木麻黄小泡包膜发育缺陷突变体的研究。

IF 2 4区环境科学与生态学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Microbes and Environments Pub Date : 2025-01-01 DOI:10.1264/jsme2.ME25037

Ken-Ichi Kucho, Kosuke Taniyama

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引用次数: 0

摘要

法兰克菌是一种固氮放线菌，它形成了一种独特的多细胞结构，称为囊泡，专门用于固氮。囊泡被一层厚的类hopanoid脂质包膜包围，作为氧气渗透的屏障，防止氮酶失活。五个突变体产生的囊泡数量与野生型相似；然而，他们没能固定N2。5个突变体的囊泡膜厚度均减小，囊泡内氧浓度升高。因此，这些突变体由于氧气渗透到囊泡中引起的氮酶失活而无法固定N2。

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Characterization of Frankia casuarinae Mutants Defective in Vesicle Envelope Development.

Frankia, a nitrogen-fixing actinobacterium, forms a unique multicellular structure known as a vesicle that is dedicated to nitrogen fixation. The vesicle is surrounded by a thick hopanoid lipid envelope that acts as a barrier against oxygen penetration, preventing nitrogenase inactivation. Five mutants produced a similar number of vesicles to the wild type; however, they failed to fix N₂. The thickness of vesicle envelopes was reduced in all five mutants, and the oxygen concentration increased inside the vesicles of four mutants. Therefore, these mutants were unable to fix N₂ due to the inactivation of nitrogenase caused by oxygen penetration into the vesicles.

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来源期刊

Microbes and Environments 生物-生物工程与应用微生物

CiteScore

4.10

自引率

13.60%

发文量

审稿时长

3 months

期刊介绍： Microbial ecology in natural and engineered environments; Microbial degradation of xenobiotic compounds; Microbial processes in biogeochemical cycles; Microbial interactions and signaling with animals and plants; Interactions among microorganisms; Microorganisms related to public health; Phylogenetic and functional diversity of microbial communities; Genomics, metagenomics, and bioinformatics for microbiology; Application of microorganisms to agriculture, fishery, and industry; Molecular biology and biochemistry related to environmental microbiology; Methodology in general and environmental microbiology; Interdisciplinary research areas for microbial ecology (e.g., Astrobiology, and Origins of Life); Taxonomic description of novel microorganisms with ecological perspective; Physiology and metabolisms of microorganisms; Evolution of genes and microorganisms; Genome report of microorganisms with ecological perspective.