纳米颗粒穿过粘膜屏障:用单颗粒跟踪区分粘膜粘附和粘膜穿透

IF 11.5 1区 医学 Q1 CHEMISTRY, MULTIDISCIPLINARY
Matteo Tollemeto , Lasse H.E. Thamdrup , Nikos S. Hatzakis , Claus-Michael Lehr , Jan van Hest , Anja Boisen
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引用次数: 0

摘要

粘液是一种粘弹性、选择性渗透的屏障,可保护全身上皮表面。其复杂的结构对基于纳米颗粒的药物递送提出了重大挑战,需要在黏液粘附(延长停留时间)和黏液穿透(进入底层组织)之间取得平衡。这些过程因解剖部位的不同而不同,这是由于粘液周转和成分的不同。目前的方法往往不能明确区分黏液粘连和黏液穿透。在这里,我们强调单粒子跟踪(SPT)是解决这一差距的有力方法。通过捕捉黏液中单个纳米颗粒的轨迹,SPT提供了对其扩散行为和相互作用动力学的高分辨率洞察。值得注意的是,在离体黏液中观察到的颗粒行为与体内结果显示出良好的相关性,这使得SPT成为一种很有前途的转化研究工具。尽管有其优势,但由于缺乏标准化的方案和基准材料,SPT在粘膜给药中仍未得到充分利用。我们提倡开发一种具有明确定义的表面特性(如电荷、疏水性)的纳米颗粒测试集,并常规报告扩散系数和均方位移等关键参数。这些步骤对于提高可重复性、支持交叉研究比较和加速粘膜纳米医学的进展至关重要。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Nanoparticles across mucosal barriers: Differentiating mucoadhesion from mucopenetration using single particle tracking

Nanoparticles across mucosal barriers: Differentiating mucoadhesion from mucopenetration using single particle tracking

Nanoparticles across mucosal barriers: Differentiating mucoadhesion from mucopenetration using single particle tracking
Mucus is a viscoelastic, selectively permeable barrier that protects epithelial surfaces throughout the body. Its complex structure presents a major challenge for nanoparticle-based drug delivery, requiring a balance between mucoadhesion, to prolong residence time, and mucopenetration, to access underlying tissues. These processes vary by anatomical site due to differences in mucus turnover and composition.
Current methods often fail to clearly distinguish between mucoadhesion and mucopenetration. Here, we highlight single-particle tracking (SPT) as a powerful approach to address this gap. By capturing the trajectories of individual nanoparticles in mucus, SPT offers high-resolution insight into their diffusion behavior and interaction dynamics. Notably, particle behavior observed in ex vivo mucus has shown good correlation with in vivo outcomes, making SPT a promising tool for translational research.
Despite its advantages, SPT remains underutilized in mucosal drug delivery, largely due to a lack of standardized protocols and benchmark materials. We advocate for the development of a nanoparticle test set with well-defined surface properties (e.g., charge, hydrophobicity) and the routine reporting of key parameters such as diffusion coefficients and mean squared displacement. These steps are essential to improve reproducibility, support cross-study comparisons, and accelerate progress in mucosal nanomedicine.
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来源期刊
Journal of Controlled Release
Journal of Controlled Release 医学-化学综合
CiteScore
18.50
自引率
5.60%
发文量
700
审稿时长
39 days
期刊介绍: The Journal of Controlled Release (JCR) proudly serves as the Official Journal of the Controlled Release Society and the Japan Society of Drug Delivery System. Dedicated to the broad field of delivery science and technology, JCR publishes high-quality research articles covering drug delivery systems and all facets of formulations. This includes the physicochemical and biological properties of drugs, design and characterization of dosage forms, release mechanisms, in vivo testing, and formulation research and development across pharmaceutical, diagnostic, agricultural, environmental, cosmetic, and food industries. Priority is given to manuscripts that contribute to the fundamental understanding of principles or demonstrate the advantages of novel technologies in terms of safety and efficacy over current clinical standards. JCR strives to be a leading platform for advancements in delivery science and technology.
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