Hongxi Chen , Guixuan Chen , Zhihao Chen , Xiaowen Lu , Shirong Fu , Weijiang Liao , Yu Jiao , Qingheng Wang , Chuangye Yang , Yuewen Deng
{"title":"Rel基因的两个同工型参与马氏珠贝的免疫调节。","authors":"Hongxi Chen , Guixuan Chen , Zhihao Chen , Xiaowen Lu , Shirong Fu , Weijiang Liao , Yu Jiao , Qingheng Wang , Chuangye Yang , Yuewen Deng","doi":"10.1016/j.fsi.2025.110895","DOIUrl":null,"url":null,"abstract":"<div><div>Rel gene from pearl oyster <em>Pinctada fucata martensii</em> (Pm-Rel) belongs to the nuclear factor kappa B (NF-κB) gene family and involved in the immune regulation in pearl oyster. In this study, two isoforms of Pm-Rel (Pm-Rel349 and Pm-Rel418) were verified and functionally analyzed. Sequence analysis revealed that Pm-Rel349 and Pm-Rel418 were produced by the exon-deleted and intron-inserted, respectively. Pm-Rel349 lost the C-terminal region, and retained the N-terminal RHD-DNA bind and RHD-dimer region, while Pm-Rel418 lost the N-terminal region, and contained partial RHD-DNA bind, IPT domain and C-terminal TAD region. Both of them were significantly up-regulated at 24 h after transplantation and localized in the cytoplasm and nucleus, with mainly localization in the cytoplasm. Over-expression of Pm-Rel349 and Pm-Rel418 could activate the NF-κB signal pathway, and co-high-expression of both genes yields a stronger activation effect than individual high expression, indicating Pm-Rel349 and Pm-Rel418 have a synergistic effect, and bimolecular fluorescence complementation assay confirmed the interaction between Pm-Rel349 and Pm-Rel418. These findings suggest that Pm-Rel349 and Pm-Rel418 are crucial regulators of immune responses in <em>P</em>. <em>f</em>. <em>martensii</em>.</div></div>","PeriodicalId":12127,"journal":{"name":"Fish & shellfish immunology","volume":"167 ","pages":"Article 110895"},"PeriodicalIF":3.9000,"publicationDate":"2025-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Two isoforms of Rel gene involved in the immune regulation of pearl oyster Pinctada fucata martensii\",\"authors\":\"Hongxi Chen , Guixuan Chen , Zhihao Chen , Xiaowen Lu , Shirong Fu , Weijiang Liao , Yu Jiao , Qingheng Wang , Chuangye Yang , Yuewen Deng\",\"doi\":\"10.1016/j.fsi.2025.110895\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>Rel gene from pearl oyster <em>Pinctada fucata martensii</em> (Pm-Rel) belongs to the nuclear factor kappa B (NF-κB) gene family and involved in the immune regulation in pearl oyster. In this study, two isoforms of Pm-Rel (Pm-Rel349 and Pm-Rel418) were verified and functionally analyzed. Sequence analysis revealed that Pm-Rel349 and Pm-Rel418 were produced by the exon-deleted and intron-inserted, respectively. Pm-Rel349 lost the C-terminal region, and retained the N-terminal RHD-DNA bind and RHD-dimer region, while Pm-Rel418 lost the N-terminal region, and contained partial RHD-DNA bind, IPT domain and C-terminal TAD region. Both of them were significantly up-regulated at 24 h after transplantation and localized in the cytoplasm and nucleus, with mainly localization in the cytoplasm. Over-expression of Pm-Rel349 and Pm-Rel418 could activate the NF-κB signal pathway, and co-high-expression of both genes yields a stronger activation effect than individual high expression, indicating Pm-Rel349 and Pm-Rel418 have a synergistic effect, and bimolecular fluorescence complementation assay confirmed the interaction between Pm-Rel349 and Pm-Rel418. These findings suggest that Pm-Rel349 and Pm-Rel418 are crucial regulators of immune responses in <em>P</em>. <em>f</em>. <em>martensii</em>.</div></div>\",\"PeriodicalId\":12127,\"journal\":{\"name\":\"Fish & shellfish immunology\",\"volume\":\"167 \",\"pages\":\"Article 110895\"},\"PeriodicalIF\":3.9000,\"publicationDate\":\"2025-09-24\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Fish & shellfish immunology\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S1050464825007843\",\"RegionNum\":2,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"FISHERIES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Fish & shellfish immunology","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1050464825007843","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"FISHERIES","Score":null,"Total":0}
Two isoforms of Rel gene involved in the immune regulation of pearl oyster Pinctada fucata martensii
Rel gene from pearl oyster Pinctada fucata martensii (Pm-Rel) belongs to the nuclear factor kappa B (NF-κB) gene family and involved in the immune regulation in pearl oyster. In this study, two isoforms of Pm-Rel (Pm-Rel349 and Pm-Rel418) were verified and functionally analyzed. Sequence analysis revealed that Pm-Rel349 and Pm-Rel418 were produced by the exon-deleted and intron-inserted, respectively. Pm-Rel349 lost the C-terminal region, and retained the N-terminal RHD-DNA bind and RHD-dimer region, while Pm-Rel418 lost the N-terminal region, and contained partial RHD-DNA bind, IPT domain and C-terminal TAD region. Both of them were significantly up-regulated at 24 h after transplantation and localized in the cytoplasm and nucleus, with mainly localization in the cytoplasm. Over-expression of Pm-Rel349 and Pm-Rel418 could activate the NF-κB signal pathway, and co-high-expression of both genes yields a stronger activation effect than individual high expression, indicating Pm-Rel349 and Pm-Rel418 have a synergistic effect, and bimolecular fluorescence complementation assay confirmed the interaction between Pm-Rel349 and Pm-Rel418. These findings suggest that Pm-Rel349 and Pm-Rel418 are crucial regulators of immune responses in P. f. martensii.
期刊介绍:
Fish and Shellfish Immunology rapidly publishes high-quality, peer-refereed contributions in the expanding fields of fish and shellfish immunology. It presents studies on the basic mechanisms of both the specific and non-specific defense systems, the cells, tissues, and humoral factors involved, their dependence on environmental and intrinsic factors, response to pathogens, response to vaccination, and applied studies on the development of specific vaccines for use in the aquaculture industry.