Michelle Glass, Nahid Khalajiassadi, Shuli Chen, Dharshini Ganeshan, Hannah Partington, David B Finlay
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Development of a Cell Membrane Conformational Cannabinoid CB1 Receptor Biosensor for the Detection of Synthetic Cannabinoids.
This study aimed to develop a chemical structure-independent, sensitive screening platform for detecting novel synthetic cannabinoids using isolated cell membranes. Resonance energy transfer techniques were employed to detect conformational changes in the CB1 receptor upon agonist binding. Eight cannabinoid CB1 conformational biosensors were generated and characterized featuring different tag compositions and placements. The biosensors were initially tested in live cell assays, before conditions were optimized for activity in isolated cell membranes. The optimal sensor developed could successfully distinguish between different agonist efficacy and potency profiles. This biosensor allows for the detection of synthetic cannabinoids without relying on their chemical structure, potentially providing a valuable tool for compliance agencies to rapidly respond to the proliferation of new psychoactive substances. The ability to differentiate efficacy and potency profiles may also contribute to harm reduction strategies upon detection of these compounds.
期刊介绍：
ACS Sensors is a peer-reviewed research journal that focuses on the dissemination of new and original knowledge in the field of sensor science, particularly those that selectively sense chemical or biological species or processes. The journal covers a broad range of topics, including but not limited to biosensors, chemical sensors, gas sensors, intracellular sensors, single molecule sensors, cell chips, and microfluidic devices. It aims to publish articles that address conceptual advances in sensing technology applicable to various types of analytes or application papers that report on the use of existing sensing concepts in new ways or for new analytes.
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