Efficient bioconversion of corn stover hydrolysates into phenazine-1-carboxylic acid by engineered Pseudomonas chlororaphis for sustainable biopesticide production

The conversion of lignocellulose into value-added chemicals provides a promising solution for replacing petrochemical resources, reducing environmental impact, and promoting the transition to green production. The hydrolysis products of lignocellulosic biomass are abundant in glucose, xylose, and arabinose, and the efficient conversion of these sugars through various biotechnological processes is crucial for maximizing the economic and environmental benefits of lignocellulosic biomass as a renewable resource. In this work, we engineered a strain of Pseudomonas chlororaphis that efficiently produces phenazine-1-carboxylic acid (PCA), a biopesticide registered in China as ’Shenqinmycin’, from corn stover hydrolysates. The genome-scale metabolic model of P. putida (iJN1462) was adapted for P. chlororaphis GP72 by incorporating two additional pathways. Using this adapted model, we engineered the strain P. chloriraphis GP72, which completely consumed all the sugars in corn stover hydrolysate and converted them into PCA, with a yield of 384.2 mg/L. This is the first report to display the full utilization of sugars in corn stover hydrolysate for PCA production. This work demonstrates the potential of P. chlororaphis in utilizing corn stover hydrolysate for PCA biosynthesis and provides valuable references for the biosynthesis of other value-added chemicals in lignocellulosic biomass using Pseudomonas.