Monier Alhadi Abdelrahman Mohammed, Zhenbin Chen, Mohammed Kamal Hadi
{"title":"海藻酸钠/聚乙二醇/聚乙烯醇聚合物掺杂Mn2+包被再生Fe3O4颗粒载体提高固定化青霉素G酰化酶的催化活性和可重复利用性的研究","authors":"Monier Alhadi Abdelrahman Mohammed, Zhenbin Chen, Mohammed Kamal Hadi","doi":"10.1007/s10924-025-03603-7","DOIUrl":null,"url":null,"abstract":"<div><p>In this study, Fe<sub>3</sub>O<sub>4</sub> magnetic particles (MPs) were extracted from nickel slag using molten oxidation and magnetic separation, then coated with a blend of sodium alginate (SA)/polyethylene glycol (PEG)/polyvinyl alcohol (PVA) and doped with Mn²⁺ to form Mn²⁺-Fe<sub>3</sub>O<sub>4</sub>@SA/PEG/PVA MPs. Glutaraldehyde (GA) was grafted onto these particles to obtain Mn²⁺-Fe<sub>3</sub>O<sub>4</sub>@SA/PEG/PVA-GA MPs, used as carriers for Penicillin G acylase (PGA) immobilization through a Schiff base reaction. Analytical approaches such as fourier transform infrared spectroscopy (FTIR), x-ray diffraction (XRD), vibrating sample magnetometer (VSM), scanning electron microscope-energy spectroscopy of dispersive x-rays (SEM-EDS), inductively coupled plasma mass spectrometry (ICP-MS) confirmed each modification stage. Enzymological properties of free and immobilized PGA were investigated. The findings have been achieved employing a 2.5 vol% of enzyme solution concentration, a pH of 8.0, an immobilization time of 24 h, and an immobilization temperature of 40 °C. Under these circumstances, the immobilized PGA exhibited enhanced stability across various pH and temperature settings, attaining an enzyme activity retention (<i>EAR</i>) of 95.6%, an enzyme activity (<i>EA</i>) of 31,996 U/g, and an enzyme loading capacity (<i>ELC</i>) of 118 mg/g. After 15 uses, immobilized PGA retained 92% of its initial activity, and the carrier recovery (<i>Re</i>) reached 98%. Immobilized PGA displayed superior operational and storage stability, making it highly promising for practical applications due to its increased efficiency and extended lifetime.</p><h3>Graphical Abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":659,"journal":{"name":"Journal of Polymers and the Environment","volume":"33 7","pages":"3348 - 3367"},"PeriodicalIF":5.0000,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Study of Sodium Alginate/polyethylene Glycol/polyvinyl Alcohol Polymeric, Doped Mn2+, Coated Recycled Fe3O4 Particle Carriers for Enhancing Catalytic Activity and Reusability of Immobilization Penicillin G Acylase\",\"authors\":\"Monier Alhadi Abdelrahman Mohammed, Zhenbin Chen, Mohammed Kamal Hadi\",\"doi\":\"10.1007/s10924-025-03603-7\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>In this study, Fe<sub>3</sub>O<sub>4</sub> magnetic particles (MPs) were extracted from nickel slag using molten oxidation and magnetic separation, then coated with a blend of sodium alginate (SA)/polyethylene glycol (PEG)/polyvinyl alcohol (PVA) and doped with Mn²⁺ to form Mn²⁺-Fe<sub>3</sub>O<sub>4</sub>@SA/PEG/PVA MPs. Glutaraldehyde (GA) was grafted onto these particles to obtain Mn²⁺-Fe<sub>3</sub>O<sub>4</sub>@SA/PEG/PVA-GA MPs, used as carriers for Penicillin G acylase (PGA) immobilization through a Schiff base reaction. Analytical approaches such as fourier transform infrared spectroscopy (FTIR), x-ray diffraction (XRD), vibrating sample magnetometer (VSM), scanning electron microscope-energy spectroscopy of dispersive x-rays (SEM-EDS), inductively coupled plasma mass spectrometry (ICP-MS) confirmed each modification stage. Enzymological properties of free and immobilized PGA were investigated. The findings have been achieved employing a 2.5 vol% of enzyme solution concentration, a pH of 8.0, an immobilization time of 24 h, and an immobilization temperature of 40 °C. Under these circumstances, the immobilized PGA exhibited enhanced stability across various pH and temperature settings, attaining an enzyme activity retention (<i>EAR</i>) of 95.6%, an enzyme activity (<i>EA</i>) of 31,996 U/g, and an enzyme loading capacity (<i>ELC</i>) of 118 mg/g. After 15 uses, immobilized PGA retained 92% of its initial activity, and the carrier recovery (<i>Re</i>) reached 98%. Immobilized PGA displayed superior operational and storage stability, making it highly promising for practical applications due to its increased efficiency and extended lifetime.