间歇式球磨强化高固相水稻秸秆酶解

IF 5.8 2区生物学 Q1 AGRICULTURAL ENGINEERING

Biomass & Bioenergy Pub Date : 2025-09-28 DOI:10.1016/j.biombioe.2025.108423

Guanya Ji, Bo Zhang, Bo Feng, Yuchen Xing, Bo Tao, Qijian Niu, Jinxiu Song, Zhong Tang, Haiyan Zhang

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引用次数: 0

摘要

在高干物质条件下，酶解系统的“高固体效应”提出了一个重大挑战，限制了生物质转化的效率。在此，我们探索间歇性球磨作为克服这一障碍的策略的潜力，证明其在增强反应活性，加速反应动力学和减少浪费方面的功效。结果表明，在25% （w/v）高固载条件下，与传统酶解法相比，该方法使秸秆葡萄糖产率在12 h后提高了91.5%。间歇球磨将颗粒尺寸从220.7 μm减小到14.4 μm，提高了基体表面积和纤维素的可及性。我们观察到纤维素酶在高固体酶解系统中的吸附和转移在每次磨球循环的前5分钟内是最有效的，导致12小时后吸附量增加2.9倍。这些发现突出了间歇性球磨提高生物质高固体酶解效率的潜力。

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Enhanced high-solids enzymatic hydrolysis of rice straw by intermittent ball milling

The “high-solids effect” of enzymatic hydrolysis systems at high dry matter presents a significant challenge, limiting the efficiency of biomass conversion. Herein, we explore the potential of intermittent ball milling as a strategy to overcome this hurdle, demonstrating its efficacy in enhancing reactivity, accelerating reaction kinetics, and reducing waste. The results revealed that this method increases glucose yield of rice straw at high-solid loading of 25 % (w/v) by 91.5 % after 12 h, compared to traditional enzymatic hydrolysis. Intermittent ball milling reduces particle size from 220.7 μm to 14.4 μm, enhancing substrate surface area and cellulose accessibility. We observed that cellulase adsorption and transfer in the high-solids enzymatic hydrolysis systems were most effective within the first 5 min of each milling cycle, leading to a 2.9-fold increase in adsorption after 12 h. These findings highlight the potential of intermittent ball milling to enhance high-solids enzymatic hydrolysis efficiency for biomass.

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来源期刊

Biomass & Bioenergy 工程技术-能源与燃料

CiteScore

11.50

自引率

3.30%

发文量

258

审稿时长

60 days

期刊介绍： Biomass & Bioenergy is an international journal publishing original research papers and short communications, review articles and case studies on biological resources, chemical and biological processes, and biomass products for new renewable sources of energy and materials. The scope of the journal extends to the environmental, management and economic aspects of biomass and bioenergy. Key areas covered by the journal: • Biomass: sources, energy crop production processes, genetic improvements, composition. Please note that research on these biomass subjects must be linked directly to bioenergy generation. • Biological Residues: residues/rests from agricultural production, forestry and plantations (palm, sugar etc), processing industries, and municipal sources (MSW). Papers on the use of biomass residues through innovative processes/technological novelty and/or consideration of feedstock/system sustainability (or unsustainability) are welcomed. However waste treatment processes and pollution control or mitigation which are only tangentially related to bioenergy are not in the scope of the journal, as they are more suited to publications in the environmental arena. Papers that describe conventional waste streams (ie well described in existing literature) that do not empirically address ''new'' added value from the process are not suitable for submission to the journal. • Bioenergy Processes: fermentations, thermochemical conversions, liquid and gaseous fuels, and petrochemical substitutes • Bioenergy Utilization: direct combustion, gasification, electricity production, chemical processes, and by-product remediation • Biomass and the Environment: carbon cycle, the net energy efficiency of bioenergy systems, assessment of sustainability, and biodiversity issues.