Bacterial Tolerance and Bioleaching in the Presence of Chloride.

Chloride ions can enhance the bioleaching of copper minerals, yet most biomining microorganisms are highly sensitive to chloride and cannot survive or colonize mineral surfaces in saline environments. Chloride tolerance varies among acidophilic iron-oxidizing bacteria, but the concentrations at which they remain active are generally too low to permit the industrial use of seawater. Therefore, identifying highly chloride-tolerant leaching microorganisms and studying their bioleaching potential in chloride-containing systems is of utmost importance. This study investigated chloride tolerance and adaptability of bacteria from different genera, with a focus on Sulfobacillus thermosulfidooxidans subsp. asporogenes 41, a moderately thermophilic strain that can oxidize both Fe (II) and reduced inorganic sulfur compounds (RISCs). This dual activity makes it advantageous for bioleaching by facilitating sulfur removal, generating acidity, and preventing mineral passivation. Comparative experiments on the bioleaching of pyrite and chalcopyrite demonstrated that adaptation to 0.3 M NaCl enhanced the chloride tolerance of S. thermosulfidooxidans subsp. asporogenes 41. The adapted strain exhibited significantly improved copper extraction under saline conditions compared with the native culture. Maximum copper recovery was achieved at 0.4 M NaCl, highlighting the potential of chloride-adapted moderate thermophiles for biomining applications in saline environments. In contrast the minimal inhibitory concentration for Acidithiobacillud ferrooxidans Dr was 0.005 M (causing 41.2% inhibition), while Leptospirillum ferriphilum CC was unaffected by lower concentrations (0.01-0.02 M) and only showed severe inhibition (86.5%) at 0.1 M NaCl, defining its minimal inhibitory concentration (MIC) at 0.05 M.