辛伐他汀联合CpG增强H9N2灭活疫苗的免疫原性

IF 2.3 2区 农林科学 Q2 VETERINARY SCIENCES
Yan Ma, Jiaxi Zhu, Zuchen Song, Lina Jiao, Ruihong Yu, Zheng Wang, Zhimin Zhang, Jiaguo Liu, Zhenguang Liu
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引用次数: 0

摘要

H9N2病毒严重损害了畜禽养殖业。目前,主要通过疫苗接种和免疫预防。然而,传统疫苗往往不能诱导持久的免疫反应和持久的免疫保护。本研究以辛伐他汀(Sim)和CpG作为H9N2灭活疫苗的佐剂,对疫苗在鸡体内的免疫原性进行了评价。我们通过抗体测试、T淋巴细胞表型和rna测序分析来评估疫苗的免疫原性。结果表明,Sim + CpG/H9N2配方显著提高特异性IgY和血凝抑制(HI)抗体滴度。增加CD4+ T细胞和CD8+ T细胞的比例,促进免疫器官的发育,刺激生发中心的形成。rna测序分析显示,Sim + CpG/H9N2疫苗接种显著上调了免疫相关基因,这些基因在应激反应激活、免疫细胞募集和炎症信号通路中富集。综上所述,这些发现表明Sim + CpG/H9N2显著增强了灭活H9N2疫苗的免疫原性,为疫苗佐剂的应用提供了新的见解。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Simvastatin Combined with CpG Enhances the Immunogenicity of the H9N2 Inactivated Vaccine.

The H9N2 virus has severely harmed the livestock and bird farming industry. Currently, it is mainly prevented through vaccination immunization. However, conventional vaccines often fail to induce durable immune responses and long-lasting immunoprotection. In this research, we used Simvastatin (Sim) and CpG as adjuvants for the H9N2 inactivated vaccine to evaluate the vaccine's immunogenicity in chickens. We evaluate vaccine immunogenicity through antibody testing, T lymphocyte phenotyping, and RNA-sequencing analysis. The results indicated that the Sim + CpG/H9N2 formulation significantly enhanced specific IgY and hemagglutination inhibition (HI) antibody titers. It also increased the proportions of CD4+ T cells and CD8+ T cells, promoted immune organ development, and stimulated the formation of germinal centers. RNA-sequencing analysis revealed that Sim + CpG/H9N2 vaccination significantly upregulated immune-related genes, which were enriched in pathways associated with stress response activation, immune cell recruitment, and inflammatory signaling. Overall, these findings demonstrate that Sim + CpG/H9N2 markedly enhances the immunogenicity of the inactivated H9N2 vaccine and provides new insights into the application of vaccine adjuvants for improved immune protection.

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来源期刊
Veterinary Sciences
Veterinary Sciences VETERINARY SCIENCES-
CiteScore
2.90
自引率
8.30%
发文量
612
审稿时长
6 weeks
期刊介绍: Veterinary Sciences is an international and interdisciplinary scholarly open access journal. It publishes original that are relevant to any field of veterinary sciences, including prevention, diagnosis and treatment of disease, disorder and injury in animals. This journal covers almost all topics related to animal health and veterinary medicine. Research fields of interest include but are not limited to: anaesthesiology anatomy bacteriology biochemistry cardiology dentistry dermatology embryology endocrinology epidemiology genetics histology immunology microbiology molecular biology mycology neurobiology oncology ophthalmology parasitology pathology pharmacology physiology radiology surgery theriogenology toxicology virology.
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