山核桃内生真菌西多曲霉EF 375的多功能果胶酶:工艺优化、表征及应用前景

IF 2.3 4区 化学 Q3 CHEMISTRY, PHYSICAL
M R Revathy, Anjali S. Mohan, Dhanya Kesavan, Hafsa Kattatheyil, S Neelima, M V Anju, Rosamma Philip
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引用次数: 0

摘要

微生物果胶酶属于水解酶家族,在各种工业应用中发挥着至关重要的作用,包括食品和饮料加工、纺织制造和废水处理。本研究研究了从红树林植物Ceriops tagal中分离的内生真菌Aspergillus sydowii EF 375中提取的果胶酶的生产、纯化、生化特性和潜在应用前景。利用响应面法(RSM)优化果胶酶产量,使酶产量提高2.2倍。对该酶进行纯化,回收率为3.2%,比活性为73.05 U/mg。酶谱分析证实纯化的果胶酶分子量约为43 kDa。动力学研究表明,该化合物的最大反应速度(Vmax)为161.29µmol/min, Michaelis常数(Km)为0.177 mg/ml,具有较高的底物亲和性。该酶在50℃、pH为5时表现出最佳的催化效率。K +、Cu2 +、Fe2 +、Mg2 +、Mn2 +、Pb2 +和Zn2 +离子(浓度高达10 mM)的存在显著增强了酶的活性,而Li +、Hg2 +和Ni2 +起到了抑制剂的作用。功能应用表明,纯化的果胶酶显著提高了果汁的透明度,包括苹果、葡萄、菠萝和橙汁。此外,该酶显示出抑制细菌生物膜形成的潜力,突出了其在抗菌和生物技术干预方面的适用性。图形抽象
本文章由计算机程序翻译,如有差异,请以英文原文为准。
A Multifunctional Pectinase from the Manglicolous Endophytic Fungus, Aspergillus sydowii EF 375: Process Optimization, Characterization and Application Potentials

Microbial pectinases, classified within the hydrolase family, play a crucial role in various industrial applications, including food and beverage processing, textile manufacturing, and wastewater treatment. This study investigates the production, purification, biochemical characterization, and potential applications of pectinase derived from the endophytic fungal strain Aspergillus sydowii EF 375, isolated from the mangrove plant Ceriops tagal. Pectinase production was optimized using Response Surface Methodology (RSM), resulting in a 2.2-fold enhancement in enzyme production. The enzyme was subsequently purified, achieving a recovery yield of 3.2% and a specific activity of 73.05 U/mg. Zymogram analysis confirmed the purified pectinase to have a molecular weight of approximately 43 kDa. Kinetic studies revealed a maximum reaction velocity (Vmax) of 161.29 µmol/min and a Michaelis constant (Km) of 0.177 mg/ml, indicating high substrate affinity. The enzyme exhibited optimal catalytic efficiency at 50 °C and pH 5. The activity of the enzyme was notably enhanced by the presence of K⁺, Cu2⁺, Fe2⁺, Mg2⁺, Mn2⁺, Pb2⁺, and Zn2⁺ ions (at concentrations up to 10 mM), while Li⁺, Hg2⁺, and Ni2⁺ acted as inhibitors. Functional applications demonstrated that the purified pectinase significantly improved the clarity of fruit juices, including apple, grape, pineapple, and orange. Furthermore, the enzyme exhibited potential in inhibiting bacterial biofilm formation, highlighting its applicability in antimicrobial and biotechnological interventions.

Graphical Abstract

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来源期刊
Catalysis Letters
Catalysis Letters 化学-物理化学
CiteScore
5.70
自引率
3.60%
发文量
327
审稿时长
1 months
期刊介绍: Catalysis Letters aim is the rapid publication of outstanding and high-impact original research articles in catalysis. The scope of the journal covers a broad range of topics in all fields of both applied and theoretical catalysis, including heterogeneous, homogeneous and biocatalysis. The high-quality original research articles published in Catalysis Letters are subject to rigorous peer review. Accepted papers are published online first and subsequently in print issues. All contributions must include a graphical abstract. Manuscripts should be written in English and the responsibility lies with the authors to ensure that they are grammatically and linguistically correct. Authors for whom English is not the working language are encouraged to consider using a professional language-editing service before submitting their manuscripts.
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