M. G. Ratushnyak, D. A. Shaposhnikova, D. F. Gubani, K. V. Kondratyev
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Characterization of Stem Cell Exosomes Isolated by Ultracentrifugation, Depth Filtration, and Precipitation with Polyethylene Glycol
The biological activity of exosomes secreted by neural (NSC) and mesenchymal stem cells (MSC) isolated from a SC conditioned medium using differential ultracentrifugation, depth filtration, and sedimentation with polyethylene glycol is characterized and compared. The obtained preparations are characterized by size, presence of specific exosomal markers, and protein concentration. The biological activity of exosomes is assessed by their ability to increase the survival and clonogenic activity of irradiated NSCs. The ability of mouse SC exosomes isolated with all these methods to increase the survival and clonogenic activity of irradiated NSCs is shown, but NSC exosomes obtained by ultracentrifugation exhibited the highest efficiency. Any of these methods can be used to obtain biologically active exosomes.
期刊介绍：
Nanobiotechnology Reports publishes interdisciplinary research articles on fundamental aspects of the structure and properties of nanoscale objects and nanomaterials, polymeric and bioorganic molecules, and supramolecular and biohybrid complexes, as well as articles that discuss technologies for their preparation and processing, and practical implementation of products, devices, and nature-like systems based on them. The journal publishes original articles and reviews that meet the highest scientific quality standards in the following areas of science and technology studies: self-organizing structures and nanoassemblies; nanostructures, including nanotubes; functional and structural nanomaterials; polymeric, bioorganic, and hybrid nanomaterials; devices and products based on nanomaterials and nanotechnology; nanobiology and genetics, and omics technologies; nanobiomedicine and nanopharmaceutics; nanoelectronics and neuromorphic computing systems; neurocognitive systems and technologies; nanophotonics; natural science methods in a study of cultural heritage items; metrology, standardization, and monitoring in nanotechnology.
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