.2147/CCID.S536776\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Introduction: </strong>Keloids are a complex pathological condition of the skin characterized by abnormal proliferation of fibrous tissue and excessive accumulation of extracellular matrix, typically following inflammation after skin injury. Understanding the regulatory mechanisms of immune cells involved in keloid formation is essential for the development of effective treatments.</p><p><strong>Methods: </strong>This study integrated publicly available single-cell RNA sequencing (scRNA-seq) data with our own keloid scRNA-seq samples to investigate the role of triaptosis in shaping the immune microenvironment of keloids. We analyzed the composition and functional status of fibroblast and immune cell subpopulations.</p><p><strong>Results: </strong>Immune cells in keloids, especially CD8<sup>+</sup> T cells and macrophages, showed significant heterogeneity under the influence of triaptosis regulatory patterns. These triaptosis-associated immune cell clusters exhibited distinct signaling interference compared to mesenchymal fibroblasts and contributed to keloid development. Furthermore, ELMO2 was identified as a key gene with a potential causal relationship to keloids using Summary-data-based Mendelian Randomization and validated through immunofluorescence staining.</p><p><strong>Conclusion: </strong>Our findings reveal the complexity of cell-cell interactions in the keloid immune microenvironment and highlight triaptosis as a potential regulatory mechanism in keloid pathogenesis. The identification of ELMO2 as a key factor offers a promising therapeutic target. This study lays a foundation for developing novel therapeutic strategies and encourages future investigations into the clinical application of triaptosis-related interventions for keloid treatment.</p>\",\"PeriodicalId\":10447,\"journal\":{\"name\":\"Clinical, Cosmetic and Investigational Dermatology\",\"volume\":\"18 \",\"pages\":\"2423-2437\"},\"PeriodicalIF\":2.2000,\"publicationDate\":\"2025-09-24\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12476853/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Clinical, Cosmetic and Investigational Dermatology\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.2147/CCID.S536776\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2025/1/1 0:00:00\",\"PubModel\":\"eCollection\",\"JCR\":\"Q3\",\"JCRName\":\"DERMATOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Clinical, Cosmetic and Investigational Dermatology","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.2147/CCID.S536776","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/1/1 0:00:00","PubModel":"eCollection","JCR":"Q3","JCRName":"DERMATOLOGY","Score":null,"Total":0}
The Single-Cell Triaptosis Regulatory Pattern in the Immune Microenvironment of Keloids.
Introduction: Keloids are a complex pathological condition of the skin characterized by abnormal proliferation of fibrous tissue and excessive accumulation of extracellular matrix, typically following inflammation after skin injury. Understanding the regulatory mechanisms of immune cells involved in keloid formation is essential for the development of effective treatments.
Methods: This study integrated publicly available single-cell RNA sequencing (scRNA-seq) data with our own keloid scRNA-seq samples to investigate the role of triaptosis in shaping the immune microenvironment of keloids. We analyzed the composition and functional status of fibroblast and immune cell subpopulations.
Results: Immune cells in keloids, especially CD8+ T cells and macrophages, showed significant heterogeneity under the influence of triaptosis regulatory patterns. These triaptosis-associated immune cell clusters exhibited distinct signaling interference compared to mesenchymal fibroblasts and contributed to keloid development. Furthermore, ELMO2 was identified as a key gene with a potential causal relationship to keloids using Summary-data-based Mendelian Randomization and validated through immunofluorescence staining.
Conclusion: Our findings reveal the complexity of cell-cell interactions in the keloid immune microenvironment and highlight triaptosis as a potential regulatory mechanism in keloid pathogenesis. The identification of ELMO2 as a key factor offers a promising therapeutic target. This study lays a foundation for developing novel therapeutic strategies and encourages future investigations into the clinical application of triaptosis-related interventions for keloid treatment.
期刊介绍:
Clinical, Cosmetic and Investigational Dermatology is an international, peer-reviewed, open access journal that focuses on the latest clinical and experimental research in all aspects of skin disease and cosmetic interventions. Normal and pathological processes in skin development and aging, their modification and treatment, as well as basic research into histology of dermal and dermal structures that provide clinical insights and potential treatment options are key topics for the journal.
Patient satisfaction, preference, quality of life, compliance, persistence and their role in developing new management options to optimize outcomes for target conditions constitute major areas of interest.
The journal is characterized by the rapid reporting of clinical studies, reviews and original research in skin research and skin care.
All areas of dermatology will be covered; contributions will be welcomed from all clinicians and basic science researchers globally.