利鲁唑对七氟醚诱导的成年小鼠喘息的影响。

IF 3.3 3区 医学 Q1 PHYSIOLOGY
Saki Taiji, Takashi Nishino, Hisayo Jin, Mayumi Hashida, Shiroh Isono
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引用次数: 0

摘要

在高氧条件下吸入高浓度七氟醚会引起喘息,类似于小鼠在低氧诱导的喘息中观察到的呼吸模式。这一观察结果,再加上对pre-Bötzinger复体中依赖持续钠电流的爆裂神经元在产生缺氧诱导的喘息中起着至关重要的作用的理解,使我们研究了七氟醚诱导的喘息是否可以通过激活脑干内的这种电流来触发。为此,我们评估了腹腔注射利鲁唑(一种持久性钠通道阻滞剂)对成年小鼠七氟醚诱导的喘息的剂量依赖性作用。10只气管插管,自主呼吸,七氟醚麻醉的小鼠。7只小鼠随机接受三种剂量的利鲁唑腹腔注射(6、12、18 mg/kg, i.p),间隔约四周。七氟醚吸入浓度从0.8 MAC(2.5-2.7%)突然升高至2 MAC(6.4-6.6%),在给药前后进行七氟醚诱导的喘息试验。另外3只小鼠分别在给予利鲁唑18 mg/kg前后进行缺氧诱导喘息的试验。随着利鲁唑剂量的增加,七氟醚引起的喘息有明显的剂量依赖性衰减。给予利鲁唑18 mg/kg完全消除缺氧引起的喘息。这些结果表明,七氟醚诱导的喘息,就像缺氧诱导的喘息一样,可以通过激活脑干内持续的钠电流来产生。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Effects of riluzole on sevoflurane-induced gasping in adult mice.

The inhalation of a high sevoflurane concentration under hyperoxia induces gasping, similar to the breathing patterns observed during hypoxia-induced gasping in mice. This observation, coupled with the understanding that burster neurons in the pre-Bötzinger complex, which rely on a persistent sodium current, play a crucial role in generating hypoxia-induced gasping, led us to investigate whether sevoflurane-induced gasping could be triggered by activating this current within the brainstem. To this end, we evaluated the dose-dependent effects of intraperitoneal administration of riluzole, a blocker of persistent sodium channels, on sevoflurane-induced gasping in adult mice. Ten tracheally-intubated, spontaneously breathing, sevoflurane-anesthetized mice. Seven mice randomly received three doses of intraperitoneal riluzole (6, 12, 18 mg/kg, i.p.) with an interval of approximately four weeks. The test trials to elicit the sevoflurane-induced gasping were conducted before and after administering each dose of riluzole by a sudden rise in inspired concentration of sevoflurane from 0.8 MAC (2.5-2.7%) to 2 MAC (6.4-6.6%). In the other three mice, the test trials for eliciting the hypoxia-induced gasping were performed before and after administering 18 mg/kg riluzole. The administration of the increasing dose of riluzole demonstrated an apparent and dose-dependent attenuation of sevoflurane-induced gasping. The administration of 18 mg/kg of riluzole completely abolished the hypoxia-induced gasping. These results indicate that sevoflurane-induced gasping, like hypoxia-induced gasping, could be generated by activating persistent sodium current within the brainstem.

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来源期刊
CiteScore
6.00
自引率
9.10%
发文量
296
审稿时长
2-4 weeks
期刊介绍: The Journal of Applied Physiology publishes the highest quality original research and reviews that examine novel adaptive and integrative physiological mechanisms in humans and animals that advance the field. The journal encourages the submission of manuscripts that examine the acute and adaptive responses of various organs, tissues, cells and/or molecular pathways to environmental, physiological and/or pathophysiological stressors. As an applied physiology journal, topics of interest are not limited to a particular organ system. The journal, therefore, considers a wide array of integrative and translational research topics examining the mechanisms involved in disease processes and mitigation strategies, as well as the promotion of health and well-being throughout the lifespan. Priority is given to manuscripts that provide mechanistic insight deemed to exert an impact on the field.
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