通过转录组数据挖掘鉴定与内生尖孢镰刀菌相关的新型γ - artitivirus。

Q2 Medicine

VirusDisease Pub Date : 2025-06-01 Epub Date: 2025-04-02 DOI:10.1007/s13337-025-00918-w

Megha Das, A I Bhat

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引用次数: 0

摘要

下一代测序（NGS）和生物信息学分析使得从公共领域的转录组数据集鉴定病毒更加流行。在这里，我们已经鉴定了一种新的dsRNA分枝病毒属的编码完整的基因组序列，伽玛病毒来自内生镰刀菌（Fusarium oxysporum），其名称为镰刀菌（Fusarium oxysporum partitivirus 2）。它有一个分裂的单顺反子基因组，有1742 bp长的dsRNA 1和1421 bp长的dsRNA 2。dsRNA1和dsRNA2含有一个开放阅读框（ORF），分别编码536个氨基酸的RNA依赖RNA聚合酶（RdRp）和419个氨基酸的外壳蛋白。本文首次报道了利用印度尖孢镰刀菌转录组数据挖掘鉴定出的真菌病毒。补充信息：在线版本包含补充资料，提供地址：10.1007/s13337-025-00918-w。

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Identification of a novel Gammapartitivirus associated with endophytic Fusarium oxysporum through transcriptome dataset mining.

Next Generation Sequencing (NGS) and bioinformatic analysis have made identifying viruses from transcriptome datasets in the public domain more popular. Here, we have identified the coding-complete genome sequence of a novel dsRNA mycovirus of the genus, Gammapartitivirus from an endophytic Fusarium oxysporum for which the name, Fusarium oxysporum partitivirus 2 is proposed. It has a bisgmented, monocistronic genome with 1742 bp long dsRNA 1 and 1421 bp long dsRNA 2. The dsRNA1 and dsRNA2 contain a single open reading frame (ORF) coding for the RNA-dependent RNA polymerase (RdRp) of 536 amino acids and the coat protein of 419 amino acids, respectively. This is the first report of a fungal virus identified by the transcriptome data mining of Fusarium oxysporum from India.

Supplementary information: The online version contains supplementary material available at 10.1007/s13337-025-00918-w.

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来源期刊

VirusDisease Medicine-Infectious Diseases

CiteScore

7.00

自引率

0.00%

发文量

期刊介绍： VirusDisease, formerly known as ''Indian Journal of Virology'', publishes original research on all aspects of viruses infecting animal, human, plant, fish and other living organisms.