psathyrellacae科真菌是异养型丹晓兰(Danxiaorchis yangii)的共生伙伴。

IF 3.8 2区 生物学 Q2 MYCOLOGY
Chunyan Leng, Guo Yang, Mengyan Hou, Xinjie Huang, Yongmei Xing, Boyun Yang, Juan Chen
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引用次数: 0

摘要

完全真菌异养兰花完全依赖真菌共生体来获取碳,并且通常高度专业化于它们的真菌关联。杨丹小兰花是一种完全分枝异养的兰科植物,在中国东南部的特有种区种群数量极为有限。它的真菌共生体仍然知之甚少。在这项研究中,我们通过分离可培养的真菌内生菌和对核糖体内转录间隔-1 (ITS1)区域进行高通量测序来研究杨氏霉的真菌关联。从杨花根状茎(地下茎)中分离到6株Psathyrellaceae,系统发育分析表明它们属于两个主要分类群。高通量测序进一步证实根状茎内真菌群落以Psathyrellaceae为主。此外,体外共生种子萌发试验表明,其中一株分离菌株能促进萌发种子生长至原球茎期。这些发现对于提高我们对杨氏分枝杆菌异养共生的认识具有重要意义。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Fungi of the family Psathyrellaceae are symbiotic partners of the mycoheterotrophic orchid Danxiaorchis yangii.

Fully mycoheterotrophic orchids rely entirely on fungal symbionts for carbon acquisition and are often highly specialized in their fungal associations. Danxiaorchis yangii is a fully mycoheterotrophic orchid species with an extremely limited population in its endemic region of southeastern China. Its fungal symbionts remain poorly understood. In this study, we investigated the fungal associations of D. yangii using both the isolation of culturable fungal endophytes and high-throughput sequencing of the ribosomal internal transcribed spacer-1 (ITS1) region. Six strains of Psathyrellaceae were isolated from rhizomes (underground stems) of D. yangii and phylogenetic analysis revealed that they belong to two main taxa. High-throughput sequencing further confirmed that the fungal community within the rhizomes was dominated by Psathyrellaceae. Moreover, an in vitro symbiotic seed germination assay demonstrated that one of the isolated strains could promote the growth of germinating seeds to the protocorm stage. These findings are significant for advancing our understanding of the mycoheterotrophic symbiosis in D. yangii.

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来源期刊
Mycorrhiza
Mycorrhiza 生物-真菌学
CiteScore
8.20
自引率
2.60%
发文量
40
审稿时长
6-12 weeks
期刊介绍: Mycorrhiza is an international journal devoted to research into mycorrhizas - the widest symbioses in nature, involving plants and a range of soil fungi world-wide. The scope of Mycorrhiza covers all aspects of research into mycorrhizas, including molecular biology of the plants and fungi, fungal systematics, development and structure of mycorrhizas, and effects on plant physiology, productivity, reproduction and disease resistance. The scope also includes interactions between mycorrhizal fungi and other soil organisms and effects of mycorrhizas on plant biodiversity and ecosystem structure. Mycorrhiza contains original papers, short notes and review articles, along with commentaries and news items. It forms a platform for new concepts and discussions, and is a basis for a truly international forum of mycorrhizologists from all over the world.
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