{"title":"光合植物来源的纳米囊泡通过光活性氧生成精确放大光动力效应,增强癌症光免疫治疗。","authors":"Jiangang Mei,Weilun Pan,Bo Li,Mingzhen Zhong,Xiudong Shi,Yanfang Cheng,Bodeng Wu,Qi Xiu,Yicong Xue,Bo Situ,Lei Zheng","doi":"10.1021/acsnano.5c13350","DOIUrl":null,"url":null,"abstract":"Photodynamic immunotherapy has emerged as a promising cancer therapeutic strategy, yet its efficacy is crucially hindered by the hypoxic and immunosuppressive tumor microenvironment (TME). Herein, we present a bioinspired nanoplatform that leverages the natural photosynthetic capabilities of spinach-derived nanovesicles (SDNV) for light-excited oxygen evolution to address this critical challenge. SDNV is engineered to encapsulate aggregation-induced emission luminogens (AIEgen), forming AIE@SDNV nanoparticles with excellent biocompatibility and transmembrane permeability. Upon irradiation, SDNV generates substantial oxygen as a substance for AIEgen to produce reactive oxygen species, thus improving the photodynamic efficacy by triggering severe cellular lipid peroxidation and calcium ion imbalance. This leads to potent tumor cell destruction and immunogenic cell death. Subsequently, significant release of damage-associated molecular patterns from tumor cells enhances systemic antitumor immunity via the cGAS-STING signaling pathway and activates immune responses within the TME. Moreover, SDNV enables precise AIEgen delivery and prolonged tumor retention. Simultaneously, AIE@SDNV-mediated photoimmunotherapy effectively suppresses both primary and distant tumors in a bilateral tumor model. This study provides a promising strategy for efficiently delivering a therapeutic agent, improving hypoxia-restricted photodynamic therapy, and reversing the immunosuppressive TME, thereby achieving potentiated antitumor efficacy and highlighting the potential of plant-derived nanovesicles in advancing cancer nanomedicine.","PeriodicalId":21,"journal":{"name":"ACS Nano","volume":"3 1","pages":""},"PeriodicalIF":16.0000,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Photosynthetic Plant-Derived Nanovesicles Precisely Amplify Photodynamic Effect by Light-Activated Oxygen Generation for Enhanced Cancer Photoimmunotherapy.\",\"authors\":\"Jiangang Mei,Weilun Pan,Bo Li,Mingzhen Zhong,Xiudong Shi,Yanfang Cheng,Bodeng Wu,Qi Xiu,Yicong Xue,Bo Situ,Lei Zheng\",\"doi\":\"10.1021/acsnano.5c13350\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Photodynamic immunotherapy has emerged as a promising cancer therapeutic strategy, yet its efficacy is crucially hindered by the hypoxic and immunosuppressive tumor microenvironment (TME). Herein, we present a bioinspired nanoplatform that leverages the natural photosynthetic capabilities of spinach-derived nanovesicles (SDNV) for light-excited oxygen evolution to address this critical challenge. SDNV is engineered to encapsulate aggregation-induced emission luminogens (AIEgen), forming AIE@SDNV nanoparticles with excellent biocompatibility and transmembrane permeability. Upon irradiation, SDNV generates substantial oxygen as a substance for AIEgen to produce reactive oxygen species, thus improving the photodynamic efficacy by triggering severe cellular lipid peroxidation and calcium ion imbalance. This leads to potent tumor cell destruction and immunogenic cell death. Subsequently, significant release of damage-associated molecular patterns from tumor cells enhances systemic antitumor immunity via the cGAS-STING signaling pathway and activates immune responses within the TME. Moreover, SDNV enables precise AIEgen delivery and prolonged tumor retention. Simultaneously, AIE@SDNV-mediated photoimmunotherapy effectively suppresses both primary and distant tumors in a bilateral tumor model. This study provides a promising strategy for efficiently delivering a therapeutic agent, improving hypoxia-restricted photodynamic therapy, and reversing the immunosuppressive TME, thereby achieving potentiated antitumor efficacy and highlighting the potential of plant-derived nanovesicles in advancing cancer nanomedicine.\",\"PeriodicalId\":21,\"journal\":{\"name\":\"ACS Nano\",\"volume\":\"3 1\",\"pages\":\"\"},\"PeriodicalIF\":16.0000,\"publicationDate\":\"2025-09-30\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"ACS Nano\",\"FirstCategoryId\":\"88\",\"ListUrlMain\":\"https://doi.org/10.1021/acsnano.5c13350\",\"RegionNum\":1,\"RegionCategory\":\"材料科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"CHEMISTRY, MULTIDISCIPLINARY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"ACS Nano","FirstCategoryId":"88","ListUrlMain":"https://doi.org/10.1021/acsnano.5c13350","RegionNum":1,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, MULTIDISCIPLINARY","Score":null,"Total":0}
Photosynthetic Plant-Derived Nanovesicles Precisely Amplify Photodynamic Effect by Light-Activated Oxygen Generation for Enhanced Cancer Photoimmunotherapy.
Photodynamic immunotherapy has emerged as a promising cancer therapeutic strategy, yet its efficacy is crucially hindered by the hypoxic and immunosuppressive tumor microenvironment (TME). Herein, we present a bioinspired nanoplatform that leverages the natural photosynthetic capabilities of spinach-derived nanovesicles (SDNV) for light-excited oxygen evolution to address this critical challenge. SDNV is engineered to encapsulate aggregation-induced emission luminogens (AIEgen), forming AIE@SDNV nanoparticles with excellent biocompatibility and transmembrane permeability. Upon irradiation, SDNV generates substantial oxygen as a substance for AIEgen to produce reactive oxygen species, thus improving the photodynamic efficacy by triggering severe cellular lipid peroxidation and calcium ion imbalance. This leads to potent tumor cell destruction and immunogenic cell death. Subsequently, significant release of damage-associated molecular patterns from tumor cells enhances systemic antitumor immunity via the cGAS-STING signaling pathway and activates immune responses within the TME. Moreover, SDNV enables precise AIEgen delivery and prolonged tumor retention. Simultaneously, AIE@SDNV-mediated photoimmunotherapy effectively suppresses both primary and distant tumors in a bilateral tumor model. This study provides a promising strategy for efficiently delivering a therapeutic agent, improving hypoxia-restricted photodynamic therapy, and reversing the immunosuppressive TME, thereby achieving potentiated antitumor efficacy and highlighting the potential of plant-derived nanovesicles in advancing cancer nanomedicine.
期刊介绍:
ACS Nano, published monthly, serves as an international forum for comprehensive articles on nanoscience and nanotechnology research at the intersections of chemistry, biology, materials science, physics, and engineering. The journal fosters communication among scientists in these communities, facilitating collaboration, new research opportunities, and advancements through discoveries. ACS Nano covers synthesis, assembly, characterization, theory, and simulation of nanostructures, nanobiotechnology, nanofabrication, methods and tools for nanoscience and nanotechnology, and self- and directed-assembly. Alongside original research articles, it offers thorough reviews, perspectives on cutting-edge research, and discussions envisioning the future of nanoscience and nanotechnology.