用结构照明和结构检测定位单分子。

IF 23.4 Q1 OPTICS

Light-Science & Applications Pub Date : 2025-09-29 DOI:10.1038/s41377-025-01980-1

Luciano A Masullo

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引用次数: 0

摘要

一种新的高精度单分子定位方案ISM- flux是利用图像扫描显微镜（ISM）和单光子雪崩二极管（SPAD）阵列探测器实现的MINFLUX。ISM-FLUX的定位范围更大，增强了定位方案的鲁棒性，并且还可能使实验能够独立探测单个荧光团的吸收和发射。

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Localization of single molecules with structured illumination and structured detection.

A new high-precision single-molecule localization scheme, ISM-FLUX, is an implementation of MINFLUX using image-scanning microscopy (ISM) with a single-photon avalanche diode (SPAD) array detector. ISM-FLUX results in a larger localization range, enhancing the robustness of the localization scheme and it also potentially enables experiments in which absorption and emission of a single fluorophore can be probed independently.

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来源期刊

Light-Science & Applications 数理科学, 物理学I, 光学, 凝聚态物性 II ：电子结构、电学、磁学和光学性质, 无机非金属材料, 无机非金属类光电信息与功能材料, 工程与材料, 信息科学, 光学和光电子学, 光学和光电子材料, 非线性光学与量子光学

自引率

0.00%

发文量

803

审稿时长

2.1 months