解码肠道共生信号:秋葵脂多糖的结构和免疫学分析。

IF 16.9 1区 化学 Q1 CHEMISTRY, MULTIDISCIPLINARY
Luca De Simone Carone,Giusi Barra,Roberta Cirella,Marcello Ziaco,Marcello Mercogliano,Francesca Olmeo,Emanuela Andretta,Valentina Mazziotti,Carmela Fusco,Giuliana D'Ippolito,Katarzyna Anna Duda,Freda M Farquharson,Petra Louis,Angelo Fontana,Alba Silipo,Antonio Molinaro,Fabrizio Chiodo,Flaviana Di Lorenzo
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引用次数: 0

摘要

来自肠道微生物群的脂多糖(LPS)的免疫作用仍然很少被探索,被长期以来认为LPS是一种典型的促炎分子的观点所掩盖。本文首次报道了人类口腔和肠道微生物群中重要的一员——secgatella copri DSM 18205的LPS的化学和免疫学特性。该脂多糖具有独特的化学结构,包括富含甘露糖和葡萄糖的寡糖(OS)和高度不均匀的低酰化脂质a结构域,经先进的质谱(MS)和核磁共振(NMR)谱分析证实。在功能上,葡萄多糖表现出TLR4激活减弱和弱促炎活性。引人注目的是,通过飞行时间(CyTOF)的高维细胞术显示CD14+CD16+单核细胞的选择性保存,这些免疫亚群通常被典型肠杆菌lps耗尽。这些发现确定了葡萄球菌脂多糖是一种化学和功能上独特的微生物特征,为宿主-微生物群免疫串扰提供了新的见解,并突出了其在微生物群免疫调节策略方面的潜力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Decoding a Gut Commensal Signal: Structural and Immunological Profiling of Segatella Copri Lipopolysaccharide.
The immunological effects of lipopolysaccharides (LPSs) from gut microbiota remain poorly explored, overshadowed by the longstanding view of LPS as a prototypical pro-inflammatory molecule. Herein, we report the first comprehensive chemical and immunological characterization of LPS from Segatella copri DSM 18205, a prominent member of the human oral and intestinal microbiota. This LPS features a unique chemical architecture, including a mannose- and glucose-rich oligosaccharide (OS) and a highly heterogeneous, hypo-acylated lipid A domain, as elucidated by advanced mass spectrometry (MS) and nuclear magnetic resonance (NMR) spectroscopy. Functionally, S. copri LPS displayed attenuated TLR4 activation and weak pro-inflammatory activity. Strikingly, high-dimensional cytometry by time-of-flight (CyTOF) revealed a selective preservation of CD14+CD16+ monocytes, immune subsets typically depleted by canonical enterobacterial LPSs. These findings identify S. copri LPS as a chemically and functionally distinct microbial signature, offering new insights into host-microbiota immune crosstalk and highlighting its potential for microbiome-informed immunomodulatory strategies.
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来源期刊
CiteScore
26.60
自引率
6.60%
发文量
3549
审稿时长
1.5 months
期刊介绍: Angewandte Chemie, a journal of the German Chemical Society (GDCh), maintains a leading position among scholarly journals in general chemistry with an impressive Impact Factor of 16.6 (2022 Journal Citation Reports, Clarivate, 2023). Published weekly in a reader-friendly format, it features new articles almost every day. Established in 1887, Angewandte Chemie is a prominent chemistry journal, offering a dynamic blend of Review-type articles, Highlights, Communications, and Research Articles on a weekly basis, making it unique in the field.
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