基于免洗磁性纳米颗粒高效合成诊断核酸提取用磁性二氧化硅颗粒

IF 3.2 4区 工程技术 Q2 CHEMISTRY, MULTIDISCIPLINARY
Ahadi Damar Prasetya, Grace Tj Sulungbudi,  Muflikhah,  Mujamilah, Andon Insani, Wildan Zakiah Lubis, Uus Saepulloh,  Yuliana, Ellis Dwi Ayuningsih
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引用次数: 0

摘要

磁性二氧化硅颗粒(MAGSi)已广泛用于快速、大规模的核酸提取。然而,合成过程通常涉及洗涤步骤,这将产生化学废物,耗时。在本研究中,我们通过消除磁性纳米颗粒生产中的洗涤步骤来合成磁性二氧化硅细颗粒,从而提高了环境可持续性并缩短了合成时间。首先,在不洗涤Fe3O4纳米颗粒的情况下,通过Stöber法在二氧化硅涂层上继续共沉淀法合成了平均尺寸约为100 nm的磁性纳米颗粒(MNP)(基于动态光散射(DLS)数据)。与水洗MNP相比,未水洗MNP具有相似的磁化强度,未水洗MNP磁化强度为47.85 emu/g,水洗MNP磁化强度为47.10 emu/g。结果表明,在Fe3O4:SiO2质量比为1:8的情况下,MNP的磁化强度降低了5.00 emu/g。与水洗MNP合成的MAGSi相比,未水洗MNP制备的MAGSi尺寸更大(约1700 nm,而DLS数据为320 nm), SiO2 FTIR峰更明显,并且由于SiO2层更厚,磁化强度更低。经PCR和qRT-PCR验证,使用未洗涤的MNP合成的MAGSi仍然能够有效地从病毒(SARS-CoV-2和犬瘟热病毒)和细菌(钩端螺旋体)样品中提取RNA和DNA。这项工作介绍了合成MAGSi的实用和可持续的方法,展示了它们在核酸提取中的实用性而不影响性能。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Efficient Synthesis of Magnetic-Silica Particles for Nucleic Acid Extraction in Diagnostics Based on Washing-Free Magnetic Fine Nanoparticles

Efficient Synthesis of Magnetic-Silica Particles for Nucleic Acid Extraction in Diagnostics Based on Washing-Free Magnetic Fine Nanoparticles

Magnetic-silica particles (MAGSi) have been widely used for rapid and large-scale nucleic acid extraction. However, the synthesis procedure normally involves washing steps, which will produce chemical waste and are time-consuming. In this study, we synthesized magnetic-silica fine particles by eliminating the washing step in magnetic nanoparticle production, thereby improving environmental sustainability and reducing synthesis time. First, magnetic nanoparticles (MNP) with sizes averaging around 100 nm (based on dynamic light scattering (DLS) data) were synthesized via co-precipitation continued with silica coating by the Stöber method, without washing the Fe3O4 nanoparticles. Compared to the washed MNP, the unwashed MNP exhibited similar magnetization, with 47.85 emu/g for the unwashed and 47.10 emu/g for the washed MNP. The unwashed MNP was also successfully coated with silica, which resulted in a decrease in magnetization (5.00 emu/g) with a Fe3O4:SiO2 mass ratio of 1:8. Compared to MAGSi synthesized by washed MNP, the unwashed MNP produced MAGSi with a larger size (~ 1700 nm compared to 320 nm based on the DLS data), more pronounced SiO2 FTIR peaks, and lower magnetization due to a thicker SiO2 layer. The MAGSi synthesized using the unwashed MNP is still effectively able to extract RNA and DNA from viral (SARS-CoV-2 and Canine Distemper Virus) and bacterial (Leptospira spp.) samples, as validated through PCR and qRT-PCR. This work introduces a practical and sustainable approach for synthesizing MAGSi, demonstrating their utility in nucleic acid extraction without compromising performance.

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来源期刊
Korean Journal of Chemical Engineering
Korean Journal of Chemical Engineering 工程技术-工程:化工
CiteScore
4.60
自引率
11.10%
发文量
310
审稿时长
4.7 months
期刊介绍: The Korean Journal of Chemical Engineering provides a global forum for the dissemination of research in chemical engineering. The Journal publishes significant research results obtained in the Asia-Pacific region, and simultaneously introduces recent technical progress made in other areas of the world to this region. Submitted research papers must be of potential industrial significance and specifically concerned with chemical engineering. The editors will give preference to papers having a clearly stated practical scope and applicability in the areas of chemical engineering, and to those where new theoretical concepts are supported by new experimental details. The Journal also regularly publishes featured reviews on emerging and industrially important subjects of chemical engineering as well as selected papers presented at international conferences on the subjects.
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