Rel基因的两个同工型参与马氏珠贝的免疫调节。

IF 3.9 2区 农林科学 Q1 FISHERIES
Hongxi Chen , Guixuan Chen , Zhihao Chen , Xiaowen Lu , Shirong Fu , Weijiang Liao , Yu Jiao , Qingheng Wang , Chuangye Yang , Yuewen Deng
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引用次数: 0

摘要

来自fucata martensii珍珠贝的Rel基因(pmrel)属于核因子κB (NF-κB)基因家族,参与珍珠贝的免疫调节。本研究对Pm-Rel的两个同工异构体(Pm-Rel349和Pm-Rel418)进行了验证和功能分析。序列分析显示,Pm-Rel349和Pm-Rel418分别由外显子缺失和内含子插入产生。Pm-Rel349缺失了c端区域,保留了n端RHD-DNA结合和rhd -二聚体区域,而Pm-Rel418缺失了n端区域,包含部分RHD-DNA结合、IPT结构域和c端TAD区域。两者在移植后24 h均显著上调,定位于细胞质和细胞核,主要定位于细胞质。Pm-Rel349和Pm-Rel418过表达可激活NF-κB信号通路,且两者共同高表达比单独高表达的激活作用更强,说明Pm-Rel349和Pm-Rel418具有协同作用,双分子荧光互补实验证实Pm-Rel349与Pm-Rel418之间存在相互作用。这些发现表明,Pm-Rel349和Pm-Rel418是马氏p.f . martensii免疫应答的重要调节因子。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Two isoforms of Rel gene involved in the immune regulation of pearl oyster Pinctada fucata martensii
Rel gene from pearl oyster Pinctada fucata martensii (Pm-Rel) belongs to the nuclear factor kappa B (NF-κB) gene family and involved in the immune regulation in pearl oyster. In this study, two isoforms of Pm-Rel (Pm-Rel349 and Pm-Rel418) were verified and functionally analyzed. Sequence analysis revealed that Pm-Rel349 and Pm-Rel418 were produced by the exon-deleted and intron-inserted, respectively. Pm-Rel349 lost the C-terminal region, and retained the N-terminal RHD-DNA bind and RHD-dimer region, while Pm-Rel418 lost the N-terminal region, and contained partial RHD-DNA bind, IPT domain and C-terminal TAD region. Both of them were significantly up-regulated at 24 h after transplantation and localized in the cytoplasm and nucleus, with mainly localization in the cytoplasm. Over-expression of Pm-Rel349 and Pm-Rel418 could activate the NF-κB signal pathway, and co-high-expression of both genes yields a stronger activation effect than individual high expression, indicating Pm-Rel349 and Pm-Rel418 have a synergistic effect, and bimolecular fluorescence complementation assay confirmed the interaction between Pm-Rel349 and Pm-Rel418. These findings suggest that Pm-Rel349 and Pm-Rel418 are crucial regulators of immune responses in P. f. martensii.
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来源期刊
Fish & shellfish immunology
Fish & shellfish immunology 农林科学-海洋与淡水生物学
CiteScore
7.50
自引率
19.10%
发文量
750
审稿时长
68 days
期刊介绍: Fish and Shellfish Immunology rapidly publishes high-quality, peer-refereed contributions in the expanding fields of fish and shellfish immunology. It presents studies on the basic mechanisms of both the specific and non-specific defense systems, the cells, tissues, and humoral factors involved, their dependence on environmental and intrinsic factors, response to pathogens, response to vaccination, and applied studies on the development of specific vaccines for use in the aquaculture industry.
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