</p><h3>Graphical Abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>\",\"PeriodicalId\":659,\"journal\":{\"name\":\"Journal of Polymers and the Environment\",\"volume\":\"33 7\",\"pages\":\"3348 - 3367\"},\"PeriodicalIF\":5.0000,\"publicationDate\":\"2025-05-29\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Polymers and the Environment\",\"FirstCategoryId\":\"5\",\"ListUrlMain\":\"https://link.springer.com/article/10.1007/s10924-025-03603-7\",\"RegionNum\":3,\"RegionCategory\":\"工程技术\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"ENGINEERING, ENVIRONMENTAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Polymers and the Environment","FirstCategoryId":"5","ListUrlMain":"https://link.springer.com/article/10.1007/s10924-025-03603-7","RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"ENGINEERING, ENVIRONMENTAL","Score":null,"Total":0}
Study of Sodium Alginate/polyethylene Glycol/polyvinyl Alcohol Polymeric, Doped Mn2+, Coated Recycled Fe3O4 Particle Carriers for Enhancing Catalytic Activity and Reusability of Immobilization Penicillin G Acylase
In this study, Fe3O4 magnetic particles (MPs) were extracted from nickel slag using molten oxidation and magnetic separation, then coated with a blend of sodium alginate (SA)/polyethylene glycol (PEG)/polyvinyl alcohol (PVA) and doped with Mn²⁺ to form Mn²⁺-Fe3O4@SA/PEG/PVA MPs. Glutaraldehyde (GA) was grafted onto these particles to obtain Mn²⁺-Fe3O4@SA/PEG/PVA-GA MPs, used as carriers for Penicillin G acylase (PGA) immobilization through a Schiff base reaction. Analytical approaches such as fourier transform infrared spectroscopy (FTIR), x-ray diffraction (XRD), vibrating sample magnetometer (VSM), scanning electron microscope-energy spectroscopy of dispersive x-rays (SEM-EDS), inductively coupled plasma mass spectrometry (ICP-MS) confirmed each modification stage. Enzymological properties of free and immobilized PGA were investigated. The findings have been achieved employing a 2.5 vol% of enzyme solution concentration, a pH of 8.0, an immobilization time of 24 h, and an immobilization temperature of 40 °C. Under these circumstances, the immobilized PGA exhibited enhanced stability across various pH and temperature settings, attaining an enzyme activity retention (EAR) of 95.6%, an enzyme activity (EA) of 31,996 U/g, and an enzyme loading capacity (ELC) of 118 mg/g. After 15 uses, immobilized PGA retained 92% of its initial activity, and the carrier recovery (Re) reached 98%. Immobilized PGA displayed superior operational and storage stability, making it highly promising for practical applications due to its increased efficiency and extended lifetime.
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The Journal of Polymers and the Environment fills the need for an international forum in this diverse and rapidly expanding field. The journal serves a crucial role for the publication of information from a wide range of disciplines and is a central outlet for the publication of high-quality peer-reviewed original papers, review articles and short communications. The journal is intentionally interdisciplinary in regard to contributions and covers the following subjects - polymers, environmentally degradable polymers, and degradation pathways: biological, photochemical, oxidative and hydrolytic; new environmental materials: derived by chemical and biosynthetic routes; environmental blends and composites; developments in processing and reactive processing of environmental polymers; characterization of environmental materials: mechanical, physical, thermal, rheological, morphological, and others; recyclable polymers and plastics recycling environmental testing: in-laboratory simulations, outdoor exposures, and standardization of methodologies; environmental fate: end products and intermediates of biodegradation; microbiology and enzymology of polymer biodegradation; solid-waste management and public legislation specific to environmental polymers; and other related topics.
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