{"title":"连续蓝光与脉冲蓝光对粪肠球菌生物膜还原效果的比较。","authors":"Emad Kosarieh, Sahar Sattari Khavas, Nasim Chiniforush, Shima Afrasiabi","doi":"10.1007/s10103-025-04663-w","DOIUrl":null,"url":null,"abstract":"<p><p>Mature biofilms exhibited minimal antimicrobial susceptibility. This study compares the decontamination effect of 450 nm continuous wave (CW) and pulsed wave (PW) blue diode laser with two different photosensitizers (curcumin and riboflavin) against mature Enterococcus faecalis biofilm model. Four irradiation parameter sets A (CW, peak power 0.4 W, and average fluence of 24 J/cm<sup>2</sup>), B (PW, duty cycle (DC) 50%, peak power 0.8 W, and average fluence of 24 J/cm<sup>2</sup>), C (CW, peak power 0.5 W, and average fluence of 30 J/cm<sup>2</sup>), and D (PW, DC 50%, peak power 1.0 W, and average fluence of 30 J/cm<sup>2</sup>) were used. The results showed that all irradiation parameters achieved a significant bacterial reduction compared to the control (p < 0.001). However, PW showed a more pronounced reduction effect than CW, with the difference being particularly marked at an average fluence of 30 J/cm<sup>2</sup>. Curcumin or riboflavin without 450 nm blue diode laser irradiation had no antimicrobial effect (p > 0.05). PW light has the potential to improve the efficacy of a blue diode laser. However, the correct choice of parameters is required to achieve adequate laser fluence.</p>","PeriodicalId":17978,"journal":{"name":"Lasers in Medical Science","volume":"40 1","pages":"396"},"PeriodicalIF":2.4000,"publicationDate":"2025-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Comparison of the effects of continuous and pulsed blue light for reduction of Enterococcus faecalis biofilms.\",\"authors\":\"Emad Kosarieh, Sahar Sattari Khavas, Nasim Chiniforush, Shima Afrasiabi\",\"doi\":\"10.1007/s10103-025-04663-w\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Mature biofilms exhibited minimal antimicrobial susceptibility. This study compares the decontamination effect of 450 nm continuous wave (CW) and pulsed wave (PW) blue diode laser with two different photosensitizers (curcumin and riboflavin) against mature Enterococcus faecalis biofilm model. Four irradiation parameter sets A (CW, peak power 0.4 W, and average fluence of 24 J/cm<sup>2</sup>), B (PW, duty cycle (DC) 50%, peak power 0.8 W, and average fluence of 24 J/cm<sup>2</sup>), C (CW, peak power 0.5 W, and average fluence of 30 J/cm<sup>2</sup>), and D (PW, DC 50%, peak power 1.0 W, and average fluence of 30 J/cm<sup>2</sup>) were used. The results showed that all irradiation parameters achieved a significant bacterial reduction compared to the control (p < 0.001). However, PW showed a more pronounced reduction effect than CW, with the difference being particularly marked at an average fluence of 30 J/cm<sup>2</sup>. Curcumin or riboflavin without 450 nm blue diode laser irradiation had no antimicrobial effect (p > 0.05). PW light has the potential to improve the efficacy of a blue diode laser. However, the correct choice of parameters is required to achieve adequate laser fluence.</p>\",\"PeriodicalId\":17978,\"journal\":{\"name\":\"Lasers in Medical Science\",\"volume\":\"40 1\",\"pages\":\"396\"},\"PeriodicalIF\":2.4000,\"publicationDate\":\"2025-09-29\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Lasers in Medical Science\",\"FirstCategoryId\":\"5\",\"ListUrlMain\":\"https://doi.org/10.1007/s10103-025-04663-w\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"ENGINEERING, BIOMEDICAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Lasers in Medical Science","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.1007/s10103-025-04663-w","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"ENGINEERING, BIOMEDICAL","Score":null,"Total":0}
Comparison of the effects of continuous and pulsed blue light for reduction of Enterococcus faecalis biofilms.
Mature biofilms exhibited minimal antimicrobial susceptibility. This study compares the decontamination effect of 450 nm continuous wave (CW) and pulsed wave (PW) blue diode laser with two different photosensitizers (curcumin and riboflavin) against mature Enterococcus faecalis biofilm model. Four irradiation parameter sets A (CW, peak power 0.4 W, and average fluence of 24 J/cm2), B (PW, duty cycle (DC) 50%, peak power 0.8 W, and average fluence of 24 J/cm2), C (CW, peak power 0.5 W, and average fluence of 30 J/cm2), and D (PW, DC 50%, peak power 1.0 W, and average fluence of 30 J/cm2) were used. The results showed that all irradiation parameters achieved a significant bacterial reduction compared to the control (p < 0.001). However, PW showed a more pronounced reduction effect than CW, with the difference being particularly marked at an average fluence of 30 J/cm2. Curcumin or riboflavin without 450 nm blue diode laser irradiation had no antimicrobial effect (p > 0.05). PW light has the potential to improve the efficacy of a blue diode laser. However, the correct choice of parameters is required to achieve adequate laser fluence.
期刊介绍：
Lasers in Medical Science (LIMS) has established itself as the leading international journal in the rapidly expanding field of medical and dental applications of lasers and light. It provides a forum for the publication of papers on the technical, experimental, and clinical aspects of the use of medical lasers, including lasers in surgery, endoscopy, angioplasty, hyperthermia of tumors, and photodynamic therapy. In addition to medical laser applications, LIMS presents high-quality manuscripts on a wide range of dental topics, including aesthetic dentistry, endodontics, orthodontics, and prosthodontics.
The journal publishes articles on the medical and dental applications of novel laser technologies, light delivery systems, sensors to monitor laser effects, basic laser-tissue interactions, and the modeling of laser-tissue interactions. Beyond laser applications, LIMS features articles relating to the use of non-laser light-tissue interactions.
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