多铜氧化酶在金电极直接电子转移下产生H2O2的操作评价。

IF 4.2 2区化学 Q2 CHEMISTRY, MULTIDISCIPLINARY

Chemical Communications Pub Date : 2025-09-29 DOI:10.1039/d5cc03482e

Panpan Wang, Vânia Brissos, Lígia O Martins, Wolfgang Schuhmann, Felipe Conzuelo

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引用次数: 0

摘要

更深入地了解生物/非生物界面复杂的氧化还原过程需要先进的分析工具。我们使用专用的微生物传感器对吸附在金电极上的多铜氧化酶进行了定位和实时表征，提供了详细的和空间分辨的信息。酶促O2还原显示在没有检测到H2O2积累的情况下进行。相反，H2O2来源于金表面的直接O2转化。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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In operando assessment of H₂O₂ production by a multicopper oxidase under direct electron transfer with a gold electrode.

A deeper understanding of the intricate redox processes at biotic/abiotic interfaces requires advanced analytical tools. We present the localized and real-time characterization of a multicopper oxidase adsorbed on a gold electrode using a dedicated microbiosensor, providing detailed and spatially resolved information. Enzymatic O₂ reduction is shown to proceed without detectable H₂O₂ accumulation. Instead, H₂O₂ originates from direct O₂ conversion at the gold surface.

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来源期刊

Chemical Communications 化学-化学综合

CiteScore

8.60

自引率

4.10%

发文量

2705

审稿时长

1.4 months

期刊介绍： ChemComm (Chemical Communications) is renowned as the fastest publisher of articles providing information on new avenues of research, drawn from all the world''s major areas of chemical research.