维吉尼亚皂苷A：一种新的维吉尼亚甾体生物碱皂苷的分离、结构解析及抗脂肪活性。

IF 2.6 3区医学 Q3 CHEMISTRY, MEDICINAL

Fitoterapia Pub Date : 2025-09-24 DOI:10.1016/j.fitote.2025.106897

Anuradha Vishwakarma , Jay Gupta , Astha Singh , Anil Nilkanth Gaikwad , Koneni V. Sashidhara

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引用次数: 0

摘要

一种以前未报道的16β-H螺旋体皂苷，维吉尼亚皂苷A(1)，以及三种已知化合物；从维吉尼亚茄（Solanum virginanum）全株中分离得到薯蓣皂苷元（dioosgenin, 2）、solasodine （solasodine, 3）和proapogenin B （proapogenin, 4）。通过详细的ROESY和HMBC分析，建立了罕见的1的16β-H构型。所有化合物都被评估为抗脂肪生成活性，其中1和2显示显著减少3 T3-L1细胞系的脂质积累。维吉尼亚苷A(1)是最有效的，表现出剂量依赖性的活性，抑制PPARγ、C/EBPα、FASN和FABP4的表达，无明显的细胞毒性，表明其在脂肪细胞分化的早期阶段起作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Virginianoside A, a new steroidal alkaloid saponin from Solanum virginianum: Isolation, structure elucidation and anti-adipogenic activity

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Virginianoside A, a new steroidal alkaloid saponin from Solanum virginianum: Isolation, structure elucidation and anti-adipogenic activity

A previously unreported 16β-H spirosolane saponin, virginianoside A (1), along with three known compounds; diosgenin (2), solasodine (3) and prosapogenin B (4) were isolated from the whole plant of Solanum virginianum. The rare 16β-H configuration of 1 was established through detailed ROESY and HMBC analysis. All compounds were evaluated for anti-adipogenic activity, with 1 and 2 showing significant reduction in lipid accumulation on 3T3-L1 cell line. Virginianoside A (1) was the most potent, exhibiting dose-dependent activity, suppressing the expression of PPARγ, C/EBPα, FASN and FABP4, without notable cytotoxicity indicating its action during the early phase of adipocyte differentiation.

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来源期刊

Fitoterapia 医学-药学

CiteScore

5.80

自引率

2.90%

发文量

198

审稿时长

1.5 months

期刊介绍： Fitoterapia is a Journal dedicated to medicinal plants and to bioactive natural products of plant origin. It publishes original contributions in seven major areas: 1. Characterization of active ingredients of medicinal plants 2. Development of standardization method for bioactive plant extracts and natural products 3. Identification of bioactivity in plant extracts 4. Identification of targets and mechanism of activity of plant extracts 5. Production and genomic characterization of medicinal plants biomass 6. Chemistry and biochemistry of bioactive natural products of plant origin 7. Critical reviews of the historical, clinical and legal status of medicinal plants, and accounts on topical issues.