Yuri Pereira de Souza, Angélica Castanheira de Oliveira, Lucia Helena Pinto Bastos, André Victor Sartori, Bernardete Ferraz Spisso
{"title":"UHPLC-MS/MS同时测定鸡蛋中农药、兽药和真菌毒素的微型两步中性酸化乙腈萃取方法的建立与验证","authors":"Yuri Pereira de Souza, Angélica Castanheira de Oliveira, Lucia Helena Pinto Bastos, André Victor Sartori, Bernardete Ferraz Spisso","doi":"10.1007/s12161-025-02800-3","DOIUrl":null,"url":null,"abstract":"<div><p>This study aimed to develop and validate an attractive two-step neutral and acidified acetonitrile extraction method for the simultaneous determination of pesticides, veterinary drugs, and mycotoxins in eggs by UHPLC-MS/MS. A simple miniaturized solvent extraction method followed by extract concentration was proposed for sample treatment without the need for subsequent extract cleanup steps. Satisfactory recoveries (70 to 120%), with relative standard deviations below lower than 22%, were achieved for 285 compounds. The limits of quantification ranged from 0.1 to 50 µg kg<sup>−1</sup>. Calibration plots with <i>R</i><sup>2</sup> ≥ 0.99 were obtained for all analytes. The validated method was successfully employed for the analysis of egg samples collected from local supermarkets in Rio de Janeiro, Brazil. The results obtained demonstrate its high-throughput and suitability for routine analysis.</p></div>","PeriodicalId":561,"journal":{"name":"Food Analytical Methods","volume":"18 7","pages":"1339 - 1354"},"PeriodicalIF":3.0000,"publicationDate":"2025-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Development and Validation of a Miniaturized Two-Step Neutral and Acidified Acetonitrile Extraction Method for Simultaneous Determination of Pesticides, Veterinary Drugs, and Mycotoxins in Eggs by UHPLC-MS/MS\",\"authors\":\"Yuri Pereira de Souza, Angélica Castanheira de Oliveira, Lucia Helena Pinto Bastos, André Victor Sartori, Bernardete Ferraz Spisso\",\"doi\":\"10.1007/s12161-025-02800-3\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>This study aimed to develop and validate an attractive two-step neutral and acidified acetonitrile extraction method for the simultaneous determination of pesticides, veterinary drugs, and mycotoxins in eggs by UHPLC-MS/MS. A simple miniaturized solvent extraction method followed by extract concentration was proposed for sample treatment without the need for subsequent extract cleanup steps. Satisfactory recoveries (70 to 120%), with relative standard deviations below lower than 22%, were achieved for 285 compounds. The limits of quantification ranged from 0.1 to 50 µg kg<sup>−1</sup>. Calibration plots with <i>R</i><sup>2</sup> ≥ 0.99 were obtained for all analytes. The validated method was successfully employed for the analysis of egg samples collected from local supermarkets in Rio de Janeiro, Brazil. The results obtained demonstrate its high-throughput and suitability for routine analysis.</p></div>\",\"PeriodicalId\":561,\"journal\":{\"name\":\"Food Analytical Methods\",\"volume\":\"18 7\",\"pages\":\"1339 - 1354\"},\"PeriodicalIF\":3.0000,\"publicationDate\":\"2025-04-16\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Food Analytical Methods\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://link.springer.com/article/10.1007/s12161-025-02800-3\",\"RegionNum\":3,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"FOOD SCIENCE & TECHNOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Food Analytical Methods","FirstCategoryId":"97","ListUrlMain":"https://link.springer.com/article/10.1007/s12161-025-02800-3","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"FOOD SCIENCE & TECHNOLOGY","Score":null,"Total":0}
Development and Validation of a Miniaturized Two-Step Neutral and Acidified Acetonitrile Extraction Method for Simultaneous Determination of Pesticides, Veterinary Drugs, and Mycotoxins in Eggs by UHPLC-MS/MS
This study aimed to develop and validate an attractive two-step neutral and acidified acetonitrile extraction method for the simultaneous determination of pesticides, veterinary drugs, and mycotoxins in eggs by UHPLC-MS/MS. A simple miniaturized solvent extraction method followed by extract concentration was proposed for sample treatment without the need for subsequent extract cleanup steps. Satisfactory recoveries (70 to 120%), with relative standard deviations below lower than 22%, were achieved for 285 compounds. The limits of quantification ranged from 0.1 to 50 µg kg−1. Calibration plots with R2 ≥ 0.99 were obtained for all analytes. The validated method was successfully employed for the analysis of egg samples collected from local supermarkets in Rio de Janeiro, Brazil. The results obtained demonstrate its high-throughput and suitability for routine analysis.
期刊介绍：
Food Analytical Methods publishes original articles, review articles, and notes on novel and/or state-of-the-art analytical methods or issues to be solved, as well as significant improvements or interesting applications to existing methods. These include analytical technology and methodology for food microbial contaminants, food chemistry and toxicology, food quality, food authenticity and food traceability. The journal covers fundamental and specific aspects of the development, optimization, and practical implementation in routine laboratories, and validation of food analytical methods for the monitoring of food safety and quality.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
