Research on 5S rDNA, Mitochondria and Nutritional Components of Cambaroides dauricus.

The mitochondrial genome and 5S rDNA are powerful tools for exploring and confirming species identity and understanding evolutionary trajectories. In addition, evaluating the nutritional value of Cambaroides dauricus by determining and analyzing the nutritional components of its muscles could provide essential data for relevant artificial breeding and food processing. In this study, for the first time, we characterized the mitochondrial genome of Cambaroides dauricus using Illumina sequencing technology. The results showed that the mitochondrial genome was a circular genome of 16,215 bp in length. The average sequencing depth of the mitochondrial genome was 100X. The nucleotide composition of the mitochondrial genome was 33.4% A, 39.1% T, 11.0% C and 16.5% G. Phylogenetic analyses showed that Cambaroides dauricus has a very close relationship with Cambaroides wladiwostokiensis. The results of 5S rDNA showed that the genetic relationship between Procambarus clarkii and Cambaroides dauricus is quite close. After determining the nutritional components of Cambaroides dauricus' muscles with normal analysis methods, the results revealed that it has a crude protein content of 18.47 ± 0.87 per 100 milligrams, a crude fat content of 0.83 ± 0.12 per 100 milligrams, a crude ash content of 0.63 ± 0.06 per 100 milligrams and a moisture content of 79.8 ± 0.72 per 100 milligrams. There are eight essential amino acids in its protein, accounting for 41.59% of the total amino acids, and the proportion of umami amino acids is 36.27%. This study will provide a valuable basis for further studies of taxonomy, phylogenetic analyses and artificial breeding in Cambaroides dauricus.