Bonghyun Jo, Youngwon Ju, Hee-Won Shin, Joohoon Kim, You Jin Jeong, Tae Kyu Ahn, Hyun Suk Jung
{"title":"枯草芽孢杆菌内生孢子荧光检测的双波长传感器系统","authors":"Bonghyun Jo, Youngwon Ju, Hee-Won Shin, Joohoon Kim, You Jin Jeong, Tae Kyu Ahn, Hyun Suk Jung","doi":"10.1007/s11814-025-00442-z","DOIUrl":null,"url":null,"abstract":"<div><p>Biological threats to military and civilian sectors underscore the need for compact, cost-effective, and durable sensor systems capable of the sensitive and selective detection of bio-threat agents. Fluorescence-based techniques, particularly those employing UV light, have proven effective for detecting biological fluorophores such as proteins and cofactors. However, conventional laser-induced fluorescence (LIF) systems, while highly sensitive, are bulky, expensive, and require significant power and maintenance. To address these limitations, light-emitting diodes (LEDs) have emerged as a promising alternative, offering compact, robust, and low-maintenance solutions. Recent advancements in UV LEDs, spanning 200–400 nm, align with the excitation of natural fluorophores found in bacteria, spores, and viruses, enabling the development of practical fluorescence sensors. In this study, we developed a dual-wavelength fluorescence sensor system employing UV LEDs at 280 nm and 365 nm for the selective detection of Bacillus subtilis endospores, a surrogate for the pathogenic Bacillus anthracis. The system integrates optimized optical lenses and a bio-cell utilizing replaceable quartz sample tubes to minimize contamination and enhance usability. The sensor demonstrated selective detection at concentrations as low as 10<sup>7</sup> spores/mL. This portable, cost-effective system provides a practical solution for rapid and reliable detection of biological threats, meeting critical field requirements for size, weight, and durability. </p></div>","PeriodicalId":684,"journal":{"name":"Korean Journal of Chemical Engineering","volume":"42 8","pages":"1783 - 1792"},"PeriodicalIF":3.2000,"publicationDate":"2025-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s11814-025-00442-z.pdf","citationCount":"0","resultStr":"{\"title\":\"Dual-Wavelength Sensor System for Fluorescence Detection of Bacillus subtilis Endospores\",\"authors\":\"Bonghyun Jo, Youngwon Ju, Hee-Won Shin, Joohoon Kim, You Jin Jeong, Tae Kyu Ahn, Hyun Suk Jung\",\"doi\":\"10.1007/s11814-025-00442-z\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Biological threats to military and civilian sectors underscore the need for compact, cost-effective, and durable sensor systems capable of the sensitive and selective detection of bio-threat agents. Fluorescence-based techniques, particularly those employing UV light, have proven effective for detecting biological fluorophores such as proteins and cofactors. However, conventional laser-induced fluorescence (LIF) systems, while highly sensitive, are bulky, expensive, and require significant power and maintenance. To address these limitations, light-emitting diodes (LEDs) have emerged as a promising alternative, offering compact, robust, and low-maintenance solutions. Recent advancements in UV LEDs, spanning 200–400 nm, align with the excitation of natural fluorophores found in bacteria, spores, and viruses, enabling the development of practical fluorescence sensors. In this study, we developed a dual-wavelength fluorescence sensor system employing UV LEDs at 280 nm and 365 nm for the selective detection of Bacillus subtilis endospores, a surrogate for the pathogenic Bacillus anthracis. The system integrates optimized optical lenses and a bio-cell utilizing replaceable quartz sample tubes to minimize contamination and enhance usability. The sensor demonstrated selective detection at concentrations as low as 10<sup>7</sup> spores/mL. This portable, cost-effective system provides a practical solution for rapid and reliable detection of biological threats, meeting critical field requirements for size, weight, and durability. </p></div>\",\"PeriodicalId\":684,\"journal\":{\"name\":\"Korean Journal of Chemical Engineering\",\"volume\":\"42 8\",\"pages\":\"1783 - 1792\"},\"PeriodicalIF\":3.2000,\"publicationDate\":\"2025-04-25\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://link.springer.com/content/pdf/10.1007/s11814-025-00442-z.pdf\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Korean Journal of Chemical Engineering\",\"FirstCategoryId\":\"5\",\"ListUrlMain\":\"https://link.springer.com/article/10.1007/s11814-025-00442-z\",\"RegionNum\":4,\"RegionCategory\":\"工程技术\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"CHEMISTRY, MULTIDISCIPLINARY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Korean Journal of Chemical Engineering","FirstCategoryId":"5","ListUrlMain":"https://link.springer.com/article/10.1007/s11814-025-00442-z","RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"CHEMISTRY, MULTIDISCIPLINARY","Score":null,"Total":0}
Dual-Wavelength Sensor System for Fluorescence Detection of Bacillus subtilis Endospores
Biological threats to military and civilian sectors underscore the need for compact, cost-effective, and durable sensor systems capable of the sensitive and selective detection of bio-threat agents. Fluorescence-based techniques, particularly those employing UV light, have proven effective for detecting biological fluorophores such as proteins and cofactors. However, conventional laser-induced fluorescence (LIF) systems, while highly sensitive, are bulky, expensive, and require significant power and maintenance. To address these limitations, light-emitting diodes (LEDs) have emerged as a promising alternative, offering compact, robust, and low-maintenance solutions. Recent advancements in UV LEDs, spanning 200–400 nm, align with the excitation of natural fluorophores found in bacteria, spores, and viruses, enabling the development of practical fluorescence sensors. In this study, we developed a dual-wavelength fluorescence sensor system employing UV LEDs at 280 nm and 365 nm for the selective detection of Bacillus subtilis endospores, a surrogate for the pathogenic Bacillus anthracis. The system integrates optimized optical lenses and a bio-cell utilizing replaceable quartz sample tubes to minimize contamination and enhance usability. The sensor demonstrated selective detection at concentrations as low as 107 spores/mL. This portable, cost-effective system provides a practical solution for rapid and reliable detection of biological threats, meeting critical field requirements for size, weight, and durability.
期刊介绍:
The Korean Journal of Chemical Engineering provides a global forum for the dissemination of research in chemical engineering. The Journal publishes significant research results obtained in the Asia-Pacific region, and simultaneously introduces recent technical progress made in other areas of the world to this region. Submitted research papers must be of potential industrial significance and specifically concerned with chemical engineering. The editors will give preference to papers having a clearly stated practical scope and applicability in the areas of chemical engineering, and to those where new theoretical concepts are supported by new experimental details. The Journal also regularly publishes featured reviews on emerging and industrially important subjects of chemical engineering as well as selected papers presented at international conferences on the subjects.