基于共振能量转移和“1,8-萘酰亚胺-苯基吡啶”二元二聚胺受体的水溶液中铜(II)阳离子的选择性和敏感比例荧光探针

IF 4.5 3区 工程技术 Q2 ENGINEERING, CHEMICAL
Pavel A. Panchenko, Marina A. Pavlova, Anastasija V. Efremenko, Uliana A. Kutsevalova, Maria A. Ustimova, Alexey V. Feofanov, Yuri V. Fedorov, Olga A. Fedorova
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引用次数: 0

摘要

考虑到Cu2+通常作为一种有效的荧光猝灭剂,开发用于水溶液和生物系统中Cu2+的比例荧光探针仍然是一项具有挑战性的任务。本文采用叠氮-炔咔嗒反应制备了一种新型的含能给体萘酰亚胺和能受体苯基吡啶发色团的NI-SP二元化合物。利用吸收光谱和荧光光谱研究了NI-SP的光物理性质及其与Cu2+的配位。在NI-SP溶液中加入Cu2+后,苯乙烯吡啶(600 nm)的长波发射峰被淬灭,而萘酰亚胺(450 nm)的荧光由于(NI-SP)·Cu2+配合物中发色团之间的共振能量转移效率降低而增强。观察到的光谱变化可以通过登记荧光强度比I450/I600来实现Cu2+的比例检测。在测试条件下,探针对Cu2+表现出较高的选择性。检测限为120 nmol·L−1,(NI-SP)·Cu2+的稳定常数为3.0 × 106 L·mol−1。生物成像实验表明,NI-SP可以穿透人肺腺癌A549细胞,在线粒体内蓄积,并通过苯乙烯吡啶片段荧光强度的变化响应Cu2+的存在。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Selective and sensitive ratiometric fluorescent probe for copper(II) cations in an aqueous solution based on resonance energy transfer and “1,8-naphthalimide–styrylpyridine” dyad bearing dipicolylamine receptor

Development of ratiometric fluorescent probes for Cu2+ in aqueous solutions and biological systems remains the challenging task, given that Cu2+ commonly acts as an efficient fluorescence quencher. In this work, a novel dyad compound NI-SP bearing energy donor naphthalimide and energy acceptor styrylpyridine chromophore has been prepared using azide-alkyne click reaction. The photophysical properties of NI-SP and its coordination with Cu2+ have been investigated by the absorption and fluorescent spectroscopy. Upon addition of Cu2+ to a solution of NI-SP, the long wavelength emission peak of styrylpyridine (600 nm) was quenched, whereas the fluorescence of naphthalimide (450 nm) was enhanced due to a decrease in resonance energy transfer efficiency between the chromophores in the (NI-SP)·Cu2+ complex. The observed spectral changes enable ratiometric detection of Cu2+ by the registration of the ratio of fluorescence intensities I450/I600. The probe exhibited high selectivity toward Cu2+ in the tested conditions. The detection limit was determined at 120 nmol·L−1, and the stability constant for (NI-SP)·Cu2+ was found to be 3.0 × 106 L·mol−1. Bioimaging experiments showed the NI-SP could penetrate human lung adenocarcinoma A549 cells, accumulate in mitochondria, and respond to the presence of Cu2+ via the changes in the fluorescence intensity of styrylpyridine fragment.

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来源期刊
CiteScore
7.60
自引率
6.70%
发文量
868
审稿时长
1 months
期刊介绍: Frontiers of Chemical Science and Engineering presents the latest developments in chemical science and engineering, emphasizing emerging and multidisciplinary fields and international trends in research and development. The journal promotes communication and exchange between scientists all over the world. The contents include original reviews, research papers and short communications. Coverage includes catalysis and reaction engineering, clean energy, functional material, nanotechnology and nanoscience, biomaterials and biotechnology, particle technology and multiphase processing, separation science and technology, sustainable technologies and green processing.
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