Mai El Halawany, Heba Ahmed Saleh, Mohammed Khashaba, Mohamed H. H. AbouGhaly, Randa Latif
{"title":"载氨甲环酸海藻酸酯支架对兔骨形成的影响：止血和组织形态学分析","authors":"Mai El Halawany, Heba Ahmed Saleh, Mohammed Khashaba, Mohamed H. H. AbouGhaly, Randa Latif","doi":"10.1186/s43094-025-00849-9","DOIUrl":null,"url":null,"abstract":"<div><h3>Background</h3><p>Bone tissue regeneration based on the use of porous biomaterial scaffolds is considered a promising approach for treating bone defects and fractures healing. A porous alginate scaffold comprising hydroxyapatite nanoparticles loaded with tranexamic acid was formulated. The prepared scaffolds were characterized in terms of the release profile of tranexamic acid and scanning electron microscopy imaging. A cranial bone defect in rabbits (6 defects/3 rabbits/group) was used as a model for the assessment of hemostatic activity of the used scaffolds and the assessment of the bone formation histomorphometrically after its application for 14 days.</p><h3>Results</h3><p>The scaffold appeared with irregular porous structure and controlled the release of tranexamic acid over 4 h. The hemostatic time of the medicated and non-medicated scaffolds were 20 and 60 s, respectively. They were significantly lower than the control group (200 s, <i>p</i> < 0.05). The microscopic examination was done after staining histologically prepared sections from the bone defect with Masson trichrome stain and the area % of the newly developed bone was computed. For the medicated group, the new bone area % (75.8 ± 4.9%) was significantly higher than the non-medicated group (58.1 ± 5.9%, <i>p</i> < 0.001). Both groups were significantly larger than the control group that showed bone area % of 43.1 ± 5.6 (<i>p</i> < 0.05). The histomorphometric analysis showed that the medicated scaffold-treated group had more mineralized newly formed bone tissue and smaller amount of soft tissue and residual materials. In contrast, the non-medicated scaffold showed non-mineralized bone cells with larger soft tissue and residual materials.</p><h3>Conclusion</h3><p>These results suggested the promising effect of the tranexamic acid-loaded scaffolds in minimizing the time to reach hemostasis by stabilization of the formed hematoma. Additionally, they could improve the quality (mineralization) and the quantity (amount) of the newly formed bone.</p><h3>Graphical abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":577,"journal":{"name":"Future Journal of Pharmaceutical Sciences","volume":"11 1","pages":""},"PeriodicalIF":3.0000,"publicationDate":"2025-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://fjps.springeropen.com/counter/pdf/10.1186/s43094-025-00849-9","citationCount":"0","resultStr":"{\"title\":\"The effect of tranexamic acid-loaded alginate scaffolds on bone formation: hemostatic and histomorphometric analysis in a rabbit model\",\"authors\":\"Mai El Halawany, Heba Ahmed Saleh, Mohammed Khashaba, Mohamed H. H. AbouGhaly, Randa Latif\",\"doi\":\"10.1186/s43094-025-00849-9\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Background</h3><p>Bone tissue regeneration based on the use of porous biomaterial scaffolds is considered a promising approach for treating bone defects and fractures healing. A porous alginate scaffold comprising hydroxyapatite nanoparticles loaded with tranexamic acid was formulated. The prepared scaffolds were characterized in terms of the release profile of tranexamic acid and scanning electron microscopy imaging. A cranial bone defect in rabbits (6 defects/3 rabbits/group) was used as a model for the assessment of hemostatic activity of the used scaffolds and the assessment of the bone formation histomorphometrically after its application for 14 days.</p><h3>Results</h3><p>The scaffold appeared with irregular porous structure and controlled the release of tranexamic acid over 4 h. The hemostatic time of the medicated and non-medicated scaffolds were 20 and 60 s, respectively. They were significantly lower than the control group (200 s, <i>p</i> < 0.05). The microscopic examination was done after staining histologically prepared sections from the bone defect with Masson trichrome stain and the area % of the newly developed bone was computed. For the medicated group, the new bone area % (75.8 ± 4.9%) was significantly higher than the non-medicated group (58.1 ± 5.9%, <i>p</i> < 0.001). Both groups were significantly larger than the control group that showed bone area % of 43.1 ± 5.6 (<i>p</i> < 0.05). The histomorphometric analysis showed that the medicated scaffold-treated group had more mineralized newly formed bone tissue and smaller amount of soft tissue and residual materials. In contrast, the non-medicated scaffold showed non-mineralized bone cells with larger soft tissue and residual materials.</p><h3>Conclusion</h3><p>These results suggested the promising effect of the tranexamic acid-loaded scaffolds in minimizing the time to reach hemostasis by stabilization of the formed hematoma. 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The effect of tranexamic acid-loaded alginate scaffolds on bone formation: hemostatic and histomorphometric analysis in a rabbit model
Background
Bone tissue regeneration based on the use of porous biomaterial scaffolds is considered a promising approach for treating bone defects and fractures healing. A porous alginate scaffold comprising hydroxyapatite nanoparticles loaded with tranexamic acid was formulated. The prepared scaffolds were characterized in terms of the release profile of tranexamic acid and scanning electron microscopy imaging. A cranial bone defect in rabbits (6 defects/3 rabbits/group) was used as a model for the assessment of hemostatic activity of the used scaffolds and the assessment of the bone formation histomorphometrically after its application for 14 days.
Results
The scaffold appeared with irregular porous structure and controlled the release of tranexamic acid over 4 h. The hemostatic time of the medicated and non-medicated scaffolds were 20 and 60 s, respectively. They were significantly lower than the control group (200 s, p < 0.05). The microscopic examination was done after staining histologically prepared sections from the bone defect with Masson trichrome stain and the area % of the newly developed bone was computed. For the medicated group, the new bone area % (75.8 ± 4.9%) was significantly higher than the non-medicated group (58.1 ± 5.9%, p < 0.001). Both groups were significantly larger than the control group that showed bone area % of 43.1 ± 5.6 (p < 0.05). The histomorphometric analysis showed that the medicated scaffold-treated group had more mineralized newly formed bone tissue and smaller amount of soft tissue and residual materials. In contrast, the non-medicated scaffold showed non-mineralized bone cells with larger soft tissue and residual materials.
Conclusion
These results suggested the promising effect of the tranexamic acid-loaded scaffolds in minimizing the time to reach hemostasis by stabilization of the formed hematoma. Additionally, they could improve the quality (mineralization) and the quantity (amount) of the newly formed bone.
期刊介绍：
Future Journal of Pharmaceutical Sciences (FJPS) is the official journal of the Future University in Egypt. It is a peer-reviewed, open access journal which publishes original research articles, review articles and case studies on all aspects of pharmaceutical sciences and technologies, pharmacy practice and related clinical aspects, and pharmacy education. The journal publishes articles covering developments in drug absorption and metabolism, pharmacokinetics and dynamics, drug delivery systems, drug targeting and nano-technology. It also covers development of new systems, methods and techniques in pharmacy education and practice. The scope of the journal also extends to cover advancements in toxicology, cell and molecular biology, biomedical research, clinical and pharmaceutical microbiology, pharmaceutical biotechnology, medicinal chemistry, phytochemistry and nutraceuticals.
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