ph响应纳米级混合配体金属有机骨架作为光敏剂载体在靶向抗菌光动力治疗中的应用。

IF 3.4 4区 医学 Q2 CHEMISTRY, MEDICINAL
ChemMedChem Pub Date : 2025-09-28 DOI:10.1002/cmdc.202500510
Fathima Fasna P H, Sreesha Sasi, Hareesh N Ramanathan, Jasna Vijayan, Mohamed Hatha Abdulla Ammanamveetil
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引用次数: 0

摘要

不断升级的多重耐药细菌感染强调了寻找常规抗生素替代品的迫切需要。抗菌光动力疗法是一种很有前途的治疗策略,利用亚甲基蓝(MB)等光活化光敏剂产生杀菌活性氧(ROS),破坏微生物膜和DNA。这种方法最大限度地减少了由于活性氧的非特异性作用而产生的耐药性,并证明了对革兰氏阳性和革兰氏阴性病原体的有效性。合成了一种pH响应型混合配体金属-有机骨架(ML-MOF)作为MB的纳米载体。将MB包封在ML-MOF (MB@ML-MOF50)中,负载容量为29.67%,在pH 5.1(模拟感染条件)下控释率为85%。MB@ML-MOF50的单线态产氧效率(S = 0.2098)是游离MB (S = 0.1058)的两倍,证实了其光动力活性增强。MB@ML-MOF50在650 nm激光照射下,在25µM下实现了完全的细菌抑制(0%存活率),超过了游离MB。使用结晶紫(CV)法进行的生物膜根除研究显示,与未照射对照相比,激光照射15分钟后,大肠杆菌(E. coli)生物膜的抑制率为37.26%,金黄色葡萄球菌(S. aureus)生物膜的抑制率为25.42%,这表明激光照射可能会破坏持续感染。MB@ML-MOF50是一个很有前途的多功能纳米平台,用于靶向,ph响应和增强光动力抗菌治疗。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
pH-Responsive Nanoscale Mixed Ligand Metal Organic Framework as a Carrier for Photosensitizer in Targeted Antibacterial Photodynamic Therapy.

The escalating multidrug-resistant bacterial infections underscore the urgent need for alternatives to conventional antibiotics. Antimicrobial photodynamic therapy emerges as a promising strategy, leveraging light-activated photosensitizers like methylene blue (MB) to generate bactericidal reactive oxygen species (ROS) that disrupt microbial membranes and DNA. This approach minimizes resistance development due to the nonspecific action of ROS and demonstrates efficacy against both Gram-positive and Gram-negative pathogens. A pH-responsive mixed-ligand metal-organic framework (ML-MOF) is synthesized as a nanocarrier for MB. Encapsulation of MB into ML-MOF (MB@ML-MOF50) resulted in a loading capacity of 29.67%, with a controlled and sustained release profile of 85% at pH 5.1 (infection-mimicking conditions). MB@ML-MOF50 exhibited twice the singlet oxygen generation efficiency (S = 0.2098) compared to free MB (S = 0.1058), confirming enhanced photodynamic activity. MB@ML-MOF50 under 650 nm laser irradiation achieved complete bacterial inhibition (0% survival) at 25 µM, surpassing free MB. Biofilm eradication studies using the crystal violet (CV) assay revealed 37.26% inhibition of Escherichia coli (E. coli) biofilm and 25.42% inhibition of Staphylococcus aureus (S. aureus) biofilm after 15 min of laser exposure compared to a nonirradiated control, indicating the potential for disrupting persistent infections. MB@ML-MOF50 is a promising multifunctional nanoplatform for targeted, pH-responsive, and enhanced photodynamic antibacterial therapy.

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来源期刊
ChemMedChem
ChemMedChem 医学-药学
CiteScore
6.70
自引率
2.90%
发文量
280
审稿时长
1 months
期刊介绍: Quality research. Outstanding publications. With an impact factor of 3.124 (2019), ChemMedChem is a top journal for research at the interface of chemistry, biology and medicine. It is published on behalf of Chemistry Europe, an association of 16 European chemical societies. ChemMedChem publishes primary as well as critical secondary and tertiary information from authors across and for the world. Its mission is to integrate the wide and flourishing field of medicinal and pharmaceutical sciences, ranging from drug design and discovery to drug development and delivery, from molecular modeling to combinatorial chemistry, from target validation to lead generation and ADMET studies. ChemMedChem typically covers topics on small molecules, therapeutic macromolecules, peptides, peptidomimetics, and aptamers, protein-drug conjugates, nucleic acid therapies, and beginning 2017, nanomedicine, particularly 1) targeted nanodelivery, 2) theranostic nanoparticles, and 3) nanodrugs. Contents ChemMedChem publishes an attractive mixture of: Full Papers and Communications Reviews and Minireviews Patent Reviews Highlights and Concepts Book and Multimedia Reviews.
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