地中海嗜盐古菌聚羟基烷酸合成酶的纯化与表征：生物降解塑料合成关键酶。

IF 3.7 3区医学 Q2 ENGINEERING, BIOMEDICAL

Bioengineering Pub Date : 2025-09-22 DOI:10.3390/bioengineering12091003

Diya Alsafadi, Yomen Ghalawinji, Fawwaz I Khalili

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引用次数: 0

摘要

聚羟基烷酸酯（PHA）生物聚合物的生物合成高度依赖于关键酶PHA合成酶（PhaC）的活性。地中海嗜盐古菌在非灭菌条件下可从不同碳源积累大量pha。在这项研究中，从地中海H.产生了一种PhaC酶，随后用离子交换色谱法部分纯化。SDS-PAGE显示该蛋白的亚基分子质量为56.4 kDa。纯化酶将羟基丁基辅酶a分子转化为PHA，在pH 10.0和pH 8.0时具有最佳活性。PhaC在30 ~ 70℃范围内具有热活性，在50℃时具有最大活性。经证实，该酶具有亲卤碱性（在pH 7.0和高盐浓度下具有活性），在25°C下24小时具有一定程度的稳定性。

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Purification and Characterization of Polyhydroxyalkanoate Synthase from Extremely Halophilic Archaeon Haloferax mediterranei: Key Enzyme of Biodegradable Plastic Synthesis.

The biosynthesis of polyhydroxyalkanoate (PHA) biopolymer is highly dependent on the activity of a key enzyme, PHA synthase (PhaC). The halophilic archaeon Haloferax mediterranei can accumulate large amounts of PHAs from different carbon sources under non-sterilized conditions. In this study, a PhaC enzyme from H. mediterranei was produced and subsequently partially purified by ion exchange chromatography. The protein was visualized by SDS-PAGE, with a subunit molecular mass of 56.4 kDa. The purified enzyme converts hydroxybutyryl CoA molecules into PHA, being optimally active at pH 10.0 and pH 8.0. The PhaC was thermoactive in the range of 30 °C to 70 °C, with maximum activity registered at 50 °C. The enzyme was confirmed to be haloalkaliphilic (active at pH > 7.0 and high salt concentration) and exhibit a degree of stability at 25 °C for 24 h.

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来源期刊

Bioengineering Chemical Engineering-Bioengineering

CiteScore

4.00

自引率

8.70%

发文量

661

期刊介绍： Aims Bioengineering (ISSN 2306-5354) provides an advanced forum for the science and technology of bioengineering. It publishes original research papers, comprehensive reviews, communications and case reports. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. All aspects of bioengineering are welcomed from theoretical concepts to education and applications. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. There are, in addition, four key features of this Journal: ● We are introducing a new concept in scientific and technical publications “The Translational Case Report in Bioengineering”. It is a descriptive explanatory analysis of a transformative or translational event. Understanding that the goal of bioengineering scholarship is to advance towards a transformative or clinical solution to an identified transformative/clinical need, the translational case report is used to explore causation in order to find underlying principles that may guide other similar transformative/translational undertakings. ● Manuscripts regarding research proposals and research ideas will be particularly welcomed. ● Electronic files and software regarding the full details of the calculation and experimental procedure, if unable to be published in a normal way, can be deposited as supplementary material. ● We also accept manuscripts communicating to a broader audience with regard to research projects financed with public funds. Scope ● Bionics and biological cybernetics: implantology; bio–abio interfaces ● Bioelectronics: wearable electronics; implantable electronics; “more than Moore” electronics; bioelectronics devices ● Bioprocess and biosystems engineering and applications: bioprocess design; biocatalysis; bioseparation and bioreactors; bioinformatics; bioenergy; etc. ● Biomolecular, cellular and tissue engineering and applications: tissue engineering; chromosome engineering; embryo engineering; cellular, molecular and synthetic biology; metabolic engineering; bio-nanotechnology; micro/nano technologies; genetic engineering; transgenic technology ● Biomedical engineering and applications: biomechatronics; biomedical electronics; biomechanics; biomaterials; biomimetics; biomedical diagnostics; biomedical therapy; biomedical devices; sensors and circuits; biomedical imaging and medical information systems; implants and regenerative medicine; neurotechnology; clinical engineering; rehabilitation engineering ● Biochemical engineering and applications: metabolic pathway engineering; modeling and simulation ● Translational bioengineering