{"title":"利鲁唑对七氟醚诱导的成年小鼠喘息的影响。","authors":"Saki Taiji, Takashi Nishino, Hisayo Jin, Mayumi Hashida, Shiroh Isono","doi":"10.1152/japplphysiol.00347.2025","DOIUrl":null,"url":null,"abstract":"<p><p>The inhalation of a high sevoflurane concentration under hyperoxia induces gasping, similar to the breathing patterns observed during hypoxia-induced gasping in mice. This observation, coupled with the understanding that burster neurons in the pre-Bötzinger complex, which rely on a persistent sodium current, play a crucial role in generating hypoxia-induced gasping, led us to investigate whether sevoflurane-induced gasping could be triggered by activating this current within the brainstem. To this end, we evaluated the dose-dependent effects of intraperitoneal administration of riluzole, a blocker of persistent sodium channels, on sevoflurane-induced gasping in adult mice. Ten tracheally-intubated, spontaneously breathing, sevoflurane-anesthetized mice. Seven mice randomly received three doses of intraperitoneal riluzole (6, 12, 18 mg/kg, i.p.) with an interval of approximately four weeks. The test trials to elicit the sevoflurane-induced gasping were conducted before and after administering each dose of riluzole by a sudden rise in inspired concentration of sevoflurane from 0.8 MAC (2.5-2.7%) to 2 MAC (6.4-6.6%). In the other three mice, the test trials for eliciting the hypoxia-induced gasping were performed before and after administering 18 mg/kg riluzole. The administration of the increasing dose of riluzole demonstrated an apparent and dose-dependent attenuation of sevoflurane-induced gasping. The administration of 18 mg/kg of riluzole completely abolished the hypoxia-induced gasping. These results indicate that sevoflurane-induced gasping, like hypoxia-induced gasping, could be generated by activating persistent sodium current within the brainstem.</p>","PeriodicalId":15160,"journal":{"name":"Journal of applied physiology","volume":" ","pages":""},"PeriodicalIF":3.3000,"publicationDate":"2025-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Effects of riluzole on sevoflurane-induced gasping in adult mice.\",\"authors\":\"Saki Taiji, Takashi Nishino, Hisayo Jin, Mayumi Hashida, Shiroh Isono\",\"doi\":\"10.1152/japplphysiol.00347.2025\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>The inhalation of a high sevoflurane concentration under hyperoxia induces gasping, similar to the breathing patterns observed during hypoxia-induced gasping in mice. This observation, coupled with the understanding that burster neurons in the pre-Bötzinger complex, which rely on a persistent sodium current, play a crucial role in generating hypoxia-induced gasping, led us to investigate whether sevoflurane-induced gasping could be triggered by activating this current within the brainstem. To this end, we evaluated the dose-dependent effects of intraperitoneal administration of riluzole, a blocker of persistent sodium channels, on sevoflurane-induced gasping in adult mice. Ten tracheally-intubated, spontaneously breathing, sevoflurane-anesthetized mice. Seven mice randomly received three doses of intraperitoneal riluzole (6, 12, 18 mg/kg, i.p.) with an interval of approximately four weeks. The test trials to elicit the sevoflurane-induced gasping were conducted before and after administering each dose of riluzole by a sudden rise in inspired concentration of sevoflurane from 0.8 MAC (2.5-2.7%) to 2 MAC (6.4-6.6%). In the other three mice, the test trials for eliciting the hypoxia-induced gasping were performed before and after administering 18 mg/kg riluzole. The administration of the increasing dose of riluzole demonstrated an apparent and dose-dependent attenuation of sevoflurane-induced gasping. The administration of 18 mg/kg of riluzole completely abolished the hypoxia-induced gasping. These results indicate that sevoflurane-induced gasping, like hypoxia-induced gasping, could be generated by activating persistent sodium current within the brainstem.</p>\",\"PeriodicalId\":15160,\"journal\":{\"name\":\"Journal of applied physiology\",\"volume\":\" \",\"pages\":\"\"},\"PeriodicalIF\":3.3000,\"publicationDate\":\"2025-09-27\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of applied physiology\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1152/japplphysiol.00347.2025\",\"RegionNum\":3,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"PHYSIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of applied physiology","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1152/japplphysiol.00347.2025","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"PHYSIOLOGY","Score":null,"Total":0}
Effects of riluzole on sevoflurane-induced gasping in adult mice.
The inhalation of a high sevoflurane concentration under hyperoxia induces gasping, similar to the breathing patterns observed during hypoxia-induced gasping in mice. This observation, coupled with the understanding that burster neurons in the pre-Bötzinger complex, which rely on a persistent sodium current, play a crucial role in generating hypoxia-induced gasping, led us to investigate whether sevoflurane-induced gasping could be triggered by activating this current within the brainstem. To this end, we evaluated the dose-dependent effects of intraperitoneal administration of riluzole, a blocker of persistent sodium channels, on sevoflurane-induced gasping in adult mice. Ten tracheally-intubated, spontaneously breathing, sevoflurane-anesthetized mice. Seven mice randomly received three doses of intraperitoneal riluzole (6, 12, 18 mg/kg, i.p.) with an interval of approximately four weeks. The test trials to elicit the sevoflurane-induced gasping were conducted before and after administering each dose of riluzole by a sudden rise in inspired concentration of sevoflurane from 0.8 MAC (2.5-2.7%) to 2 MAC (6.4-6.6%). In the other three mice, the test trials for eliciting the hypoxia-induced gasping were performed before and after administering 18 mg/kg riluzole. The administration of the increasing dose of riluzole demonstrated an apparent and dose-dependent attenuation of sevoflurane-induced gasping. The administration of 18 mg/kg of riluzole completely abolished the hypoxia-induced gasping. These results indicate that sevoflurane-induced gasping, like hypoxia-induced gasping, could be generated by activating persistent sodium current within the brainstem.
期刊介绍:
The Journal of Applied Physiology publishes the highest quality original research and reviews that examine novel adaptive and integrative physiological mechanisms in humans and animals that advance the field. The journal encourages the submission of manuscripts that examine the acute and adaptive responses of various organs, tissues, cells and/or molecular pathways to environmental, physiological and/or pathophysiological stressors. As an applied physiology journal, topics of interest are not limited to a particular organ system. The journal, therefore, considers a wide array of integrative and translational research topics examining the mechanisms involved in disease processes and mitigation strategies, as well as the promotion of health and well-being throughout the lifespan. Priority is given to manuscripts that provide mechanistic insight deemed to exert an impact on the field.