未经处理和微生物降解的红树林沉积物微塑料对斑马鱼肠道组织学及免疫和抗氧化生物标志物的影响

IF 2.3 2区 农林科学 Q2 VETERINARY SCIENCES
Xin-Yu Zheng, Wan Wei, Asim Muhammad, Min Zhang, Yan-Jun Chen, Jia-Hong Xie, Dan-Ju Kang, Jin-Jun Chen
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引用次数: 0

摘要

MPs是海洋生态系统中普遍存在的污染物,由于其体积小且具有生物蓄积潜力,对水生生物构成风险。本研究研究了从红树林沉积物中提取的MP颗粒对斑马鱼的肠道毒性,比较了微生物降解前后的影响。将斑马鱼暴露于浓度为0(对照)、2、10和50 mg/L的未降解MP或微生物降解MP提取物中21天,在10 L的容器中(放养密度:10鱼/L),每个浓度有3个重复容器。在加入水中之前,对MPs进行超声分散。于第7、14和21天采集肠道标本,分析免疫应答(肿瘤坏死因子-α, TNF-α;白细胞介素-1β, IL-1β;白细胞介素-6,IL-6;白细胞介素-8,IL-8)和抗氧化活性(超氧化物歧化酶,SOD;过氧化氢酶,CAT)。组织病理学分析显示,暴露于未降解和降解的MP提取物的斑马鱼肠壁变薄,绒毛损伤和上皮细胞脱落;然而,未降解的MPs引起更严重的肠道损伤。结果显示细胞因子表达的动态变化:TNF-α先下降后升高,IL-1β和IL-8先上升后下降。IL-6在第7天达到峰值,第14天下降,第21天再次升高。仅降解前组CAT表达降低,SOD表达升高。微生物降解降低了肠道损伤的严重程度,其影响在MP暴露水平较高时加剧。这些发现表明,MPs可以损害斑马鱼的消化系统,但微生物降解减轻其毒性。本研究强调了生物降解作为一种潜在的环境修复策略的重要性,并为MPs对水生生物的影响提供了实验证据。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Influence of Untreated and Microbially Degraded Mangrove Sediment Microplastics on Zebrafish (Danio rerio) Intestinal Histology and Immune and Antioxidant Biomarkers.

MPs are pervasive pollutants in marine ecosystems, posing risks to aquatic organisms due to their small size and bioaccumulation potential. This study investigated the intestinal toxicity of MP particles extracted from mangrove sediments in zebrafish, comparing the effects before and after microbial degradation. Zebrafish were exposed to either undegraded MPs or microbially degraded MP extracts at concentrations of 0 (control), 2, 10, and 50 mg/L for 21 days in 10 L tanks (stocking density: 10 fish/L), with three replicate tanks per concentration. MPs were dispersed ultrasonically before addition to the water. Intestinal samples were collected on 7, 14, and 21 days for the analysis of immune response (tumor necrosis factor-alpha, TNF-α; interleukin-1 beta, IL-1β; interleukin-6, IL-6; interleukin-8, IL-8) and antioxidant activity (superoxide dismutase, SOD; catalase, CAT). Histopathological analysis revealed intestinal wall thinning, villus damage, and epithelial cell detachment in zebrafish exposed to both undegraded and degraded MP extracts; however, undegraded MPs induced more severe intestinal damage. Results indicated dynamic changes in cytokine expression: TNF-α decreased initially before increasing, while IL-1β and IL-8 first rose then declined. IL-6 peaked on day 7, dropped by day 14, and increased again on day 21. CAT expression decreased, whereas SOD increased only in the pre-degradation group. Microbial degradation reduced intestinal damage severity, with effects intensifying at higher MP exposure levels. These findings demonstrate that MPs can impair zebrafish digestive systems, but microbial degradation mitigates their toxicity. This study underscores the importance of biodegradation as a potential environmental remediation strategy and provides experimental evidence on MPs' impact on aquatic organisms.

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来源期刊
Veterinary Sciences
Veterinary Sciences VETERINARY SCIENCES-
CiteScore
2.90
自引率
8.30%
发文量
612
审稿时长
6 weeks
期刊介绍: Veterinary Sciences is an international and interdisciplinary scholarly open access journal. It publishes original that are relevant to any field of veterinary sciences, including prevention, diagnosis and treatment of disease, disorder and injury in animals. This journal covers almost all topics related to animal health and veterinary medicine. Research fields of interest include but are not limited to: anaesthesiology anatomy bacteriology biochemistry cardiology dentistry dermatology embryology endocrinology epidemiology genetics histology immunology microbiology molecular biology mycology neurobiology oncology ophthalmology parasitology pathology pharmacology physiology radiology surgery theriogenology toxicology virology.
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