Fabrication and evaluation of AMPS-based enteric-coated hydrogel beads: a promising tool for colon targeting

Targeted drug delivery to the colon offers a promising approach to enhance therapeutic efficacy while minimizing systemic side effects. Our goal in this study was to create a pH-responsive, crosslinked hydrogel matrix for colonic drug release under control. Hydrogels based on poly (2-acrylamido-2-methylpropanesulfonic acid) (poly (AMPS)) were created by adjusting the amounts of the crosslinker (N, N’-methylene bisacrylamide, MBA) and monomer (AMPS). The resultant solidified hydrogel was converted into hydrogel beads. Several analytical approaches were used to thoroughly analyse the hydrogel beads’ structural, physicochemical, and pharmacokinetic characteristics. The existence of the intended chemical connections was confirmed by Fourier Transform Infrared Spectroscopy, and swelling investigations showed that swelling behavior was pH-dependent, with higher pH values causing more swelling. The investigation of drug entrapment efficiency revealed that nimesulide, a model drug, was effectively loaded into the hydrogel matrix. Studies on in vitro drug release showed pH-responsive and prolonged release characteristics, suggesting the possibility of specific delivery of drugs to the colon. Furthermore, the hydrogel beads’ capacity to expand and their biocompatibility were confirmed by in vivo testing in rabbit models, where no acute toxicity was noted. The results of our study highlight the potential therapeutic benefits of using pH-responsive poly (AMPS) based hydrogels for targeted medication delivery to the colon, particularly in the treatment of colorectal cancer and associated gastrointestinal problems.

Graphical Abstract